Anti-inflammatory Effect of Scutellariae Radix

  • Lee, Eun (Department of Animal Biotechnology and Resources, Sangji University)
  • Published : 2007.12.30

Abstract

This research is the basic research to develop new anti-inflammatory medicine by feeding Scutellariae Radix extract to lipopolysaccharide(LPS) exposed rats, and analyzed it's effect on inflammatory response by LPS derivation. As a result, Plasma interleukin-$1\beta(IL-1\beta)$ and Plasma interleukin-6(IL-6) concentration showed the highest point at 5h after LPS injection, and in this time, the concentration of $IL-1\beta$ and IL-6 in the Scutellariae Radix extract groups at 200mg/kg and 300mg/kg showed lower values than that of control group. Plasma tumor necrosis $factor-\alpha(TNF-\alpha)$ concentration after LPS injection showed the highest point at 2h and showed similar level till at 5h. $TNF-\alpha$ concentration at 2h after LPS injection showed the low value only in the Scutellariae Radix extract 300mg/kg group compared to others, and in 5h, the all Scutellariae Radix extract groups showed lower value than that of the control group. Plasma interleukin-10(IL-10) concentration increased at 2h after LPS injection and reached the highest at 5h. After LPS injection the IL-10 concentration at 2h, the Scutellariae Radix extract injection group at 300mg/kg showed higher value than that of the others, and in 5h after LPS injection, Scutellariae Radix extract 200mg and 300mg groups showed higher value than the control group. Concluding from the above results, in inflammatory response by LPS derivation, the Scutellariae Radix gives positive effect.

