Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Dual priming oligonucleotide system for the multiplex detection of tuberculosis in Hanwoo

  • Shin, Jong-Bong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Park, Nam-Yong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Yong-Hwan (Department of Veterinary Research, Gwangju City Institute of Health and Environment) ;
  • Cho, Ho-Seong (Department of Veterinary Pathology, College of Veterinary Medicine, Chonnam National University)
  • Published : 2007.12.30
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Abstract

In present study, we described the reliability of the dual priming oligonucleotide (DPO) multiplex polymerase chain reaction (PCR) for the detection of Mycobacterium tuberculosis complex (MTC) and non-Mycobacterium tuberculosis (NMT) in blood samples of the Korea native cattle, Hanwoo. Among 340 samples 22 (6.5%) were positive in using DPO multiplex PCR, 21 (6.2%) were positive in PCR. The relative agreement between 2 tests was 99.7%, and the agreement quotient (kappa), was 0.95 (excellent). In these results, we demonstrated the successful application of DPO multiplex PCR for the diagnosis of bovine tuberculosis in Hanwoo. Multiplex PCR, using DPO primers, can be useful for the simple diagnosis of bovine tuberculosis in bovine blood samples.

Keywords

References

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