Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
권호 표지
DOI QR코드

DOI QR Code

The Effect of Dietary Fat on Insulin Secretion and Pancreatic β-Cell Mass in 90% Pancreatectomized Diabetic Rats

식이 지방이 췌장 90%를 제거한 당뇨 흰쥐의 인슐린 분비능과 췌장 베타세포의 양에 미치는 영향

  • Park, Sun-Min (Dept. of Food & Nutrition, College of Natural Science, Hoseo University) ;
  • Park, Chun-Hee (Dept. of Food & Nutrition, College of Natural Science, Hoseo University) ;
  • Hong, Sang-Mee (Dept. of Food & Nutrition, College of Natural Science, Hoseo University)
  • 박선민 (호서대학교 자연과학대학 식품영양학과) ;
  • 박춘희 (호서대학교 자연과학대학 식품영양학과) ;
  • 홍상미 (호서대학교 자연과학대학 식품영양학과)
  • Published : 2007.02.28
Download PDF
⟨ Previous Next ⟩

Abstract

The prevalence of diabetes has increased to 8% of population. Unlike type 2 diabetes in the western countries, Korean diabetic patients are nonobese and have low serum insulin levels. As the increased prevalence of diabetes and the peculiar characteristics may be related to dietary fat contents, we determined their effects on insulin resistance, insulin secretion and pancreatic $\beta-cell$ mass in 90% pancreatectomized (Px) diabetic rats in the present study. The rats were provided with low fat diet (LF, 10 energy% fat), moderate fat diet (MF, 25 energy% fat) and high fat diet (HF, 40 energy% fat) for 6 months. HF increased body weight and epidydimal fat pads parallel with increased food intake compared to LF and MF. Fasting serum glucose and insulin levels and homeostasis model assessment of insulin resistance were higher in HF, compared to LF and MF, indicating that HF increased insulin resistance. Rats fed LF and MF diets reduced insulin resistance, but only rats fed MF improved pancreatic $\beta-cell$ mass and insulin secretion capacity, measured by hyperglycemic clamp and in situ pancreatic perfusion. LF had low insulin secretion capacity and pancreatic $\beta-cell$ mass, indicating the increased possibility of diabetic prevalence and progression. MF increased $\beta-cell$ mass by stimulating $\beta-cell$ proliferation and neogenesis and reducing $\beta-cell$ apoptosis. In conclusion, MF is effective for the prevention of prevalence and progression of diabetes.

