Relationship between Stress Gene Polymorphisms and Litter Size by AI in Pigs

  • Jin, H.J. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, I.C. (Swine Science Division, NIAS, RDA) ;
  • Wee, M.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Yeon, S.H. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Kim, C.D. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Lee, S.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Cho, C.Y. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Cho, S.R. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Son, D.S. (Animal Genetic Resources Station, National Institute of Animal Science, RDA) ;
  • Park, C.K. (College of Animal Resource Science, Kangwon National University) ;
  • Li, Z.D. (College of Biological Science, China Agricultural University)
  • 발행 : 2007.12.31

초록

This study was performed to investigate the relationship between PSS-HSP70 gene polymorphism and artificial insemination (AI) reproductivity in the pigs. The RFLP polymorphism of PSS and the SSCP polymorphisms of HSP70 K1, K3 and K4 PCR product were detected different patterns. In the experiment for AI of fresh semen, spring and fall season showed higher litter size born of 10.89 head than 10.47 head of summer season. Landrace was showed higher litter size of 9.96 head than that of Duroc and Yorkshire (p<0.05). Stress relating PSS and HSP70 polymorphism of PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showd a highest litter size born of 10.97 head and litter size born alive of 10.69 head than that of the other polymorphisms(p<0.05). In the experiment for AI of frozen semen, effects of season and pig breeds were not showed for litter size born. The stress relating polymorphism of PSS-Carrier, HSP70 K1-BB, K3-BB, K4-AB showed highest litter size born of 11.29 head and litter size born alive of 10.82 head and PSS-Normal, HSP70 K1-BB, K3-AB, K4-AA showed the lowest litter size born of 8.48 head and litter size born alive of 7.33 head than that of the other polymorphisms(p<0.05). These results suggest that AI litter size born for the stress of forzen thawed semen may be affected by PSS and HSP70 polymorphism in pigs.

