Protective Effect of Acanthopanax senticosus on Oxidative Stress Induced PC12 Cell Death

  • Choi, Soo-Jung (Graduate School of Biotechnology, Korea University) ;
  • Yoon, Kyung-Young (Department of Food and Nutrition, Yeungnam University) ;
  • Choi, Sung-Gil (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Kim, Dae-Ok (Department of Food Science and Technology, Institute of Life Science and Resources, Kyung Hee University) ;
  • Oh, Se-Jong (Department of Animal Science, Institute of Agriculture Science and Technology, Chonnam National University) ;
  • Jun, Woo-Jin (Department of Food and Nutrition, Chonnam National University) ;
  • Shin, Dong-Hoon (Graduate School of Biotechnology, Korea University) ;
  • Cho, Sung-Hwan (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University) ;
  • Heo, Ho-Jin (Division of Applied Life Science, Institute of Agriculture and Life Sciences, Gyeongsang National University)
  • Published : 2007.12.31

Abstract

Epidemiologic studies have shown important relationships between oxidative stress and Alzheimer's disease (AD) brain. In this study, free radical scavenging activity and neuronal cell protection effect of aqueous methanol extracts of Acanthopanax senticosus (A. senticosus) were examined. $H_2O_2$-induced oxidative stress was measured using 2',7'-dichlorofluorescein diacetate (DCF-DA) assay. Pretreatment with the phenolics of A. senticosus prevented oxidative injury against $H_2O_2$ toxicity. Since oxidative stress is known to increase neuronal cell membrane breakdown, leading to cell death, lactic dehydrogenase release, and trypan blue exclusion assays were utilized. We found that phenolics of A. senticosus have neuronal cell protection effects. It suggests that the phenolics of A. senticosus inhibited $H_2O_2$-induced oxidative stress and A. senticosus may be beneficial against the oxidative stress-induced risk in AD.

Keywords

References

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