Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Effects of ANTORIN R-10 on Ovarian Morphology, Follicular Development and Serum Estradiol Level in Immature Wistar Rats

미성숙 Wistar 랫트에서 ANTORIN R-10이 난소형태, 난포발육 및 혈중 호르몬 농도에 미치는 영향

  • Kang, Eun-Ju (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Yoo, Jae-Gyu (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Song, Hye-Jin (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Song, Seung-Hee (Department of Pet Management, Chanwon college) ;
  • Rho, Gyu-Jin (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University) ;
  • Choe, Sang-Yong (Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University)
  • 강은주 (경상대학교 수의과대학, 동물의학연구소) ;
  • 류재규 (경상대학교 수의과대학, 동물의학연구소) ;
  • 송혜진 (경상대학교 수의과대학, 동물의학연구소) ;
  • 송승희 (창원전문대학 애완동물관리과) ;
  • 노규진 (경상대학교 수의과대학, 동물의학연구소) ;
  • 최상용 (경상대학교 수의과대학, 동물의학연구소)
  • Published : 2007.12.31
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Abstract

This study evaluated the effect of ANTORIN R-10(pFSH), a commercially available follicle stimulating hormone on ovarian morphology, on follicular development and serum estradiol levels in rats. Immature female Wistar S/T rats(27 day old; 80-100 g B.wt) maintained under controlled environmental conditions($22{\pm}2^{\circ}C$; 50% humidity; 12 h light/12 h dark cycle) with free access to standard laboratory feed and tap water were utilized. Animals were allowed to acclimatize to the new environment for at least 2 weeks before being included in the experiment. Rats were randomly allotted to 5 groups(Control, SL 0.1AU, SH 0.2AU, TL 0.1AU and TH 0.2AU). ANTORIN R-10 was subcutaneously injected twice daily for 3 days. Twenty hours after hormone treatment, blood was collected to estimate the serum estradiol $17-\beta$ concentration. Immediately, all rats were sacrificed and the ovarian morphology, ovary weight and number of follicles were recorded. Ovaries were fixed for histomorphological examination. Higher standard and treatment groups were significantly increased on ovary weight and the number of follicles more than 1mm compared with lower standard and treatment. However, no difference revealed between standard and treatment groups. ANTORIN R-10 was similar effects of follicles development and maturation compared with House standard FSH.

Keywords

References

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