The Korean Journal of Pesticide Science (농약과학회지)

The Korean Society of Pesticide Science (한국농약과학회)
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Characterization of Biopesticides (Bacillus thuringiensis) Produced in Korea

국내에서 생산된 Bacillus thuringiensis 살충제의 특성

  • Kil, Mi-Ra (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Da-A (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Choi, Su-Yeon (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Paek, Seung-Kyoung (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, Jin-Su (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Jin, Da-Yong (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Hwang, In-Chon (Central Research Institute, Kyung Nong Co.) ;
  • Yu, Yong-Man (Dept. Applied Biology, College of Agriculture and Life Sciences, Chungnam National University)
  • 길미라 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김다아 (충남대학교 농업생명과학대학 응용생물과) ;
  • 최수연 (충남대학교 농업생명과학대학 응용생물과) ;
  • 백승경 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김진수 (충남대학교 농업생명과학대학 응용생물과) ;
  • 김대용 (충남대학교 농업생명과학대학 응용생물과) ;
  • 황인천 ((주)경농 중앙연구소) ;
  • 유용만 (충남대학교 농업생명과학대학 응용생물과)
  • Published : 2007.09.30
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Abstract

Characteristics of the 5 biopesticides that included Bacillus thuringiensis and on the domestic markets were investigated. These products were contained different strains of B. thuringiensis, for examples; product A and E was B. thuringiensis subsp aizawai; product B was B. thuringiensis; product C was B. thuringiensis Berline var. kurstaki; product D was B. thuringiensis var. kurstaki. Number of active spores were counted because they could influence the bio-activity against target pests. Only product C are contained the fixed quantity as its label, however, product D and E were a tenth part, and product A and B were a hundredth part of their descriptions. The pHs of product A and B were measured 3.67 and 3.73, and C, D and E were 5, respectively. Typical bypyramidal crystals produced from B. thuringiensis was found in only product C under a phase contrast microscope. For the uniform formulation of products that conformed whether B. thuringiensis were equally spreaded on the crops, B. thuringiensis in the C, D and E were equally grown on the nutrient agar medium As a results, product A were more different from product C than any other products. When product A and C were bioassayed against different larval stages of diamondback moth, their mortalities with spraying application were showed 100% after 48 hours.

국내에서 시판되고 있는 5개의 미생물농약(A제품 : Bacillus thuringiensis subsp aizawai, B제품 : B. thuringiensis, C제품 : B. thuringiensis Berline Variety kurstaki, D제품 : B. thuringiensis var. kurstaki, E제품 : B. thuringiensis subsp aizawai)에 대한 특성을 조사하였다. 제품의 생물활성에 영향을 미칠 수 있는 활성포자수의 조사 결과, 각 각 제품에 표시되어 있는 숫자 보다 A B제품은 약 100배, D E제품은 약 10배로 감소되어 나타났고, C제품은 유사하게 나타나는 경향을 보였다. 5개 제품의 pH를 측정한 결과 A, B제품은 각각 pH 3.67, pH 3.73으로 나타났고, 나머지 3개 제품은 약 pH 5로 나타났다. 위상차현미경으로 관찰된 독소단백질의 형태에서는 C제품이 B. thuringiensis 고유의 뚜렷한 이중피라미드모양의 독소단백질 모습을 갖고 있었다. 또한 전자현미경으로 관찰된 A제품에서는 이중피라미드모양이 심하게 마모되어있는 것을 확인하였다. 한편, B. thuringiensis 제품을 작물에 사용하였을 때 균일하게 도포되는지 여부를 확인하기 위하여 제형의 균일도를 조사하였다. 그 결과 C D E 제품은 NA배지에 균일하게 균이 배양되었고, A B 제품은 NA배지 상에 균일성을 보이지 않았다. 제품상의 특성에서 가장 큰 차이를 보인 'A'와 'C'제품으로 배추좀나방 유충을 이용하여 생물검정을 한 결과, 두 제품 모두 추천 농도에서 48시간 이후 100% 사충율을 보였으나 'A'제품의 경우에는 희석 배수에 따라 사망률이 일정하지 않게 나타났다.

Keywords

References

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