Keywords

References

  1. Huh KB. 1992. Insulin resistance and chronic degenerative diseases. Kr J Diabetes 16: 93-98
  2. Kim EJ, Kim YK. 1970. Epidemiology of diabetes in Korean diabetics. Kr J Internal Med 13: 25-29
  3. Korean Medical Insurance Association. 1993. 1992 Annuals for medical insurance statistics. Ministry of Health and Welfare, Seoul
  4. Min HK. 1996. Non-insulin-dependent diabetes mellitus (NIDDM) in Korea. Diabet Med 13: S13-S15
  5. Sung YA, HongYS. 2000. Mechanism of the insulin secretory defect by chronically elevated glucose levels in pancreatic islets: depletion of insulin content due to hyperstimulation by glucose. Kr J Diabetes 24: 1-9
  6. Leahy JL. 1990. Natural history of beta cell dysfunction in NIDDM. Diabetes Care 13: 992-1010 https://doi.org/10.2337/diacare.13.9.992
  7. Rossetti L, Giaccari A, DeFronzo RA. 1990. Glucose toxicity. Diabetes Care 13: 610-630 https://doi.org/10.2337/diacare.13.6.610
  8. Withers DJ, Burks DJ, Towery HH, Altamuro SL, Flint CL, White MF. 1999. Irs-2 coordinates Igf-1 receptor-mediated beta-cell development and peripheral insulin signalling. Nat Genet 23: 32-40 https://doi.org/10.1038/12631
  9. White MF. 2003. Insulin signaling in health and disease. Science 302: 1710-1711 https://doi.org/10.1126/science.1092952
  10. Hennige AM, Burks DJ, Ozcan U, Kulkarni RN, Ye J, Park S, Schubert M, Fisher TL, Dow MA, Leshan R, Zakaria M, Mossa-Basha M, White MF. 2003. Upregulation of insulin receptor substrate-2 in pancreatic $\beta$-cells prevents diabetes. J Clin Invest 112: 1521-1532 https://doi.org/10.1172/JCI200318581
  11. Ye JM, Dzamko N, Hoy AJ, Iglesias MA, Kemp B, Kraegen E. 2006. Rosiglitazone treatment enhances acute AMP-activated protein kinase-mediated muscle and adipose tissue glucose uptake in high-fat-fed rats. Diabetes 55: 2797- 2804 https://doi.org/10.2337/db05-1315
  12. Tokuyama Y, Sturis J, DePaoli AM, Takeda J, Stoffel M, Tang J, Sun X, Polonsky KS, Bell GI. 1995. Evolution of beta-cell dysfunction in the male Zucker diabetic fatty rat. Diabetes 44: 1447-1457 https://doi.org/10.2337/diabetes.44.12.1447
  13. Corsetti JP, Sparks JD, Peterson RG, Smith RL, Sparks CE. 2000. Effect of dietary fat on the development of non-insulin dependent diabetes mellitus in obese Zucker diabetic fatty male and female rats. Atherosclerosis 148: 231-241 https://doi.org/10.1016/S0021-9150(99)00265-8
  14. Choi SB, Park S. 1999. The effect of w-6 polyunsaturated fat and vitamin-E on insulin resistance in 90% pancreatectomized rats. Kr J Internal Med 56: 490-499
  15. Park S, Park CH, Jang JS. 2006. Antecedent intake of traditional Asian-style diets exacerbates pancreatic $\beta$-cell function, growth and survival after Western-style diet feeding in weaning male rats. J Nutr Biochem 17: 307-318 https://doi.org/10.1016/j.jnutbio.2005.07.002
  16. Hosokawa YA, Hosokawa H, Chen C, Leahy JL. 1996. Mechanism of impaired glucose-potentiated insulin-secretion in diabetic 90 percent pancreatectomy rats-study using glucagon like peptide-1(7-37). J Clin Invest 97: 180-186 https://doi.org/10.1172/JCI118387
  17. Park JS, Kim CS, Nam JY, Kim DM, Jo MH, Park J, Ahn CW, Cha BS, Lim SK, Kim KR, Lee HC, Huh KB. 2005. Characteristics of type 2 diabetes in terms of insulin resistance in Korea. Yonsei Med J 46: 484-490 https://doi.org/10.3349/ymj.2005.46.4.484
  18. Chae BN, Lee SK, Hong EG, Chung YS, Lee KW, Kim HM. 1998. The role of insulin secretion and insulin resistance in the development of Korean type 2 diabetes mellitus. Kr J Diabetes 22: 491-503
  19. Report of the American Institute of Nutrition. 1977. Ad hoc committee on standards for nutritional studies. J Nutr 107: 1340-1348 https://doi.org/10.1093/jn/107.7.1340
  20. Thule PM, Campbell AG, Kleinhenz DJ, Olson DE, Boutwell JJ, Sutliff RL, Hart CM. 2006. Hepatic insulin gene therapy prevents deterioration of vascular function and improves adipocytokine profile in STZ-diabetic rats. Am J Physiol Endocrinol Metab 290: E114-E122 https://doi.org/10.1152/ajpendo.00134.2005
  21. Rossetti L, Shulman GI, Zawalich W, DeFronzo RA. 1987. Effect of chronic hyperglycemia on in vivo insulin secretion in partially pancreatectomized rats. J Clin Invest 80: 1037-1044 https://doi.org/10.1172/JCI113157
  22. Thams P, Capito K. 1999. L-arginine stimulation of glucose-induced insulin secretion through membrane depolarization and independent of nitric oxide. Euro J Endocrinol 140: 87-93 https://doi.org/10.1530/eje.0.1400087
  23. Choi SB, Jang JS, Park S. 2005. Estrogen and exercise may enhance beta-cell function and mass via insulin receptor substrate 2 induction in ovariectomized diabetic rats. Endocrinology 146: 4786-4794 https://doi.org/10.1210/en.2004-1653
  24. Rooman I, Lardon J, Bouwen L. 2002. Gastrin stimulates $\beta$-cell neogenesis and increases islet mass from transdifferentiated but not from normal exocrine pancreas tissue. Diabetes 51: 686-690 https://doi.org/10.2337/diabetes.51.3.686
  25. Lee JH, Son HS, Kim JY, Park SY, Lee SK. 1994. Effect of high fat diet on the glycogen synthase activity of the different skeletal muscles in rats. Kr J Diabetes 18: 21-25
  26. Unger RH. 1995. Lipotoxicity in the pathogenesis of obesity-dependent NIDDM. Diabetes 44: 863-870 https://doi.org/10.2337/diabetes.44.8.863
  27. Weir GC, Bonner-Weir S. 2004. Five stages of evolving beta-cell dysfunction during progression to diabetes. Diabetes 53: S16-S21 https://doi.org/10.2337/diabetes.53.suppl_3.S16
  28. Laybutt DR, Glandt M, Xu G, Ahn YB, Trivedi N, Bonner-Weir S, Weir GC. 2003. Critical reduction in beta-cell mass results in two distinct outcomes over time. Adaptation with impaired glucose tolerance or decompensated diabetes. J Biol Chem 278: 2997-3005 https://doi.org/10.1074/jbc.M210581200
  29. Sharma A, Zangen DH, Reitz P, Taneja M, Lissauer ME, Miller CP, Weir GC, Habener JF, Bonner-Weir S. 1999. The homeodomain protein IDX-1 increases after an early burst of proliferation during pancreatic regeneration. Diabetes 48: 507-513 https://doi.org/10.2337/diabetes.48.3.507
  30. Ko SH, Suh SH, Kim BJ, Ahn YB, Song KH, Yoo SJ, Son HS, Cha BY, Lee KW, Son HY, Kang SK, Bonner-Weir S, Weir GC, Yoon KH, Park CG. 2004. Expression of the intermediate filament vimentin in proliferating duct cells as a marker of pancreatic precursor cells. Pancreas 28: 121-128 https://doi.org/10.1097/00006676-200403000-00002
  31. Tamemoto H, Kadowaki T, Tobe K, Yagi T, Sakura H, Hayakawa T, Terauchi Y, Ueki K, Kaburagi Y, Satoh S. 1994. Insulin resistance and growth retardation in mice lacking insulin receptor substrate-1. Nature 372: 128-129 https://doi.org/10.1038/372182a0

Cited by

  1. Exercise improves glucose homeostasis that has been impaired by a high-fat diet by potentiating pancreatic β-cell function and mass through IRS2 in diabetic rats vol.103, pp.5, 2007, https://doi.org/10.1152/japplphysiol.00434.2007