Antibacterial Activity of Essential Oils on the Growth of Staphylococcus aureus and Measurement of their Binding Interaction Using Optical Biosensor

  • Chung, Kyong-Hwan (Center for Functional Nano Fine Chemicals, Chonnam National University) ;
  • Yang, Ki-Sook (Interdisciplinary Program of Perfume and Cosmetics Graduate School, Chonnam National University) ;
  • Kim, Jin (Department of Advanced Chemicals, Chonnam National University) ;
  • Kim, Jin-Chul (School of Biotechnology & Bioengineering, Kangwon National University) ;
  • Lee, Ki-Young (Center for Functional Nano Fine Chemicals, Chonnam National University)
  • Published : 2007.11.30


Antibacterial activity of essential oils (Tea tree, Chamomile, Eucalyptus) on Staphylococcus aureus growth was evaluated as well as the essential oil-loaded alginate beads. The binding interactions between the cell and the essential oils were measured using an optical biosensor. The antibacterial activity of the essential oils to the cell was evaluated with their binding interaction and affinity. The antibacterial activity appeared in the order of Tea Tree>Chamomile>Eucalyptus, in comparison of the inhibition effects of the cell growth to the essential oils. The association rate constant and affinity of the cell binding on Tea Tree essential oil were $5.0{\times}10^{-13}\;ml/(CFU{\cdot}s)$ and $5.0{\times}10^5\;ml/CFU$, respectively. The affinity of the cell binding on Tea Tree was about twice higher than those on the other essential oils. It might be possible that an effective antibacterial activity of Tea Tree essential oil was derived from its strong adhesive ability to the cell, more so than those of the other essential oils.


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