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Improvement of Physiological Activity of the Ethanol Extract from Boiled-water of Enteroctopus dofleini by Gamma Irradiation

감마선 조사에 의한 문어 자숙액 에탄올 추출물의 생리활성 개선

  • Kim, Hyun-Joo (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Choi, Jong-Il (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Lee, Hee-Sub (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Hun (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Byun, Myung-Woo (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute) ;
  • Chun, Byung-Soo (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Ahn, Dong-Hyun (Faculty of Food Science and Biotechnology, Pukyong National University) ;
  • Yook, Hong-Sun (Dept. of Food and Nutrition, Chungnam National University) ;
  • Lee, Ju-Woon (Team for Radiation Food Science and Biotechnology, Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute)
  • 김현주 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 최종일 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 이희섭 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 김재훈 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 변명우 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실) ;
  • 전병수 (부경대학교 식품생명공학부) ;
  • 안동현 (부경대학교 식품생명공학부) ;
  • 육홍선 (충남대학교 식품영양학과) ;
  • 이주운 (한국원자력연구원 정읍방사선과학연구소 방사선식품생명공학연구실)
  • Published : 2007.12.31

Abstract

Physiological activities of the 70% ethanol extract from boiled-water of Enteroctopus dofleini (BE) upon a gamma irradiation were determined to examine the possible use of the food processing by-product as a functional material for food or cosmetic composition. Electron donating ability and the content of total phenolic compounds increased from 65% to 72% and from 406 ppm to 476 ppm, respectively, by 10 kGy gamma irradiation. A Salmonella mutagenicity assay indicated that the gamma-irradiated BE did not show any mutagenicity. Tyrosinase inhibition activity of the irradiated BE also increased from 57% to 86% with irradiation doses. These results show that irradiation can be used to increase the physiological activities of BE for its potential use as food or cosmetic materials.

수산가공 폐기물 중 하나인 문어 자숙액을 이용하여 천연 기능성 소재로 개발하기 위한 기초자료를 마련하고자 감마선 조사(0, 1, 3, 5, 7, 10 kGy)에 의한 문어 자숙액 에탄올 추출물의 생리활성 변화를 알아보았다. 감마선 조사한 문어 자숙액의 전자공여능 및 총 폴리페놀 함량을 측정한 결과 전자공여능의 경우 조사선량이 증가함에 따라 65.87%에서 72.88%로 증가하였으며, 총 폴리페놀 함량의 경우는 406.32 ppm에서 476.25 ppm으로 증가하는 경향을 보였다. 그리고 tyrosinase 저해 효과를 측정한 결과 조사선량이 증가함에 따라 57.49%에서 86.27%로 활성이 증가한 것으로 확인하였다. 따라서 감마선 조사기술은 수산가공 폐자원인 문어 자숙액의 생리활성을 개선하여 식품 및 공중보건산물의 첨가제로 활용할 수 있다고 사료된다.

Keywords

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