한국환경농학회지 (Korean Journal of Environmental Agriculture)

  • 제26권4호
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  • Pages.319-324
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  • 2007
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  • 1225-3537(pISSN)
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  • 2233-4173(eISSN)
한국환경농학회 (The Korean Society of Environmental Agriculture)
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포장 조건에서 CMVP0-CP 형질전환 고추 도입유전자의 지속성 조사

Assessment of the Persistence of DNA in Decomposing Leaves of CMVP0-CP Transgenic Chili Pepper in the Field Conditions

  • 이범규 (한국생명공학연구원 바이오평가센터) ;
  • 김창기 (한국생명공학연구원 바이오평가센터) ;
  • 박지영 (한국생명공학연구원 바이오평가센터) ;
  • 박기웅 (한국생명공학연구원 바이오평가센터) ;
  • 이훈복 (한국생명공학연구원 바이오평가센터) ;
  • 한지학 ((주)농우바이오 생명공학연구소) ;
  • 김환묵 (한국생명공학연구원 바이오평가센터)
  • Lee, Bum-Kyu (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Chang-Gi (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Ji-Young (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Park, Kee-Woong (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Yi, Hoon-Bok (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology) ;
  • Harn, Chee-Hark (Biotechnology Institute, Nongwoo Bio Co. Ltd.) ;
  • Kim, Hwan-Mook (Bio-Evaluation Center, Korea Research Institute of Bioscience and Biotechnology)
  • 발행 : 2007.12.31
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초록

오이모자이크바이러스(cucumber mosaic virus, CMV)에 저항성을 가지도록 개발된 형질전환 CMVP0-CP 고추를 이용하여 포장 조건에서 CMVP0-CP 고추 도입유전자의 지속성을 조사하였다. CMVP0-CP 고추의 잎을 토양 표면에 올려놓거나 토양 내에 묻은 뒤 일정 시간 간격으로 PCR 및 실시간 PCR법을 이용하여 도입유전자를 정성, 정량 분석하였다. CMVP0-CP 고추에 도입된 유전자의 양은 10 cm 깊이의 토양 속 및 토양 표면에서 1개월 후 28.3-42.7%, 2개월 후 0.9-3.3%로 감소하였으며, 3-4개월 후에는 검출되지 않았다. 또한 이러한 유전자는 지중보다 지표에서 약8% 더 오래 지속되었다. CMVP0-CP 고추의 잎이 토양 내에서 분해되는 과정에서 유출된 DNA가 주변 토양으로 전이되었는지를 조사하기 위해 낙엽주머니에 부착된 토양으로부터 DNA를 추출하여 PCR분석을 수행하였다. PCR 분석 결과 CMVP0-CP 고추에 도입된 유전자가 검출되지 않았다.

This study was conducted to evaluate the persistence of DNA in the transgenic chili pepper resistant to cucumber mosaic virus (CMV) in the field condition. We analyzed the persistence of genes in the leaf samples obtained from two field conditions, below and above soil. Transgenic and non-transgenic leaf tissues were buried in the soil at a depth of 10 cm or placed on the soil surface. Qualitative and quantitative PCR analysis showed that the amount of transferred genes from the transgenic peppers below and above soil was dropped to 28.3-42.7% one month after buried and it was rapidly reduced to 0.9-3.3% after two months. The transgenes were not detected three to four month after buried. In addition, DNA of the leaves placed below soil decomposed about 8%more than those on soil surface. The gene transfer from decomposing leaves of the transgenic pepper to soil was investigated by PCR analysis with the soil attached to the samples. The PCR result indicated that the gene transfer from the transgenic pepper to soil was not occurred.

키워드

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