Keywords

References

  1. Aono, K., K. Isobe, K. Kuichi, Z. Fan, M. Ito and A. Takeuchi.1997. In vitro and in vivo expression of inducible nitric oxide synthase during experimental endotoxemia: involvement of other cytokines. J. cell Biochem. 65: 349-58 https://doi.org/10.1002/(SICI)1097-4644(19970601)65:3<349::AID-JCB5>3.0.CO;2-S
  2. Barton, C.C., E.X. Barton, P.E. Ganey, S.L. Kunkel and R.A. Roth. 2001. Bacterial lipopolysaccharide enhances aflatoxin Bi hepatotoxicity in rats by a mechanism that depends on tumor necrosis factor-$\alpha$. Hepatology 33: 66-73 https://doi.org/10.1053/jhep.2001.20643
  3. Boumpas, D.T., G.P. Chrousos and R.L. Wilder. 1993. Glucocorticoid therapy for immune mediated disease: Casic and clinical correlates. Ann. Intern. Med. 119: 1198-1208 https://doi.org/10.7326/0003-4819-119-12-199312150-00007
  4. Chamulitrat, W., M.E. Blazka, S.J. Jordan, M.l. Luster and R.P. Mason. 1995. Tumor necrosis factor-$\alpha$ and nitric oxide production in endotoxin-primed rats administered carbon tetrachloride. Life Sci. 24: 2273-80
  5. Corral, L.G., G.W. Muller, A.L. Moreira, X. Chen, M. Wu and D. Stirling. 1996. Selection of novel analogs of thalidomide with enhanced tumor necrosis factor-$\alpha$ inhibitory activity. Mol. Med. 25: 964-9
  6. Harbrecht, B.G., M. DiSilvio, A.J. Demetris, R.L. Simmons and T.R. Billiar. 1994. Tumor necrosis factor-$\alpha$ regulates in vivo nitric oxide synthesis and induces liver injury during endotoxemia. Hepatology 20: 1055-60 https://doi.org/10.1002/hep.1840200439
  7. Harry, D., R. Anand, S. Holt, S. Davies, R. Marley and B. Fernando. 1999. Increased sensitivity to endotoxemia in the bile duct-ligated cirrhotic rat. Hepatology 30: 1198-205 https://doi.org/10.1002/hep.510300515
  8. Hamada, E., T. Nishida, Y. Uchiyama, J. Nakamura, K. Isihara and H. Kazuo, 1999. Activation of Kupffer cells and caspases- 3 involved in rat hepatocyte apoptosis induced by endotoxin. J. Hepatol. 30: 807-18 https://doi.org/10.1016/S0168-8278(99)80133-0
  9. Kim, H. C. 2001. Textbook of herbal pharmacology. Jipmundang. 129-133
  10. Kubo, M., H. Matusuda, Y. Kimura, H. Okuda, M. Higashino, T. Tani, K. Namba and S.Arichi. 1984. Stidies on scutellariae radix. VII. Anti-arthrictic and anti-inflammatory action of methanolic extract and flavonoid components from Scutellariae Radix. Chem. Pharm. Bull. 33: 2411-2415
  11. Kubo, M., Y. Kimura, T. Odani, T. Tani andK. Namba. 1981. Studies on scutellariae radix.II., The antibacterial substance. Planta. Med. 43: 194-201 https://doi.org/10.1055/s-2007-971499
  12. Lee, S.H., B.O. Lim and R.W. Choue. 2004. Immunoregulatory effects of water extracts of Scutellariae radix in DSS-induced inflammatory bowel disease animal model. Korean Nutr. 37: 431-439
  13. Marriot, J.B., M. Westby, S. Cookson, M. Guckian, S. Goodboum and G. Muller. 1998. CC-3052: a water-soluble analog of thalidomide and potent inhibitor of activation-induced TNF-$\alpha$ production. J. Immunol. 161: 4236-43
  14. Mathiak, G., G. Grass, T. Herzmann, T. Luebke, C. Cu-Zetina and S.A Boehm. 2000. Capase-1- inhibitor ac-YVAD-cmk reduces LPS-lethality in rats without affecting haematology or cytokine responses. Br J. Pharmacol. 131: 383-6 https://doi.org/10.1038/sj.bjp.0703629
  15. Middleton, Jr.E. and V. Drzewiecki. 1985. Naturally occuring flavonoids and human basophil histamine release. Int. Archs. Allergy Appl. Immun. 77: 155-157 https://doi.org/10.1159/000233771
  16. Middleton, Jr.E., J.B. Harborne and A. Beretz. 1988. Plant flavonoids in biology and medicine II. Biochemical, cellular and medicineal properties. Alan R. Liss, New York 61-65
  17. Middleton, Jr.E. and J.B. Harborne (des.). 1986. Plant flavonoids in biology and medicine, biochemical, pharmacological, structureactivity relationship. Alan R. Liss, New York 429-440
  18. Moreira, A.L., E.P. Sampaio, A. Zmuidzinas, P. Frindt, K.A. Smith and G. Kaplan. 1993. Thalidomide exerts its inhibitory action on tumor necrosis factor alpha by enhancing mRNA degradation. J. Exp. Med. 177: 1675-80 https://doi.org/10.1084/jem.177.6.1675
  19. Sang, H., G.L. Wallis, C.A. Stewart and K. Yashige. 1999. Expression of cytokines and activation of transcription factors in lipopolysaccharide-administered rats and their inhibition by phenyl N-tert-butylnitrone (PBN). Arch. Biochem. Biophys. 363: 341-8 https://doi.org/10.1006/abbi.1998.1086
  20. Tie, H., G.B. Jin, G.B. Cho, S. Fumi and J.C. Cyong I. 2002. Evaluation of the anti-inflammatory effect ofbaicalein on dextran sulfate sodium-induced colitis in mice. Planta. Med. 68: 266-268 https://doi.org/10.1055/s-2002-23126
  21. Tompson, K.C., A. Trowem, A. Fowell, M. Marathe, C. Haycock and M.J.P. Arthur et al. 1998. Primary rat and mouse hepatic stellate cells express the macrophage inhibitor cytokine interleukin-10 during the course of activation in vitro. Hepatology 28: 1518-1524 https://doi.org/10.1002/hep.510280611
  22. Wagner, H. 1986. Antihepatotoxic flavonoids, In Cody, Middleton Jr. E, Harborne (des) JB, Plant Flavonoids in Biology and Medicine, Biochemical, Pharmacological and Structure-Activity Relationships. Alan R. Liss, New York 545-558