Food Science and Preservation (한국식품저장유통학회지)

The Korean Society of Food Preservation (한국식품저장유통학회)
권호 표지

Comparison of Antioxidant, Anticancer and Immunomodulating Activities of Extracts from DongChongXiaCao

동충하초의 균사 및 배양액의 항산화, 항암, 면역활성의 비교

  • Heo, Jin-Chul (Department of Food Science & Technology, Kyungpook National University) ;
  • Nam, Sung-Hee (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA) ;
  • Kang, Seok-Woo (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA) ;
  • Hong, In-Pyo (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA) ;
  • Lee, Kwang-Kil (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA) ;
  • Park, Ja-Young (Daegu Research Institute of Public Health & Environment) ;
  • Kim, Kyung-Hae (Department of Food Science & Technology, Kyungpook National University) ;
  • Han, Song-Yi (Department of Food Science & Technology, Kyungpook National University) ;
  • Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
  • 허진철 (경북대학교 식품공학과) ;
  • 남성희 (농업과학기술원 농업생물부) ;
  • 강석우 (농업과학기술원 농업생물부) ;
  • 홍인표 (농업과학기술원 농업생물부) ;
  • 이광길 (농업과학기술원 농업생물부) ;
  • 박자영 (대구보건환경연구원) ;
  • 김경해 (경북대학교 식품공학과) ;
  • 한송이 (경북대학교 식품공학과) ;
  • 이상한 (경북대학교 식품공학과)
  • Published : 2007.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

Biological properties of DongChongXiaCao extracts and culture supernatants were evaluated using DPPH and FRAP (antioxidants), Raw 264.7 (NO production), B16-F1 cells (cell migration activity) and HUVECs (angiogenesis activity). We found that antioxidant activity was higher in mycelium culture supernatants than in mycelial extracts. Mycelial extracts and culture supernatants inhibited or increased cyclooxygenase-2 transcription activity and NO production. Various extracts and culture supernatants inhibited B16 cell migration and motility, and inhibited HUVEC tube formation. These findings indicate that DongChongXiaCao extracts and products of mycelium could be a useful biological resource for anti-oxidant and anti-cancer purposes.

동충하초 20여종의 균사와 배양 배지를 이용하여 생물학적 활성 효과를 항산화와 항암 실험을 통하여 알아보았다. 항산화 활성 실험 DPPH와 FRAP실험을 실시하였는데 다수의 동충하초 추출물에서 항산화 활성을 확인할 수 있었으며, 시간에 따른 FRAP활성에서는 배양배지에서 균사체 추출물보다 높은 활성을 나타내었다. NOS와 관련성을 확인하기 위하여 NO활성 실험을 한 결과 NO활성은 추출물의 종류에 따라 증가 또는 감소시키는 것을 알 수 있었다. 염증과의 관련성을 확인하기 위하여 Cox-2 promoter활성실험을 한 결과, 종에 따라 활성의 증가 또는 감소는 다양하게 나타났다. Wound healing assay를 이용하여 항암효과를 알아본 결과 많은 종에서 세포의 운동성을 억제하는 효과를 가지는 것으로 나타났다. 또한 혈관생성 억제효과를 알아보기 위하여 HUVECs를 이용하여 tube formation을 확인해 본 결과 다수의 추출물에서 혈관생성을 억제하는 것으로 나타났다.

