Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Microbial Conversion of Ginsenoside $Rb_1$ to Minor Ginsenoside $F_2$ and Gypenoside XVII by Intrasporangium sp. GS603 Isolated from Soil

  • Cheng, Le-Qin (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Na, Ju-Ryun (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Kim, Myung-Kyum (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Bang, Myun-Ho (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University) ;
  • Yang, Deok-Chun (Department of Oriental Medicinal Material and Processing, College of Life Science, Kyung Hee University)
  • Published : 2007.12.31
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Abstract

A new strain, GS603, having ${\beta}$-glucosidase activity was isolated from soil of a ginseng field, and its ability to convert major ginsenoside $Rb_1$ to minor ginsenoside or gypenoside was studied. Strain GS603 was identified as an Intrasporangium species by phylogenetic analysis and showed high ginsenoside-converting activity in LB and TSA broth but not in nutrient broth. The culture broth of the strain GS603 could convert ginsenoside $Rb_1$i into two metabolites, which were analyzed by TLC and HPLC and shown to be the minor ginsenoside $F_2$ and gypenoside XVII by NMR.

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References

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