Effect of ${\alpha}-Amylase$ Treatment of Brown Rice(Goami) Alcohol Fermentation By-Product

현미(고아미) 알코올발효 부산물의 ${\alpha}-amylase$처리 효과

  • Woo, Seung-Mi (Department of Food Science and Technology, Keimyung University and Keimyung Foodex Co., Ltd) ;
  • Kim, Tae-Young (Rural Resources Development Institute, NIAST, RDA) ;
  • Yeo, Soo-Hwan (Rural Resources Development Institute, NIAST, RDA) ;
  • Kim, Sang-Burm (Rural Resources Development Institute, NIAST, RDA) ;
  • Kim, Mi-Hyun (Division of Food Nutrition and Cooking, Taegu Science College) ;
  • Woo, Sang-Chel (Department of Fire Safety Management, Daegu Health College) ;
  • Jeong, Yong-Jin (Department of Food Science and Technology, Keimyung University and Keimyung Foodex Co., Ltd)
  • 우승미 (계명대학교 식품가공학과 및 (주)계명푸덱스) ;
  • 김태영 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 여수환 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 김상범 (농촌진흥청 농업과학기술원 농촌자원개발연구소) ;
  • 김미현 (대구과학대학 식품영양조리계열) ;
  • 우상철 (대구보건대학 소방안전과) ;
  • 정용진 (계명대학교 식품가공학과 및 (주)계명푸덱스)
  • Published : 2007.12.31

Abstract

To utilize the non-heat treated alcoholic by-products of brown rice(Goami) as food sources, the quality characteristics change according to the treatment conditions of ${\alpha}-amylase$ were evaluated. It resulted that the increase of hydrolysis temperature correspondingly increased the soluble solids, total dietary fiber and total sugar in the by-products of Goami, and the highest reducing sugar content was observed at $80^{\circ}C$. The free amino acids contents were tended to slowly decrease by the hydrolysis temperature more than $70^{\circ}C$, and the highest content of oligosaccharides were detected at the hydrolysis temperature of $80^{\circ}C$. The soluble solid according to the ${\alpha}-amylase$ concentration resulted to increase with the increase of the enzyme concentration and the total dietary fiber revealed similarly showing approximately 0.65%. The high content of reducing sugars was observed at the enzyme concentration around 0.08%(v/w). Total sugars and oligosaccharides contents tend to increase as the concentration of enzyme increased, and the content of oligosaccharides acquired at the enzyme concentration more than 0.10%(v/w) maintained to show rather similar contents. The soluble solids and total dietary fiber by hydrolysis time were found to show 6.66% and 0.65%, respectively at more than 60 min of hydrolysis, and the reducing sugars and total sugars were found to be 3,600 and 4,800 mg% in all treatment groups showing no significant difference. The content of oligosaccharides was increased with the increase of hydrolysis time, and the content was similar at more than 90 min of hydrolysis by ranging around 2,100 mg%. Based upon these results, the by-products of Goami are expected to be used as various food sources showing the highest dietary fiber and oligosaccharides contents by the hydrolysis at $80^{\circ}C$ for 90 min with the addition of 0.10%(v/w) of ${\alpha}-amylase$.

현미(고아미) 비열처리 알코올발효 부산물을 식품소재로 활용하고자 ${\alpha}-amylase$처리조건에 따른 품질특성 변화를 조사하였다. 그 결과, 가수분해 온도에 따른 고아미 부산물의 가용성 고형분, 총식이섬유소 및 총당은 온도가 높을수록 증가하였으며, 환원당은 $80^{\circ}C$에서 가장 높게 나타났다. 가수분해 온도 $70^{\circ}C$이상에서는 유리아미노산이 점차 감소하는 경향이었으며, 올리고당은 $80^{\circ}C$에서 가수분해 하였을 때 가장 많이 검출되었다. ${\alpha}-amylase$ 농도에 따른 가용성 고형분은 효소제 농도가 높을수록 함량이 증가하였고 총식이섬유소는 약 0.65%로 비슷하게 나타났다. 환원당은 효소제 농도 0.08%(v/w)전후에서 높은 함량을 나타내었다. 총당 및 올리고당은 효소제 농도가 높을수록 증가하는 경향이었으며, 올리고당은 효소제 농도 0.10%(v/w)이상에서는 비슷한 함량을 나타내었다. 가수분해 시간에 따른 가용성 고형분 및 총식이섬유소는 60분 이상에서 각각 약 6.65 및 0.65%로 나타났으며, 환원당 및 총당은 모든 처리구에서 각각 약 3,600 및 4,800 mg%로 나타나 가수분해 시간에 따른 큰 차이는 없었다. 올리고당은 가수분해 시간이 경과할수록 증가하였으며 90분 이상에서는 약 2,100 mg%로 비슷한 함량을 나타내었다. 이상의 결과, 고아미 부산물은 ${\alpha}-amylase$ 0.10%(v/w)를 첨가하여 $80^{\circ}C$에서 90분간 가수분해하였을 때 식이섬유소 및 올리고당 함량이 가장 높은 것으로 나타나 식품소재로의 다양한 활용이 기대되었다.

