Changes of Bioactive Compounds and Antioxidant Activities in Korean Green Tea (Camellia sinensis) with Different Harvesting Periods

국산 녹차의 채취시기별 활성물질 및 항산화능 변화

  • Kang, Seong-Koo (Department of Food Science and Technology, Sunchon National University) ;
  • Shon, Mi-Yae (Department of Food and Nutrition, Gyeongsang National University)
  • Published : 2007.12.31

Abstract

Korean green tea has been claimed to have health-promoting effects, which may be related to the antioxidant activity in vitro. Korean green teas (Woojeon, WJ ; Sejak, SJ ; Jungjak, JJ ; Daejak, DJ) were subjected to different harvested times and yet little research has examined their bioactive compounds. To assess the effect of this different harvested times on nutritional and health-related properties such as Korean green tea polyphenols, flavonoids, theanine and free amino acids, antioxidant activities and radical scavenging activities were determined. Total polyphenols in JJ (37.16 mg/g) was higher than in other products (WJ, 19.55 ; SJ 24.65 ; DJ, 23.28 mg/g). Contents of flavonol and flavone glycosides were the highest at DJ (350.83 mg%) as compared to WJ (220.81), SJ (256.88) and JJ (270.36 mg%). Contents of theanine and total free amino acids were the highest at WJ (14.11, 23.62 mg/g, respectively), but decreased thereafter. Antioxidant activities were higher in JJ and DJ, using the linoleic acid peroxidation, DPPH and ABTS free radical-scavenging activities. However, WJ and SJ had less active antioxidant activity and free radical-scavenging activity. Reducing powers were increased depend on the concentration of extracts. Antioxidant activity and free radical-scavenging activity of JJ and DJ seemed to relate with total polyphenol and flavonoid contents.

국산 녹차의 채취시기별 주요 화학성분과 항산화 특성을 평가하기 위하여 폴리페놀, 플라보노이드, 데아닌, 유리아미노산 함량과 항산화 및 라디칼 소거능에 대하여 조사하였다. 총 폴리페놀 함량은 중작 37.16 mg/g로 가장 높았으며, 다음으로 세작 24.65 mg/g, 대작 23.28 mg/g, 우전 19.55 mg/g 순이었으며, 총 플라보노이드는 대작이 350.83 mg%으로 중작 270.36 mg%, 세작 256.88 mg%, 우전 220.81 mg%에 비하여 비교적 높았다. Theanine과 총 유리아미노산은 우전에서 각각 14.11 mg/g, 23.62 mg/g으로 가장 높았으며, 이후에 나오는 녹차는 그 함량이 점진적으로 감소되었다. 또한 수확시기에 따른 항산화능을 linoleic acid peroxidation, DPPH 및 ABTS 자유라디칼 소거능으로 조사한 결과, 녹차 중 중작이나 대작에서는 높았지만, 우전과 세작은 항산화 활성과 자유라디칼 소거능이 거의 없었고, 그 환원력은 농도 의존적으로 증가하였다. 이상의 결과로부터 중작과 대작의 항산화능과 자유라디칼 소거능은 총 폴리페놀과 총 플라보노이드 함량에 관련되는 것으로 판단된다.

