Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Inhibition of LPS Induced iNOS, COX-2 and Cytokines Expression by $Genistein-4'-O-{\alpha}-L-Rhamnopyranosyl-(1-2)-{\beta}-D-Glucopyranoside$ through the $NF-{\kappa}B$ Inactivation in RAW 264.7 Cells

$Genistein-4'-O-{\alpha}-L-rhamnopyranosyl-(1-2)-{\beta}-D-glucopyranoside$의 RAW 264.7 세포에서 $NF-{\kappa}B$ 불활성화를 통한 LPS에 의해 유도되는 iNOS, COX-2 그리고 cytokine들의 발현 저해효과

  • Park, Seung-Jae (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Kim, Ji-Yeon (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Jang, Young-Pyo (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University) ;
  • Cho, Young-Wuk (Department of Physiology, College of Medicin,e Kyung-Hee University) ;
  • Ahn, Eun-Mi (Department of Herbal Food Science, Daegu Haany University) ;
  • Baek, Nam-In (Department of Life Science, Kyung-Hee University) ;
  • Lee, Kyung-Tae (Department of pharmaceutical Biochemistry, College of Pharmacy, Kyung-Hee University)
  • Published : 2007.12.31
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Abstract

This study were designed to evaluate the anti-inflammatory effects of $genistein-4'-O-{\alpha}-L-rhamnopyranosyl-(1-2)-{\beta}-D-glucopyranoside$ (GRG) isolated from Sophora japonica (Leguminosae) on the lipopolysaccharide (LPS)-induced nitric oxide (NO) and prostaglandin ($PGE_2$) production by RAW 264.7 cell line. GRG significantly inhibited the LPS-induced NO and $PGE_2$ production. Consistent with these observations, GRG reduced the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the protein and mRNA levels in a concentration-dependent manner. In addition, the release and the mRNA expression levels of tumor necrosis $factor-{\alpha}\;(TNF-{\alpha})$ and interleukin-6 (IL-6) were also reduced by GRG. Moreover, GRG attenuated the LPS-induced activation of nuclear factor-kappa B ($NF-{\kappa}B$), a transcription factor necessary for pro-inflammatory mediators, iNOS, COX-2, $TNF-{\alpha}$ and IL-6 expression. These results suggest that the down regulation of iNOS, COX-2, $TNF-{\alpha}$, and IL-6 expression by GRG are achieved by the downregulation of $NF-{\kappa}B$ activity, and that is also responsible for its anti-inflammatory effects.

Keywords

References

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