Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Mouse Strain-Dependent Osteoclastogenesis in Response to Lipopolysaccharide

  • Choi, Ho-Gil (Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Kim, Jin-Moon (Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Kim, Bong-Ju (Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Yoo, Yun-Jung (Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry) ;
  • Cha, Jeong-Heon (Department of Oral Biology, BK21 project, Oral Science Research Center, Yonsei University College of Dentistry)
  • Published : 2007.12.31
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Abstract

Bacterial lipopolysaccharide (LPS) is a potent stimulator of bone resorption in periodontitis. Co-culture systems of mouse calvaria-derived osteoblasts and bone marrow-derived preosteoclasts were used as an in vitro osteoclast differentiation. This study revealed that co-cultures using ddY or ICR mouse strain responded differently to LPS while responded equally to $1{\alpha},25(OH)_2D_3$. Thus, the different response to LPS indicates dissimilarity of two mouse stains in their capacity for generating osteoclasts while the two mouse strains share the similarity in response to $1{\alpha},25(OH)_2D_3$. To identify which cells between osteoblasts and preosteoclasts in the co-culture are responsible for the dissimilarity, the reciprocal co-cultures were performed between ddY and ICR mouse strains. The treatment of $1,25(OH)_2D_3$ to ddY/ICR (osteoblasts from ddY/preosteoclasts from ICR) and ICR/ddY reciprocal co-cultures also showed the similarity. In case of LPS treatment, the results of ddY/ICR were similar to ddY/ddY and the results of the other reciprocal co-culture, ICR/ddY combination, were consistent with those of ICR/ICR. It suggests that the dissimilarity between the two mouse strains may resident in osteoblasts but not in preosteoclasts. Therefore, the osteoblast is responsible for mouse strain-dependent osteoclastogenesis in response to LPS. Although mouse models will continue to provide insights into molecular mechanisms of osteoclastogenesis, caution should be exercised when using different mouse strains, especially ddY and ICR strains as models for osteoclast differentiation.

Keywords

References

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