Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Effects of ethanol extract of Nelumbo nucifera leaves on Anti-oxidation and Type I Procollagen Expression in CCD-986sk Cells

연잎 에탄올 추출물이 피부 주름에 미치는 영향

  • Yang, Woong-Mo (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Hyang-Mi (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Chang, Mun-Seog (Department of Medicine, Division of Newborn Medicine, Children's Hospital and Harvard Medical School) ;
  • Park, Wan-Su (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Won-Nam (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Kim, San-Woong (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Choi, Dong-Gi (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Hak-Chul (Department of Oriental Preventive Medicine, College of Oriental Medicine, Dong-Eui University) ;
  • Kim, Yun-Kyung (College of Pharmacy, Wonkwang University) ;
  • Park, Seong-Kyu (Department of Prescriptionology, College of Oriental Medicine, Kyung Hee University)
  • 양웅모 (경희대학교 한의과대학 처방제형학교실) ;
  • 김향미 (경희대학교 한의과대학 처방제형학교실) ;
  • 장문석 (하버드대학교 의과대학 소아병원) ;
  • 박완수 (경희대학교 한의과대학 처방제형학교실) ;
  • 김원남 (경희대학교 한의과대학 처방제형학교실) ;
  • 김산웅 (경희대학교 한의과대학 처방제형학교실) ;
  • 최동기 (경희대학교 한의과대학 처방제형학교실) ;
  • 이학철 (동의대학교 한의과대학 예방의학교실) ;
  • 김윤경 (원광대학교 약학대학 한약학과) ;
  • 박성규 (경희대학교 한의과대학 처방제형학교실)
  • Published : 2006.12.30
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Abstract

The natural plants were screened for new cosmetic materials. Many parts of Nelumbo nucifera has been investigated for anti-wrinkling and anti-furrowing effects. The leaves. flowers and stamens of Nelumbo nucifera were extracted with ethanol or distilled water. The effects of cell viability and $H_2O_2-induced$ cytotoxicity were studied on CCD-986sk human fibroblast cell line by MTT assay. We measured various effects related to skin such as the synthesis of MMP-1 and type I procollagen. The ethanol extract of Nelumbo nucifera leaves (ENL) at concentrations of 30 ${\mu}g/ml$ showed maximum cell viability on CCD-986sk cells by 129.70%. and $H_2O_2-induced$ cytotoxicity was blocked by ENL (5. 10. 20. 30. and 40 ${\mu}g/ml$) concentration-dependently. The synthesis of MMP-1 and type I procollagen were positive in ENL. These results suggest that ENL has anti-oxidant and anti-furrowing effects.

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