Keywords

References

  1. Sundaresan, M., Yu, Z.X., Ferrans, V.J., Irani, K. and Finkel, T. (1995) Requirement for generation of $H_{2}O_{2}$ for platelet-derived growth factor signal transduction. Science, 270, 296-299 https://doi.org/10.1126/science.270.5234.296
  2. Del Rio, L.A., Corpas, F.J., Sandalio, L.M., Palma, J.M., Gomez, M. and Barroso, J.B. (2002) Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes. J. Exp. Bot., 53, 1255-1272 https://doi.org/10.1093/jexbot/53.372.1255
  3. Chung-man Ho, J., Zheng, S., Comhair, S.A.A., Farver, C. and Erzurum, S.C. (2001) Differential expression of manganese superoxide dismutase and catalase in lung cancer. Cancer Res., 61, 8578-8585
  4. MacNee, W. (2001) Oxidative stress and lung inflammation in airways disease. Eur. J. Pharmacol., 429, 195-207 https://doi.org/10.1016/S0014-2999(01)01320-6
  5. Matsuyama, T., Ihaku, D., Tanimukai, T., Uyama, O. and Kitada, O. (1993) Superoxide dismutase suppressed asthmatic response with inhibition of manganese superoxide induction in rat lung. Nippon Kyobu Shikkan Gakkai Zasshi., 31, 139-145
  6. Nadeem, A., Chhabra, S.K., Masood, A., and Raj, H.G. (2003) Increased oxidative stress and altered levels of antioxidants in asthma. J. Allergy Clin. Immunol., 111, 72-78 https://doi.org/10.1067/mai.2003.17
  7. Varshavskii, B., Trubnikov, G.V., Galaktipmpva, L.P., Koreniak, N.A., Koledeznaia, I. L. and Oberemok, A.N. (2003) Oxidant-antioxidant status of patients with bronchial asthma during inhalation and systemic glucocorticoid therapy. Ter. Arkh., 75, 21-24
  8. Afonso, V., Champy, R., Mitrovic, D., Collin, P. and Lomri, A. (2007) Reactive oxygen species and superoxide dismutases: role in joint diseases. Joint Bone Spine, 74, 324-329 https://doi.org/10.1016/j.jbspin.2007.02.002
  9. Fang, J., Nakamura, H. and Iyer, A.K. (2007) Tumor-targeted induction of oxystress for cancer therapy. J. Drug Target, 15, 475-486 https://doi.org/10.1080/10611860701498286
  10. Al-Gayyar, M.M., Eissa, L.A., Rabie, A.M. and El-Gayar, A.M. (2007) Measurements of oxidative stress status and antioxidant activity in chronic leukaemia patients. J. Pharm. Pharmacol., 59, 409-417 https://doi.org/10.1211/jpp.59.3.0011
  11. Rao, Y.K., Fang, S.H. and Tzeng, Y.M. (2007) Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. J. Ethnopharmacol., 114, 78-85 https://doi.org/10.1016/j.jep.2007.07.028
  12. Huang, H., Wang, H. and Luo, R.C. (2007) Inhibitory effects of cordyceps extract on growth of colon cancer cells. Zhong Yao Cai., 30, 310-313
  13. Lee, H.M., Kim, Y.J., Kim, H.W., Lee, D.H., Sung, M.K. and Park, T.S. (2006) Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. Biol. Pharm. Bull., 29, 670-674 https://doi.org/10.1248/bpb.29.670
  14. Nomura, T., Kikuchi, M., Kubodera, A. and Kawakami, Y. (1997) Proton-donative antioxidant activity of fucoxanthin with 1,1-diphenyl-2-picrylhydrazyl(DPPH). Biochem Mol. Biol. Int., 42, 361-370
  15. Benzie, I.F. and Strain, J.J. (1996) The ferric reducing ability of plasma(FRAP) as a measure of 'antioxidant power': the FRAP assay. Anal. Biochem., 239, 70-76 https://doi.org/10.1006/abio.1996.0292
  16. Du, C., Guan, Q., Diao, H., Yin, Z. and Jevnikar, A.M. (2006) Nitric oxide induces apoptosis in renal tubular epithelial cells through activation of caspase-8. Am. J. Physiol. Renal Physiol., 290, 1044-1054 https://doi.org/10.1152/ajprenal.00341.2005