Keywords

References

  1. Shon, M.Y., Kim, S.H., Nam, S.H., Park, S.K. and Sung, N.J. (2004) Antioxidant activity of Korean green and fermented tea extracts. J. Life Sci., 14, 920-924 https://doi.org/10.5352/JLS.2004.14.6.920
  2. Ohe, T., Marutani, K. and Nakase, S. (2001) Catechins are not major compounds responsible for anti-genotoxic effects of tea extracts against nitroarenes. Mutation Research, 496, 75-81 https://doi.org/10.1016/S1383-5718(01)00220-0
  3. Stensvold, I., Tverdal, A., Solvoll, K. and Foss, O.P. (1992) Tea consumption. Relationship to cholesterol, blood pressure, and coronary and total mortality. Prev. Med., 21, 546-553 https://doi.org/10.1016/0091-7435(92)90062-M
  4. Kono, S., Shinchi, K., Ikeda, N., Yanai, F. and Imanishi, K. (1992) Green tea consumption and serum lipid profiles : A cross sectional study in Northern Kyushu, Japan. Preventive Medicine, 21, 526-531 https://doi.org/10.1016/0091-7435(92)90060-U
  5. Yang, T.T.C. and Koo, K.M.L. (2000) Inhibitory effect of Chinese green tea on endothelial cell induced LDL oxidation. Atheroscleorosis, 148, 67-83 https://doi.org/10.1016/S0021-9150(99)00239-7
  6. Higdon, J.V. and Frei, B. (2003) Tea catechins and polyphenols : Health effects, metabolism and antioxidant functions. Critical Reviews Food Sci. Nutr., 43, 89-143 https://doi.org/10.1080/10408690390826464
  7. Terao, J., Piskula, M. and Yao, Q. (1994) Protective effect of epicatechin, epicatechin gallate, and quercetin on lipid peroxidation in phospholipid bilayers. Archives Biochem. Biophy., 308, 278-284 https://doi.org/10.1006/abbi.1994.1039
  8. Riemersma, R.A., Carruthers, K.F. and Elton, R.A. (2001) Plasma ascorbic acid and risk of heart disease and cancer. Lancet, 357, 2136-2137 https://doi.org/10.1016/S0140-6736(00)05208-9
  9. Barnham, K.J., Masters, C.L. and Bush, A.I. (2004) Neurodegenerative diseases and oxidative stress. Nature Reviews Drug Discovery, 3, 205-214 https://doi.org/10.1038/nrd1330
  10. Cooke, M.S., Evans, M.D., Dizdaroglu, M. and Lunec, J. (2003) Oxidative DNA damage: mechanism, mutation and disease. FASEB J., 17, 1195-1214 https://doi.org/10.1096/fj.02-0752rev
  11. Brash, D.E. and Harve, P.A. (2002) New careers for antioxidants. Proceedings of the National Academy of Sciences of the United States of America, 99, 13969-13971
  12. Surh, Y.J. (2003) Cancer chemoprevention with dietary phytochemicals. Nature Reviews Cancer, 3, 768-780 https://doi.org/10.1038/nrc1189
  13. Hou, Z., Lambert, J.D., Chin, K.V. and Yang, C.S. (2004) Effects of tea polyphenols on signal transduction pathways related to cancer chemoprevention. Mutation Research, 555, 3-19 https://doi.org/10.1016/j.mrfmmm.2004.06.040
  14. Mitscher, L.A., Jung, M., Shankel, D., Dou, J.H., Steele, L. and Pillai, S.P. (1997) Chemoprotection : a review of the potential therapeutic antioxidant properties of green tea (Camellia sinensis) and certain of its constituents. Medicinal Research Reviews, 17, 327-365 https://doi.org/10.1002/(SICI)1098-1128(199707)17:4<327::AID-MED2>3.0.CO;2-Y
  15. Zhou, B., Wu, L.M. and Liu, Z.L. (2005) Evidence for ${\alpha}$-tocopherol regeneration of green tea polyphenols in SDS micelles. Free Radical Biol. Med., 38, 78-84 https://doi.org/10.1016/j.freeradbiomed.2004.09.023
  16. Arabbi, P.R., Genovese, M.I. and Lajolo, F.M. (2004) Flavonoids in vegetable foods commonly consumed in Brazil and estimated ingestion by the Brazilian population. J. Agric. Food Chem., 52, 1124-1131 https://doi.org/10.1021/jf0499525
  17. Wang, H., Provan, G.J. and Helliwell, K. 2003. HPLC determination of catechins in tea leaves and tea extracts using relative respones factors. Food Chemistry, 81, 307-312 https://doi.org/10.1016/S0308-8146(02)00510-1
  18. Yoshida, T.K., Mori, T., Okumura, I., Uehara, K. Komagoe, Y. and Fuijita, T. (1989) Studies on inhibition mechanism of autoxidation by tannins and flavonoids. Chem. Pharm. Bull., 37, 1919-1921 https://doi.org/10.1248/cpb.37.1919
  19. Re, R., Pellegrini, N., Pannala, A., Yang, M. and Rice-Evans, C. (1999) Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biol. Med., 26, 1231-1237 https://doi.org/10.1016/S0891-5849(98)00315-3
  20. Mitsuda, H., Yasumoto, K. and Iwami, K. (1996) Antioxidative action of indole compounds during the autoxidation of linoleic acid. Eiyo to Shokur, 19, 210-214
  21. Yen, G.C. and Duh, P.D. (1993) Antioxidative properties of methanolic extracts from peanut hulls. J. American Oil Chemist's Society, 70, 383-386 https://doi.org/10.1007/BF02552711
  22. Shahidi, F. and Wanasundara, P.K. (1992) Phenolic antioxidants. Critical Review Food Sci. Nutr., 32, 67-103 https://doi.org/10.1080/10408399209527581
  23. Nakama, T., Yamada, M., Osawa, T. and Kawakishi, S. (1993) Suppression of active oxygen-induced cytotoxicity of flavonoids. Biochem. Pharmacol., 45, 265-267 https://doi.org/10.1016/0006-2952(93)90402-I
  24. Pietta, P. and Simonetti, P. (1999) Dietary flavonoids and interaction with physiologic antioxidants. In: Packer, L., Hiramatsu, M., Yoshikawa, T. : Antioxidant food supplements in human health. Academic Press, San Diego, California. p.283-308