Keywords

References

  1. Na, G.S., Lee, S.K. and Kim, S.Y. (2007) Antioxidative effects and quality characteristics of the rice cultivated by organic farming and ordinary farming. J. Korean Soc. Appl. Biol. Chem., 50, 36-41
  2. Jeong, H.U. (2003) Science and health of rice; Current status of processed foods in rice. Korean J. Food Preserv., 10, 71-77
  3. Lee, E.J., Seo, H.S., Lee, S.Y., Kim, S.H. and Hwang, I.K. (2006) Quality characteristics of black sesame gruel with high dietary fiber rice 'Goami 2'. Korean J. Food Cookery Sci., 22, 940-948
  4. Kim, C., Lee, E.S., Hong, S.T. and Ryu, G.H. (2007) Manufacturing of Goami flakes by using extrusion process. Korean J. Food Sci. Technol., 39, 146-151
  5. Kang, H.J., Hwang, I.K., Kim, K.S. and Choi, H.C. (2003) Comparative structure and physicochemical properties of Ilpumbyeo, a high-quality japonica, and its mutant, Suweon 464. J. Agric. Food Chem., 51, 6598-6603 https://doi.org/10.1021/jf0344946
  6. 아주대학교 의과대학 (2003) 고섬유소쌀(고아미 2호)의 기능성 및 인체 생리활성 효과 규명. 농촌진흥청
  7. Lee, C. and Shin, J.S. (2002) Effects of different fiber content of rice on blood glucose and triglyceride levels in normal subject. J. Korean Soc. Food Sci. Nutr., 31, 1048-1051 https://doi.org/10.3746/jkfn.2002.31.6.1048
  8. Kim, K.S., Kang, H.J., Hwang, I.K. Hwang, H.G. Kim, T.Y. and Choi, H.C. (2004) Comparative ultrastructure of Ilpumbyeo, a high-quality of japonica rice, and its mutant, Suweon 464:Scanning and transmission electron microscopy studies. J. Agric. Food Chem. 52, 3876-3883 https://doi.org/10.1021/jf049767r
  9. Lee, J.H. (2006) The physicochemical and sensory characteristics of rice 'Goami 2' and its products. master's thesis. The Seoul National University. p.85-88
  10. Bae, H.K, Lee, S.Y. and Hwang, S.H. (2005) Response surface analysis and optimization of producing highly valuable mushroom mycelia on the solid-state midium of starch processing waste. Paper presented at Spring Meeting of Korean Society of Environmental Engineers, April 28, Suweon, Korea
  11. Park, C.H., Kim, H.J. and Moon, T.W. (1997) Preparation and physicochemical properties of soluble dietary fiber extracts from soymilk residue at high temperature. Korean J. Food Sci. Technol., 29, 648-656
  12. Vihinen, M. and Mantsala, P. (1989) Microbial maylolytic enzymes. Crit. Rev. Biochem. Mol. Biol., 24, 329-418 https://doi.org/10.3109/10409238909082556
  13. Lim, J.K., Lee, H.S., Kim, Y.J., Bae, S.S., Jeon, J.H., Kang, S.G. and Lee, J.H. (2007) Critical factors to high thermostability of an ${\alpha}$-amylase from hyperthermophilic archaeon Thermococcus onnurineus NA1. J. Microbiol. Biotechnol., 17, 1242-1248
  14. Dziezak, J.D. (1991) Enzymes: catalyst for food process. Food Technol., 45, 78-85
  15. Yoon, S.H., Kim, M.J., Kim, J.W., Kwon, K.S., Lee, Y.W. and Park, K.H. (1995) Purification and characterization of a novel malto-oligosaccharides forming ${\alpha}$-amylase from Bacillus sp. SUH4-2. Korean Soc. Microbiol. Biotechnol., 23, 573-579