  17. Kaltschmidt, B., Linker, R.A., Deng, J. and Kaltschmidt, C. (2002) Cyclooxygenase-2 is a neuronal target gene of NF-kappaB. BMC Mol. Biol., 4, 3-16
  18. Heo, J.C., Park, J.Y., Lee, J.M., Kwon, T.K., Kim, S.U., Chung, S.K. and Lee, S.H. (2005) Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity. J. Ethnopharmacol., 102, 10-14 https://doi.org/10.1016/j.jep.2005.04.036
  19. Bussolati, B., Dunk, C., Grohman M., Kontos, C.D., Mason, J. and Ahmed, A. (2001) Vascular endothelial growth factor receptor-1 modulates vascular endothelial growth factor-mediated angiogenesis via nitric oxide. Am. J. Pathol., 159, 993-1008 https://doi.org/10.1016/S0002-9440(10)61775-0
  20. Lin, X.X., Xie, Q.M., Shen, W.H. and Chen, Y. (2001) Effects of fermented Cordyceps powder on pulmonary function in sensitized guinea pigs and airway inflammation in sensitized rats. Zhongguo Zhong Yao Za Zhi., 26, 622-625
  21. Kuo, Y.C., Tsai, W.J., Wang, J.Y., Chang, S.C., Lin, C.Y. and Shiao, M.S. (2001) Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis. Life Sci., 68, 1067-1082 https://doi.org/10.1016/S0024-3205(00)01011-0
  22. Dreher, D. and Junod, A.F. (1996) Role of oxygen free radicals in cancer development. Eur. J. Cancer, 32A, 30-38
  23. Droge, W. (2002) Free radicals in the physiological control of cell function. Physiol. Rev., 82, 47-95 https://doi.org/10.1152/physrev.00018.2001
  24. Akaike, T., Okamoto, S., Sawa, T., Yoshitake, J., Tamura, F., Ichimori, K., Miyazaki, K., Sasamoto, K. and Maeda, H. (2003) 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis. Proc. Natl. Acad. Sci. U.S.A., 100, 685-690
  25. Fujii, S., Akaike, T. and Maeda, H. (1999) Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats. Virology, 256, 203-212 https://doi.org/10.1006/viro.1999.9610
  26. van Wetering, S., van den Berk, N., van Buul, J.D., Mul, F.P., Lommerse, I., Mous, R., ten Klooster, J.P., Zwaginga, J.J. and Hordijk, P.L. (2003) VCAM-1- mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am. J. Physiol. Cell Physiol., 285, 343-352 https://doi.org/10.1152/ajpcell.00048.2003
  27. Kuwahara, H., Kanazawa, A., Wakamatu, D., Morimura, S., Kida, K., Akaike, T. and Maeda, H. (2004) Antioxidative and antimutagenic activities of 4-vinyl-2,6- dimethoxyphe nol(canolol) isolated from canola oil. J. Agric. Food Chem., 52, 4380-4387 https://doi.org/10.1021/jf040045+
  28. Yoshitake, J., Akaike, T., Akuta, T., Tamura, F., Ogura, T., Esumi, H. and Maeda, H. (2004) Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity. J. Virol., 78, 8709-8719 https://doi.org/10.1128/JVI.78.16.8709-8719.2004
  29. Huang, P., Feng, L., Oldham, E.A., Keating, M.J. and Plunkett, W. (2000) Superoxide dismutase as a target for the selective killing of cancer cells. Nature, 407, 390-395 https://doi.org/10.1038/35030140
  30. Fang, J., Sawa, T. and Maeda, H. (2003a) Factors and mechanism of 'EPR' effect and the enhanced antitumor effects of macromolecular drugs including SMANCS. Adv. Exp. Med. Biol., 519, 29-49
  31. Fang, J., Sawa, T., Akaike, T., Akuta, T., Sahoo, S.K, Greish, K., Hamada, A. and Maeda, H. (2003b) In vivo antitumor activity of pegylated zinc protoporphyrin: Targeted inhibition of heme oxygenase in solid tumor. Cancer Res., 63, 3567-3574