Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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Effects of Mulberry Leaf Extract Feeding on Lipid Status of Rats Fed High Cholesterol Diets

뽕잎추출물이 고콜레스테롤 식이 흰쥐의 지질대사에 미치는 영향

  • Park, Surk-Hoon (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Jang, Mi-Jin (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Hong, Jung-Hee (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Rhee, Soon-Jae (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Choi, Kyung-Ho (Dept. of Food Science and Nutrition, Catholic University of Daegu) ;
  • Park, Mo-Ra (Dept. of Food Science and Nutrition, Sanju National University)
  • 박석훈 (대구가톨릭대학교 식품영양학과) ;
  • 장미진 (대구가톨릭대학교 식품영양학과) ;
  • 홍정희 (대구가톨릭대학교 식품영양학과) ;
  • 이순재 (대구가톨릭대학교 식품영양학과) ;
  • 최경호 (대구가톨릭대학교 식품영양학과) ;
  • 박모라 (국립상주대학교 식품영양학과)
  • Published : 2007.01.31
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Abstract

This study was to investigate the effect of mulberry leaf water extract on lipid metabolism of rats fed high cholesterol diets. Sprague-Dawley male rats were randomly assigned to two normal groups; mulberry extract-free (N), 0.16% mulberry leaf extract (NM) groups and high cholesterol groups with four different levels of mulberry leaf extract; 0% (HC), 0.08% (HCL), 0.16% (HCM), and 0.32% mulberry leaf extract (HCH) groups. Serum levels of triglyceride, cholesterol, LDL-cholesterol and AI index in mulberry leaf extract supplemented groups were significantly lower than the HC group (p<0.05). The level of HDL-cholesterol in the HC group was significantly (p<0.05) reduced, compared with N group, but it was increased by mulberry leaf extract supplementation. Mulberry leaf extract had no effect on the UDP-glucuronyl transferase activity. Also, there was no significant difference in the level of liver cholesterol between the HC and mulberry leaf extract supplemented groups, while there was significant difference in the levels of liver total lipid and triglyceride. The HCM and HCH groups had more significant reduction in the activity of lipoprotein lipase in epididymal fat tissue than the HC group. The levels of total lipid, triglyceride and total cholesterol in epididymal fat tissue of HCL, HCM and HCH groups were decreased compared to HC group. The levels of total lipid, triglyceride and total cholesterol in feces from HCL, HCM and HCH groups were higher than those of HC group.

본 연구는 뽕잎추출물의 지질대사 개선효과를 검정하고자 하였다. 실험 동물은 체중 100$\pm$10 g 내외의 Sprague-Dawley 종 수컷을 구입하여 난괴법 (randomized complet block design)에 의해 정상식이군(N group), 정상식이에 0.16% 뽕잎추출물 공급군(NM group), 고콜레스테롤식이 공급군(HC group)과 고콜레스테롤식이에 뽕잎추출물 공급군으로 나눈 후 다시 뽕잎추출물의 급여 수준에 따라 뽕잎추출물을 0.08% 공급한 군(HCL group), 뽕잎추출물을 0.16% 공급한 군(HCM group), 뽕잎추출물을 0.32% 공급한 군(HCH group) 등 각 군 모두 10마리씩 하였다. 식이와 식수는 자유 공급시켰으며 4주간 사육한 후 희생시켰다. 체중증가량, 식이섭취량, 식수섭취량 및 식이효율은 실험군간의 유의적인 차이는 없었다. 혈중 중성지방, 총콜레스테롤, LDL-콜레스테롤, A.I 등은 고콜레스테롤군에 비해 뽕잎추출물 공급군이 유의적으로 감소되었으며 HDL-콜레스테롤은 고콜레스테롤군에 비해 뽕잎추출물 공급군이 유의적으로 증가되었다. UDP-glucuronyl transferase(UDP-GTase) 활성은 N군에 비해 HC군에서 유의적으로 증가되었으나 고콜레스테롤 식이군간의 유의적인 차이는 없었다. 간조직중의 총지방 및 콜레스테롤 함량은 고콜레스테롤군에 비해 뽕잎추출물 공급군에서 유의적으로 감소되었다. 콜레스테롤 함량은 고콜레스테롤 식이군 간의 유의적인 차이가 없었다. Lipoprotein lipase활성은 HC군에 비하여 HCM 및 HCH군에서 유의적으로 감소되었다. 부고환 지방 중의 총지질, 중성지방, 콜레스테롤 함량은 HC군에 비해 뽕잎추출물 공급군에서 유의적으로 감소되었다. 분변 중으로 배설되는 총지질, 중성지방, 콜레스테롤 함량은 HC군에 비해 뽕잎추출물 공급군에서 증가되었다. 이상의 결과에서 뽕잎추출물의 고콜레스테롤 식이 흰쥐에서 혈액, 간, 부고환 조직 및 분변중의 지질대사 개선효과가 규명되었다.

Keywords

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