Protective Effect of Water Extract of Fraxinus Rhynchophylla Leaves on Acetaminophen-induced Nephrotoxicity in Mice and Its Phenolic Compounds

  • Jeon, Jeong-Ryae (Department of Food and Nutrition, Institute of Medical Science, Yeungnam University, College of Medicine) ;
  • Choi, Joon-Hyuk (Department of Pathology, Yeungnam University, College of Medicine)
  • 발행 : 2007.12.31

초록

The protective effect of the water extract of Fraxinus rhynchophylla leaves (FLE) was determined using an animal model of acetaminophen (AAP)-induced nephrotoxicity. The BALB/c male mice used in this study were divided into 3 groups; the normal, AAP-administered, and FLE-pretreated AAP groups. A single dose of AAP induced necrosis of renal tubules and congestion along with edema to a remarkable degree as observed by hematoxylin and eosin stain, and also increased the numbers of terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate nick end labeling (TUNEL)-positive renal tubular epithelial cells. Blood urea nitrogen and plasma creatinine levels were determined to be significantly higher in the AAP group than in the normal group. However, FLE pretreatment resulted in an attenuation of renal tubule necrosis. Regeneration and dilatation of renal tubules were noted, and the numbers of TUNEL-positive cells were reduced in the FLE-pretreated groups. In an effort to detect the bioactive compounds exerting protective effects in FLE, the analysis of phenolic compounds via gas chromatography/mass spectrometry (GC/MS) were performed, and identified esculetin and esculin. The present study indicates that these compounds may exert a protective effect against AAP-induced nephrotoxicity.

키워드

참고문헌

  1. Ismail M, Manickam E, Danial AM, Rahmat A, Yahaya A. Chemical composition and antioxidant activity of Strobilanthes crispus leaf extract. J. Nutr. Biochem. 11: 536-542 (2000) https://doi.org/10.1016/S0955-2863(00)00108-X
  2. Jeon JR, Kim JY. Effect of pine needle extract on differentiation of 3T3 L1 preadipocytes and obesity in high fat diet-fed rats. Biol. Pharm. Bull. 29: 2111-2115 (2006) https://doi.org/10.1248/bpb.29.2111
  3. Jeon JR, Jin TY, Kim J, Park JR. Chemical composition of Smilax china leaves and quality characteristics of rice cakes. Food Sci. Biotechnol. 15: 660-611 (2006)
  4. Marini-Bettolo GB. Present aspects of the use of plants in traditional medicine. J. Ethnopharmacol. 2: 5-7 (1980) https://doi.org/10.1016/0378-8741(80)90021-5
  5. Xiao PG, Li DP, Yang SL. Cortex fraxini. Vol. 3, p. 633. In: Modern Chinese Materia Media. Xiao PG (ed). Chemistry Industry Publisher, Beijing, China (2002)
  6. Kwon YS, Kim CM. A study on the chemical constituents from leaves of Fraxinus rhynchophylla. Korean J. Pharmacol. 27: 347- 349 (1996)
  7. Tsai JC, Tsai S, Chang WC. Effect of ethanol extracts of three Chinese medicinal plants with anti-diarrheal properties on ion transport of the rat intestinal epithelia. J. Pharmacol. Sci. 94: 60-66 (2004) https://doi.org/10.1254/jphs.94.60
  8. Jeon JR, Choi JH. Effect of ash tree leaf extract on acetaminopheninduced hepatotoxicity in mice. Food Sci. Biotechnol. 15: 752-755 (2006)
  9. Jeon JR. Water extract of ash tree (Fraxinus rhynchophylla) leaves protects against paracetamol-induced oxidative damages in mice. Food Sci. Biotechnol. 15: 612-617 (2006)
  10. Sener G, Sehirli O, Cetinel S, Ye en BG, Gedik N, Ayano lu- Dügler G. Protective effects of MESNA (2-mercaptoethane sulphonate) against acetaminophen-induced hepatorenal oxidative damage in mice. J. Appl. Toxicol. 25: 20-29 (2005) https://doi.org/10.1002/jat.1012
  11. McLean A. Models of liver disease. pp.2-12. In: New Trends in the Therapy of Liver Diseases. Karger BA (ed). S Karger AG, New York, NY, USA (1975)
  12. Mitchell JR, Jollow DJ, Potter WZ, Gillette JR, Brodie BB. Acetaminophen-induced hepatic necrosis. IV. Protective role of glutathione. J. Pharmacol. Exp. Ther. 187: 211-217 (1973)
  13. Li C, Chen A, Chen X, Ma X, Chen X, Hu Z. Study of antibacterial function of cortex fraxini. Biomed. Chromatogr. 19: 696-702 (2005) https://doi.org/10.1002/bmc.497
  14. Hu SG, Li L, He XW. Solid-phase extraction of esculetin from the ash bark of Chinese traditional medicine by using molecularly imprinted polymers. J. Chromatogr. A 1062: 31-37 (2005) https://doi.org/10.1016/j.chroma.2004.11.036
  15. Zhang HY, Li QF, Shi ZH, Hu ZD, Wang R. Determination of fraxelin A and fraxetin B. The bark of Fraxinus rhynchophylla by HPLC. Talanta 52: 607-621 (2000) https://doi.org/10.1016/S0039-9140(00)00343-X
  16. Price ML, Butler LG. Rapid visual estimation and spectrophotometric determination of tannin content of sorghum grain. J. Agr. Food Chem. 25: 1268-1273 (1977) https://doi.org/10.1021/jf60214a034
  17. Kim JB, Park JR. The simultaneous determination of phenolic compounds by GC and GC/MS. J. Food Sci. Nutr. 3: 111-118 (1998)
  18. Wang CJ, Hsieh YJ, Chu CY, Lin YL, Tseng TH. Inhibition of cell cycle progression in human leukemia HL-60 cells by esculin. Cancer Lett. 183: 163-168 (2002) https://doi.org/10.1016/S0304-3835(02)00031-9
  19. Chang WS, Change YH, Lu FJ, Chiang HC. Inhibitory effects of phenolics on xanthine oxidase. Anticancer Res. 14: 501-506 (1994)
  20. Lin WL, Wang CJ, Tsai YY, Liu CL, Hwang JM, Tseng TH. Inhibitory effect of esculetin on oxidative damage induced by tbutyl hydroperoxide in rat liver. Arch. Toxicol. 74: 467-472 (2000) https://doi.org/10.1007/s002040000148
  21. Neichi T, Koshihara Y, Murota S. Inhibitory effect of esculetin on 5- lipoxygenase and leukotriene biosynthesis. Biochim. Biophys. Acta 753: 130-132 (1983) https://doi.org/10.1016/0005-2760(83)90106-6