DOI QR코드

DOI QR Code

Vibrio tapetis의 검출을 위한 PCR specific primer의 제작

PCR Specific Primer for the Detection of Vibrio tapetis

  • 김영진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 이선이 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 조효진 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 유선녕 (부산대학교 의과대학 미생물학 및 면역학 교실) ;
  • 김철민 (부산대학교 의과대학 생화학 교실) ;
  • 최용락 (동아대학교 생명자원과학대학 응용생명 공학부) ;
  • 박병근 ((재)바이오21센터) ;
  • 안순철 (부산대학교 의과대학 미생물학 및 면역학 교실)
  • Kim, Yeong-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Lee, Sun-Yi (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Cho, Hyo-Jin (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • You, Sun-Nyung (Department of Microbiology and Immunology, College of Medicine, Pusan National University) ;
  • Kim, Cheol-Min (Department of Biochemistry, College of Medicine, Pusan National University) ;
  • Choi, Yong-Lark (Division of Biotechnology, Faculty of Natural Resources and Life Science, Dong-a University) ;
  • Park, Byoung-Keun (Bio21 Center) ;
  • Ahn, Soon-Cheol (Department of Microbiology and Immunology, College of Medicine, Pusan National University)
  • 발행 : 2007.01.29

초록

Brown Ring Disease (BRD) is a bacterial disease caused by Vibrio tapetis which affects cultured clam Ruditapes philippinarum and causes heavy economic losses on Atlantic coasts of france, Spain and England. In this study, to evaluate the effective detection of the pathogen, specific primer set based on 16S ribosomal RNA (rRNA) sequences designed for rapid detection of V. tapetis. Polymerase chain reaction (PCR) with this primer set produced the specific band for each V. tapetis. The length of PCR product using designed primer set of Vbts-F and Vbts-R was about 400 bp. Therefore, these primers will be provided with a basic tool for rapid detection of V. tapetis in the various cases such as examination of imported aquatic products, diagnosis of aquatic organisms, and etc.

키워드

참고문헌

  1. Alasina, M. and A. R. Blanch. 1994. Improvement and update of a set of keys for biochemical identification of Vibrio species. J. Appl. Bacteriol., 77, 719-721 https://doi.org/10.1111/j.1365-2672.1994.tb02824.x
  2. Allam, B., C. Paillard, and S. E. Ford. 2002. Pathogenicity of Vibrio tapetis, the etiologic agent brown ring disease in clams. Dis. Aquat. Org. 48, 221-231 https://doi.org/10.3354/dao048221
  3. Castro, D., J. A. Santamaria, A. Luque, E. Marinez-Manzanares, and J. J. Borrego. 1996. Antigenic characterization of the etiological agent of the brown ring disease affecting manila clams. Syst. Appl. Microbiol., 19, 231-239 https://doi.org/10.1016/S0723-2020(96)80049-X
  4. Elliot, E. L., C. A. Kaysner and M. L. Tamplin. 1992. Vibrio parahaemolyticus, V. cholerae, V. vulnificus and other Vibrio spp. In Food and Drug Administration. Bacteriological Analytical Manual, 7th ed. AOAC International, Arlington. pp. 111-140
  5. Euzeby, J. P. 1997. List of bacterial names with standing in nomenclature: a folder available on the Internet (URL: http://www.bacterio.cict.fr/). Int. J. Syst. Bacteriol. 47, 590-592 https://doi.org/10.1099/00207713-47-2-590
  6. Gurtler, V. and V. A. Stanisich. 1996. New approach to typing and identification of bacteria using the 16S-23S rDNA spacer region. Microbiology. 142, 3-6 https://doi.org/10.1099/13500872-142-1-3
  7. Jensen S., Ole B. Samuelsen, K. Andersen, L. Torkildsen, C. Lambert, G. Choquet, C. Paillard, and O. Bergh. 2003. Characterization of strains of Vibrio splendidus and V. tapetis isolated from corkwing wrasse Symphodus melops suffering vibriosis. Dis. Aquat. Org. 53, 25-31 https://doi.org/10.3354/dao053025
  8. Kim, J. H. and S. K Chun, 1990. A Vibriosis occurring in cultured kuruma prawn Penaeus japonicus. J. Fish Pathol., 3 (1), 1-9
  9. Krawiec, S. and M. Riley. 1990. Organization of the bacterial chromosome. Microbiol. Rev. 54, 502-539
  10. Miranda, C. D., C. Kehrenberg, C. Ulep, S. Schwarz, and M. C. Roberts. 2003. Diversity of tetracycline resistance genes in bacteria from Chilean Salmon farms. Antimicrobial Agents and Chemotherapy. 47(3), 883-888 https://doi.org/10.1128/AAC.47.3.883-888.2003
  11. Parish, T. and N. G. Stoker. 2002. The common aromatic amino acid biosynthesis pathway is essential in Mycobacterium tuberculosis. Microbiology. 148, 3069-3077 https://doi.org/10.1099/00221287-148-10-3069
  12. Park, S. W. and K. H. Lee. 2005. Pathogenicity and PCR detection of Vibrio tapetis in Manila clams, Ruditapes philippinarum. J. Fish Pathol., 18(1), 39-48
  13. Park, S. W., Y. G. Kim, and D. L. Choi. 1996. Vibrio ordalii, the causative agent of massive mortality in cultured rockfish(Sebastes schlegeli) larvae. J. Fish Pathol., 9(2), 137-145
  14. Rheiheimer, G. 1992. Aquatic Microbiology. Wiley, London, U.K., pp. 364
  15. Yang, H. C., S. S. Hong, K. H. Kim, S. H. Choi, and H. J. Chung. 1999. Distribution of Vibrio vulnificus in Chonnam coastal area. Kor. J. Appl. Microbiol. Biotechnol. 27(1), 70-74