키워드

참고문헌

  1. Cheng SP and Wung SC. 1974. A study of seasonal changes in the boar semen characteristics. Research Report Animal Industry Research Institute, Taiwan Sugar Corporation, pp. 89-103 (In Chinese, with English abstract)
  2. Chowdbury AK and Steinberger E. 1964. A quantitative study of the effect of heat on germinal epithelium of rat testes. Am. J. Anat., 115: 509-524 https://doi.org/10.1002/aja.1001150307
  3. Christenson RK, Teaque HS, Grifo Jr. AP and Roller WL. 1972. The effect of high environmental temperature on the boar. Ohio Swine Research and Information Report, Ohio Agricultural Research and Development Center, Wooster, OH, USA, pp. 19-23
  4. Craig EA and Gross CA. 1991. Is HSP70 the cellular thermometer? TIBS., 16: 135-140 https://doi.org/10.1016/0968-0004(91)90055-Z
  5. Fujii JK, Otsu F, Zorzato S, De Leon VK, Khanna JE, Weiler PJ, Brien O and Maclennan DH. 1991. Identification of a mutation in porcine ryanodine receptor associated with malignant hyperthermia. Science, 253: 448-451 https://doi.org/10.1126/science.1862346
  6. Huang SY, Kuo YH, Lee YP, Tsou HL, Lin EC, Ju CC and Lee WC. 2000. Association of heat shock protein 70 with semen quality in boars. Anim. Reprod. Sci., 63: 231-240 https://doi.org/10.1016/S0378-4320(00)00175-5
  7. Jin HJ, Kim IC, Wee MS, Yeon SH, Kim CD, Cho CY, Choi SH, Cho SR, Son DS, Kim YK, Jung JR, Choi HS, Park CK and Kim CI. 2005. Relationship between HSP70 gene Polymorphisnms and IVF embryo development in pigs. Korean J. Emb. Trans., 20(3):289-295
  8. Jin HJ, Park BY, Park JC, Hwang IH, Lee SS, Yeon SH, Kim CD, Cho CY, Kim YK, Min KS, Feng ST, Li ZD, Park CK and Kim CI. 2006. The effects of stress related genes on carcass traits and meat quality in pigs. Asian-Aust. J. Anim. Sci., 19(2):280-285
  9. Kennedy BW and Wilkins IN. 1984. Boar, breed and environmental factors influencing semen characteristics of boars used in artificial insemination. Can. J. Anim. Sci. 64: 833-843 https://doi.org/10.4141/cjas84-097
  10. Koh TJ, Crabo BG, Tsou HL and Graham EF. 1976. Fertility of liquid boar semen as influenced by breed and season. J. Anim. Sci., 42: 138-144 https://doi.org/10.2527/jas1976.421138x
  11. Kuo YH, Huang SY and Lee YP. 1997. Effects of breed and season on semen characteristics of boars in subtropical area. J. Chin. Soc. Vet. Sci. 23: 114-122 (In Chinese, with English abstract)
  12. Leung SM, Senisterra G, Ritchie KP, Sadis SE, Lepock JR and Hightower LE. 1996. Thermal activation of the bovine HSC70 molecular chaperone at physiological temperatures: physical evidence of a molecular thermometer. Cell Stress Chaperones, 1: 78-89 https://doi.org/10.1379/1466-1268(1996)001<0078:TAOTBH>2.3.CO;2
  13. Li GC and Laszlo A. 1985. Thermotolerance in Mammalian Cells: A possible role for heat shock protein. In: Atkinson BG, Walden DB. (Eds.), Changes in Eukaryotic Gene Expression in Response to Environmental Stress. Academic Press, Orlando, FL, USA, pp. 227-254
  14. Lindquist S. 1986. The heat-shock response. Annu. Rev. Biochem., 55: 1151-1191 https://doi.org/10.1146/annurev.bi.55.070186.005443
  15. Lindquist S and Craig EA. 1988. The heat-shock proteins. Annu. Rev. Genet., 22: 631-677 https://doi.org/10.1146/annurev.ge.22.120188.003215
  16. Liu SH, Kuo YH, Yang TS and Lee KH. 1994. Relationship between characteristics of serum reproductive hormones and reproductive performance in Yorkshire boars during hot summer months. J. Chin. Soc. Anim. Sci. 23: 33-42 (In Chinese, with English abstract)
  17. Malmgren L and Larsson K. 1984. Semen quality and fertility after heat stress in boars. Acta. Vet. Scand., 25: 425-435
  18. Nover L. 1991. HSFs and HSPs-a stressful program on transcription factors and chaperones. Stress Proteins and the Heat Shock Response, sponsored by Cold Spring Harbor Laboratory, Cold Spring Harbor, NY USA, April 29-May 2, 1991. New Biol., 3(9): 855-859
  19. Pena FJ, Dominguez JC, Carbajo M, Anel L and Alegre B, 1998. Treatment of swine summer infertility syndrome by means of oxytocin under field conditions. Theriogenology, 49(4):829-836 https://doi.org/10.1016/S0093-691X(98)00032-6
  20. Richter LE, Romeny KF, Weitze KF and Zimmermann F. 1975. Zur Tiefgefrierung von Ebersperma. 7 Mitteilung: Weitere Labor-und Besamungsversuhe mit dem Verdunner Hulsenberg Ⅶ. Dtsch Tierarztl. Wschr., 82(4): 155-162
  21. Rokutan K. 2000. Gastric mucosal protection and cell proliferation-role of heat shock proteins in gastric mucosal protection. Journal of Gastroenterology and Hepatology, 15: 12-19 https://doi.org/10.1046/j.1440-1746.2000.02144.x
  22. Rothschild MF and Ruvinsky A. 1998. The genetics of the pig. CAB. INTERNATIONAL, pp. 135-197
  23. Sarnbrook J, Fritsh EF and Maniatis T. 1989. Molecular Cloning: A Laboratory Manual (2nd Ed.). Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
  24. Sanchez Y and Lindquist SL. 1990. HSPI04 is required for induced thermotolerance. Science, 248: 1112-1115 https://doi.org/10.1126/science.2188365
  25. Sanchez Y, Taulien J, Borkovich KA and Lindquist SL. 1992. HSPI04 is required for tolerance to many forms of stress. EMBO J., 11: 2357-2364
  26. Sarge KD. 1995. Male germ cell-specific alteration in temperature set point of the cellular stress response. J. Biol. Chem., 270: 18745-18748 https://doi.org/10.1074/jbc.270.32.18745
  27. SAS Institute. 1989. SAS User's Guide: Statistics. Release 6.3 ed. SAS Institute Inc., Cary, NC, USA
  28. Shichijo K, Ihara M, Matsuu M, Ito M, Okumura Y and Sekine I. 2003. Overexpression of heat hsock protein 70 in stomach of stress-induced gastric ulcer-resistant rats. Digestive Disease and Science, 48: 340-348 https://doi.org/10.1023/A:1021939829515
  29. Shoichi S, Kaoru N, Makoto U and Hiroshi D. 1999. An additional exon of stress-inducible heat shock protein 70 Gene (HSP70-1). Biochemical and Biophysical Research Communications, 257: 193-198 https://doi.org/10.1006/bbrc.1999.0433
  30. Subjeck JR and Shyy TT. 1986. Stress protein systems of mammalian cells. Am. J. Physiol., 250 (1 Pt 1): C1-17 https://doi.org/10.1152/ajpcell.1986.250.1.C1
  31. Tsukimi Y and Okabe S. 2001. Recent advances in gastrointestinal pathophysiology: role of heat shock proteins in mucosal defense and ulcer healing. Biological and Pharmaceutical Bulletin, 24: 1-9 https://doi.org/10.1248/bpb.24.1
  32. Vandemark NL and Free MJ. 1970. Temperature effects. In: Johnson AD, Gomes WR, Vandemark NL. (Eds.), The Testes, Vol. 3. Academic Press, New York, USA, pp. 233-312
  33. Welch WJ. 1992. Mammalian stress response: cell physiology, structure/function of stress proteins and implications for medicine and disease. Physiol. Rev., 72: 1063-1081 https://doi.org/10.1152/physrev.1992.72.4.1063
  34. Wertemann RP and Desjardins C. 1979. Testicular function in boars exposed to elevated ambient temperature. Biol, Reprod., 20: 235-241 https://doi.org/10.1095/biolreprod20.2.235
  35. Wertemann RP, Wells ME, Omtvedt IT, Pope CE and Turman EJ. 1976. Influence of elevated ambient temperature on reproductive performance of boars. J. Anim. Sci., 42: 664-669 https://doi.org/10.2527/jas1976.423664x