  16. Woo, S.M., Kim, T.Y., Yeo, S.H., Kim, S.B., Kim, J.S., Kim, M.H. and Jeong, T.J. (2007) Quality characteristics of alcohol fermentation broth and by-product of brown rice varieties. Korean J. Food Preserv., 14, 557-563
  17. Gu, Y.A., Jang, S.Y., Park, N.Y., Mun, C.R., Kim, O.M. and Jeong, Y.J. (2006) Property changes of mung bean depending on hydrolysis conditions. Korean J. Food Preserv., 13, 563-568
  18. A.O.A.C. (1990) Official Methods of Analysis. 15th ed., Association of Official Analytical Chemists, Washington, D.C., p.210-219
  19. Miller, G.L. (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal. Chem., 31, 426-428 https://doi.org/10.1021/ac60147a030
  20. Yoon, S.R., Jeong, Y.J., Lee, G.D. and Kwon, J.H. (2003) Changes in phenolic compounds properties of rubi fructus extract depending on extraction conditions. J. Korean Soc. Food Sci. Nutr., 32, 338-345 https://doi.org/10.3746/jkfn.2003.32.3.338
  21. A.O.A.C. (1996) Official Methods of Analysis. 15th ed., Association of Official Analytical Chemists, Washington, D.C., p.115
  22. Shin, J.S. and Jeong, Y.J. (2003) Changes in the components of acetic acid fermentation of brown rice using raw starch digesting enzyme. J. Korean Soc. Food Sci. Nutr., 32, 381-387 https://doi.org/10.3746/jkfn.2003.32.3.381
  23. Kim, K.A. (1996) Physicochemical properties of nonwaxy and waxy brown rice flour. Korean J. Soc. Food Sci., 12, 557-561
  24. Youn, K.S. and Choi, Y.H. (1998) The quality characteristics of dried kiwifruit using different drying methods. Korean Soc. Food Engin., 2, 49-54
  25. Kang, H.J., Seo, H.S. and Hwang, I.K. (2004) Comparison of gelatinization and retrogradation characteristics among endosperm mutant rices derived from Ilpumbyeo. Korean J. Food Sci. Technol., 36, 879-884
  26. Ko, Y.R., Kwon, S.H., Choi, J.H., Shon, M.Y. and Park, S.K. (2003) Nitrogen compounds and free amino acids of black bean Kanjang prepared with different cooking conditions of whole black bean. Korean J. Food Preserv., 10, 75-79
  27. Sung, H. S., Kim, W. J. and Yang, C. B. (1985) Effect of extraction temperature and ethanol concentration of nitrogeneous constituents of red ginseng extract. Korean J. Ginseng Sci., 9, 95-103
  28. Akers, A.A. and Hoseney, R.C. (1994) Water-soluble dextrins from $\alpha$-amylase treated bread and their relationship to bread firming. Cereal Chem., 71, 223-226
  29. Ivanova, V.N., Dobreva, E.P. and Emanuilova, E.I. (1993) Purification and characterization of a thermostable alpha-amylase from Bacillus licheniformis. J. Biotechnol. 28, 277-289 https://doi.org/10.1016/0168-1656(93)90176-N
  30. Shin, H.H, Lee, S.H., Park, B.S., Rhim, T.S. and Hwang, J.K. (2003) Solubilization of whole grains by extrusion and enzyme treatment. Korean J. Food Sci. Technol., 35, 849-855
  31. Cha, B.S. (1999) Studies on processing conditions for Nooroong-gi powder by liquefaction and gelatinization of rice powder. Korean J. Soc. Food Sci., 15, 469-474