Trans-Resveratrol Contents of Peanut Seeds Depend on Varieties and Processing Methods

  • Published : 2007.12.30

Abstract

The high-performance liquid chromatography(HPLC) method for the determination of trans-resveratrol in 34 germplasms and processing methods of peanut seeds has been modified. Peanut germplasms contained trans-resveratrol contents of $0.14{\sim}4.96{\mu}g/g$, but findings for the testa color were not significant. However, two germplasms, 'KIGAN' and 'CS1', contained more trans-resveratrol contents than the other germplasms. The contents of their were $2.26{\mu}g/g\;and\;4.96{\mu}g/g$. The tested processing methods caused no significant changes in trans-resveratrol contents. The contents of fresh, boiled, and roasted peanuts were 0.36, 0.32, and $0.40{\mu}g/g$, respectively in cv. Palkwang, and 0.22, 0.22, and $0.26{\mu}g/g$, respectively, in cv. Jakwang. Differences were not significant among fresh, boiled, and roasted peanuts. The grains of 'Palkwang' and 'Jakwang' contained trans-resveratrol contents of $0.34{\mu}g/g\;and\;0.24{\mu}g/g$, and testa contained $1.12{\mu}g/g\;and\;1.00{\mu}g/g$, respectively. However, when comparing absolute quantity, the trans-resveratrol contents appears to be approximately $3{\sim}4$ times higher in the testa than in the grain of the peanut, although the total contents were not different because the ratio of testa was low in peanut seeds.

