Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Changes in Aurantio-Obtusin and Glucoaurantio-Obtusin Content in Cassiae Semen via Treatment with a Crude Enzyme Extract from Aspergillus usamii

  • Hur, Jong-Moon (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Kwon, Soon-Ho (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • So, Jae-Hyun (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Jun, Mi-Ra (Division of Food Science, Dong-A University) ;
  • Kang, Young-Hwa (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Lee, Yu-Mi (Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University) ;
  • Lee, Kyung-Bok (College of Medicine, Konyang University) ;
  • Rhee, In-Koo (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University) ;
  • Lee, Moon-Soon (Chemical Safety & Accidental Prevention Division, Chemical Assessment Department, National Institute of Environmental Research) ;
  • Song, Kyung-Sik (School of Applied Biosciences, College of Agriculture & Life Sciences, Kyungpook National University)
  • Published : 2007.11.30
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Abstract

Cassiae Semen (seeds of Cassia tora) showed a remarkably different HPLC chromatogram after being treated with a crude enzyme extract from Aspergillus usamii. Increased and decreased compounds were identified as aurantio-obtusin and glucoaurantio-obtusin, respectively. The aurantio-obtusin content reached its maximum level ($133.58{\pm}0.39\;{\mu}g/mg$ extract) after being incubated for 50 min at $37^{\circ}C$, whereas the inactivated crude enzyme-treated control remained unchanged ($54.13{\pm}1.33\;{\mu}g/mg$). On the other hand, the glucoaurantio-obtusin content decreased by less than one-third ($51.09{\pm}1.63\;{\mu}g/mg$) ofthe untreated control ($143.19{\pm}2.12\;{\mu}g/mg$), suggesting that an increase in aurantio-obtusin content originated from the enzymatic cleavage of its glucoside glucoaurantio-obtusin.

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