Antimicrobial Activity of an Edible Wild Plant, Apiifolia Virgin's Bower (Clematis apiifolia DC)

  • Kyung, Kyu-Hang (Department of Food Science, Sejong University) ;
  • Woo, Yong-Ho (Department of Food Science, Sejong University) ;
  • Kim, Dong-Sub (Department of Hotel Culinary Arts, Shin Heung Junior College) ;
  • Park, Hun-Jin (Department of Western Cuisine and Culinary Arts, Youngsan University) ;
  • Kim, Youn-Soon (Department of Home Economy Education, Chosun University)
  • Published : 2007.12.31


An edible wild perennial plant with extremely potent antimicrobial activity was found and identified as apiifolia Virgin's Bower (Clematis apiifolia DC) which is easily found around wet wildernesses. Fresh fruit extract of C. apiifolia exhibited minimum inhibitory concentrations (MIC) in the vicinity of 0.1% against various yeasts and of less than or equal to 0.4% for non-lactic acid bacteria. MICs against lactic acid bacteria were about 2.0%. The antimicrobial activity of C. apiifolia fruit was even more potent than that of garlic which has been known for its potent antimicrobial activity. The principal antimicrobial compound of fruit extract of C. apiifolia was isolated and identified by high performance liquid chromatography and gas chromatography as protoanemonin (a gamma lactone of 4-hydroxy-2,4-pentadienoic acid). The antimicrobial activity of C. apiifolia was stable at high temperatures, and the activity was maintained after heating at $121^{\circ}C$ for 10 min. The antimicrobial compound of C. apiifolia was supposed to inhibit microorganisms by reacting with sulfhydryl groups of cellular proteins.


  1. Hostettmann K, Potterat O, Wolfender J. The potential of higher plants as a source of new drugs. Chimia 52: 10-17 (1998)
  2. Hostettmann K, Marston A. Search for new antifungal compounds from higher plants. Pure Appl. Chem. 66: 2231-2234 (1994)
  3. Cowan M. Plant products as antimicrobial agents. Clin. Microbiol. Rev. 12: 564-582 (1999)
  4. Larson AE, Yu RR, Lee OA, Price S, Haas GJ, Johnson EA. Antimicrobial activity of hop extracts against Listeria monocytogenes in media and in food. Int. J. Food Microbiol. 33: 195-207 (1996)
  5. Vattem DA, Lin YT, Ghaedian R, Shetty K. Cranberry synergies for dietary management of Helicobacter pylori infections. Process Biochem. 40: 1583-1592 (2005)
  6. Lin YT, Labbe RG, Shetty K. Inhibition of Listeria monocytogenes in fish and meat systems using oregano and cranberry synergies. Appl. Environ. Microb. 70: 5672-5678 (2004)
  7. Yeo EJ, Kim KT, Han YS, Nah SY, Paik HD. Antimicrobial, antiinflammatory, and anti-oxidative activities of Scilla scilloides (Lindle.) druce root extract. Food Sci. Biotechnol. 15: 639-642 (2006)
  8. Lin YT, Kwon YI, Labbe RG, Shetty K. Inhibition of Helocobacter pylori and associated urease by oregano and cranberry phytochemical synergies. Appl. Environ. Microb. 71: 8558-8564 (2004)
  9. Le-Dinh H, Kyung KH. Inhibition of yeast film formation in fermented vegetables by materials derived from garlic using cucumber pickle fermentation as a model system. Food Sci. Biotechnol. 15: 469-473 (2006)
  10. Choi MK, Chae KY, Lee JY, Kyung KH. Antimicrobial activity of chemical substances derived from S-alk(en)yl-L-cysteine sulfoxide (alliin) in garlic, Alliium sativum L. Food Sci. Biotechnol. 16: 1-7 (2007)
  11. Mares D. Antimicrobial activity of protoanemonin, a lactone from ranunculaceous plant. Mycopathologia 98: 133-140 (1987)
  12. Baer H, Holden M, Seegal BC. The nature of the antibacterial agent from Anemone pulsatilla. J. Biol. Chem. 162: 65-68 (1945)
  13. O'Gara EA, Hill DJ, Maslin DJ. Activity of garlic oil, garlic powder, and their diallyl constituents against Helicobacter pylori. Appl. Environ. Microb. 66: 2269-2273 (2000)
  14. Johnson MG, Vaughn RH. Death of Salmonella typhimurium and Eschericerichia coli in the presence of freshly reconstituted dehydrated garlic and onion. Appl. Microbiol. 17: 903-905 (1969)
  15. Kim JW, Kyung KH. Antiyeast activity of heated garlic in the absence of alliinase enzyme action. J. Food Sci. 68: 1766-1770 (2003)
  16. Kyung KH, Fleming HP. S-Methyl-L-cysteine sulfoxide as the precursor of methyl methanethiosulfinate, the principal antibacterial compound in cabbage. J. Food Sci. 59: 350-355 (1994)
  17. Korea forest service. Available from: plantGuide/results/view_detail.jsp?name_id=6670&returnUrl. Accessed Jan. 3, 2007
  18. Lee YN. Flora of Korea. Kyo-Hak Publishing Co., Seoul, Korea. p.162 (2002)
  19. Campbell WE, Cragg GML, Powrie AH. Anemonin, protoanemonin, and ranunculin from knowltonia capensis. Phytochemistry 18: 323- 324 (1979)
  20. Cavallito CJ, Heskell TH. The mechanism of action of antibiotics. The reaction of unsaturated lactones with cysteine and related compounds. J. Am. Chem. Soc. 67: 1991-1994 (1945)
  21. Cavallito CJ, Bailey JH. Allicin, the antibacterial principle of alliium sativum. I. Isolation, physical properties, and antimicrobial action. J. Am. Chem. Soc. 66: 1950-1951 (1944)
  22. Seegal BC, Holden M. The antibiotic activity of extracts of Ranunclaceae. Science 101: 313-414 (1945)
  23. Herz W, Pates AL, Madsen GC. The antimicrobial principle of Clematis dioscoreifolia. Science 114: 206 (1951)
  24. Nadir M, Abdual-Baqi D, Al-Sarraj S, Hussein W. The effect of different methods of extraction on the antimicrobial activity of medicinal plants. Fitoterapia 57: 359-363 (1986)