Keywords

References

  1. Arora, M. K. and R. N. Strange. 1991. Phytoalexin accumulation in groundnuts in response to wounding. Plant Sci. 78: 157-163 https://doi.org/10.1016/0168-9452(91)90194-D
  2. Chan, M. M. 2002. Antimicrobial effect of resveratrol on dermatophytes and bacterial pathogens of the skin. Biochem. Pharmacol. 63: 99-104 https://doi.org/10.1016/S0006-2952(01)00886-3
  3. Cho, G. S. 1993. Chemical Composition in the various plant types of peanut (Arachis hypogaea L.). J. Korean Soc. Food Nutr. 22(5): 576-580
  4. Chung, I. M., M. R. Park, S. Rehman and S. J. Yun. 2001. Tissue specific and inducible expression of resveratrol synthase gene in peanut plants. Molecules and Cells 12(3): 353-359
  5. Duh, P. D. and G. C. Yen. 1995. Changes in antioxidant activity and components of methanolic extracts of peanut hulls irradiated with ultraviolet light. Food Chemistry 54(2): 127-131 https://doi.org/10.1016/0308-8146(94)00148-X
  6. Duh, P. D., D. B. Yen and G. C. Yen. 1992. Extraction and identification of an antioxidative component from peanut hulls. Journal of American Oil Chemistry Society 69: 814-818 https://doi.org/10.1007/BF02635922
  7. Fritzemeier, K. H., C. H. Rolfs, J. Pfau and H. Kindl. 1983. Action of ultraviolet-C on stilbene formation in callus of Arachis hypogaea. Planta 159: 25-29 https://doi.org/10.1007/BF00998810
  8. Ingham, J. L. 1976. 3, 5 ,6'-Trihydroxystilbene as a phytoalexin from ground nuts (A. hypogaea). Phytochemistry 15: 1791-1793 https://doi.org/10.1016/S0031-9422(00)97494-6
  9. Jang, M., L. Cai, G. O. Udeani, K. V. Slowing, C. F. Thomas, C. W. W. Beecher, H. H. S. Fong, N. R. Farnsworth, A. D. Kinghorn, R. G. Mehta, R. C. Moon and J. M. Pezzuto. 1997. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275: 218-220 https://doi.org/10.1126/science.275.5297.218
  10. Jeandet, P., R. Bessis, B. F. Maume, P. Meunier, D. Peyron and P. Trollat. 1995. Effects of enological practices on the resveratrol isomer content of wine. J. Agric. Food Chem. 41: 521-523 https://doi.org/10.1021/jf00028a001
  11. Kashiwada, Y., G. Nonaka, I. Nishioka, M, Nishixawa and T. Yamagishi. 1998. Studies on rhubarb (Rhei rhizoma). XIV. Stilbene glucosides. Chem. Pharm. Bull. 36: 1545-1549
  12. Keen, N. T. 1975. The isolation of phytoalexins from germinating seeds of Cicer arietinum, Bigna sinensis, Arachis hypogaea and other plants. Phytopathology 65: 91-92 https://doi.org/10.1094/Phyto-65-91
  13. Keen, N. T. and J. L. Ingham. 1976. New stilbene phytoalexins from American cuitivars of A. hypogaea. Phytochemistry 15: 1794-1795 https://doi.org/10.1016/S0031-9422(00)97495-8
  14. Langcake, P. 1981. Disease resistance of Vitis spp, and the production of the stress metabolites resveratrol, $\alpha$-viniferin, aviniferin and pterostilbene. Physiol. Plant Pathol. 18: 213-216 https://doi.org/10.1016/S0048-4059(81)80043-4
  15. Lanz, T., G. Schroder and J. Schroder. 1990. Differential regulation of genes for resveratrol synthase in cell cultures of Arachis hypogaea L. Planta 181: 169-175 https://doi.org/10.1007/BF02411534
  16. Lee, M. J., Y. K. Cheong, H. S. Kim, K. H. Park, H. S Doo and D. Y. Suh. 2003. trans-Resveratrol content of varieties and growth period in peanut. Kor. J. Crop Sci. 48(6): 429-433
  17. Lotan, R., E. Skutelsky, D. Danon and N. Sharon. 1975. The purification, composition and specificity of the anti- T lection form peanut (Arachis hypogaea), Journal of Biology Chemistry 250: 8518-8523
  18. Mattivi, F., F. Reniero and S. Korhammer. 1995. Isolation, characterization, and evolution in red wine vinification of resveratrol monomers. J. Agric. Food Chem. 43: 1820-1823 https://doi.org/10.1021/jf00055a013
  19. Park, M. R., S. H. Baek, Y. K. Cheong. M. H. Lee, K. G. Choi and S. J. Yun. 2001. Tocopherol content and composition in peanut. Korean J. Crop Sci. 46(4): 279-283
  20. Raiber, S., G. Schrder and J. Schrder. 1995. Molecular and enzymatic characterization of two stilbene synthases from Eastern white pine (Pinus strobus). A single Agr/His difference determines the activity and the pH dependence of the enzymes. FEBS Lett. 361: 299-302 https://doi.org/10.1016/0014-5793(95)00199-J
  21. Romero-perez, A. I., R. M. Lamuela-Raventos, C. Andres-Lacueva, A. L. Cristina and M. C. de la Torre-Boronat. 2001. Method for the quantitative extraction of resveratrol and piceid isomers in grape berry skins. Effect of powdery mildew on the stilbene content. J. Agric. Food Chem. 49: 210-215 https://doi.org/10.1021/jf000745o
  22. Romero-Perez, A. I., R. M. Lamuela-Ravents, R. Buxaderas and M. C. de la Torre-Boronat. 1996. Resveratrol and piceid as varietal markers of white wines. J. Agric. Food Chem. 44: 1975-1978 https://doi.org/10.1021/jf960211g
  23. Sanders, T. H., R. W. McMchael and K. W. Hendrix. 2000. Occurrence of resveratrol in edible peanuts. J. Agric. Food Chem. 48: 1243-1246 https://doi.org/10.1021/jf990737b
  24. Schanz, S., G. Schroder and J. Schroder. 1992. Stilbene synthase from Scots pine (Pinus sylvestris). FEBS Lett. 313: 71-74 https://doi.org/10.1016/0014-5793(92)81187-Q
  25. Schoppner, A. and H. Kindl. 1984. Purification and properties of a stilbene synthase from induced cell suspension cultures of peanut. J. BioI. Chem. 263: 6806-6811
  26. Siemann, E. H. and L. I. Creasy. 1992. Concentration of the phytoalexin reveratrol in wine. Am. J. Enol. Vitic. 43: 49-52
  27. Sobolev V. S. 2001. Vanillin content in boiled peanuts. J. of Agric. Food Chem. 49: 3725-3727 https://doi.org/10.1021/jf010118e
  28. Sobolev V. S. and R. J. Cole. 1999. trans-Resveratrol content in commercial peanuts and peanut products. J. Agric. Food Chem. 47: 1435-1439 https://doi.org/10.1021/jf9809885
  29. Sparvoli, F., C. Martin, A. Scienza, G. Gavazzi and C. Tonelli. 1994. Colining and molecular analysis of structural genes involved in flavonoid and stilbene biosynthesis in grape (Vilis vinifera L.). Planat Mol. BioI. 24: 743-745 https://doi.org/10.1007/BF00029856
  30. Wee, J. H. and K. H. Park. 2000. Identification of 3-methoxy-4-hydroxybenzoic acid and 4-hydrocybenzoic acid with antioxidative and antimicrobial activity from Arachis hypogaea shell. Korean J. Biotechnol. Bioeng. 15(5):464-468