Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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The Effects of Genistein on the Proliferation and Type I pN Collagen Synthesis in Aged Normal Human Fibroblasts

제니스테인에 의한 노화된 피부세포 활성화와 콜라겐 생성 효과

  • Yang, Eun-Soon (Department of Bioengineering, graduate School at KonKuk University) ;
  • Hong, Ran-Hi (Department of Preventive Medicine, College of Medicine, ChungAng University) ;
  • Kang, Sang-Mo (Department of Bioengineering, graduate School at KonKuk University)
  • 양은순 (건국대학교 대학원 생물공학과) ;
  • 홍란희 (중앙대학교 의과대학 예방의학교실) ;
  • 강상모 (건국대학교 대학원 생물공학과)
  • Published : 2007.12.28
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Abstract

We studied the effects of genistein obtained from glycolysis of genistin, a kind of phytoestrogen present in soybeans, on cell proliferation and type I pN collagen synthesis in normal human dermal fibroblasts(NHDF). Cell proliferation was increased significantly with genistein treatment at 54-year aged NHDF. Genistein increased cell proliferation more strongly in cells form old doner than young doner. The senescence-associated ${\beta}$-galatosidase activity was decreased in NHDF from 77-year old doner with genistein treatment. Type I pN collagen synthesis was increased with genistein treatement in UVA treated and non-treated NHDF. The increasement of collagen synthesis was more effective in aged cells than young cells. Type I pN collagen synthesis was also increased with genistein treatment in collagen matrix culture with NHDF from sun-exposed and non-exposed skin from 54-year old doner. Genistein treatment inhibited MMP-1 synthesis in old NHDF but not in young NHDF. In conclusion, genistein may be a useful agent for preventing intrinsic aging as well as photoaging.

콩류에 다량 존재하는 제니스틴으로부터 얻을수 있는 제니스테인이 노화된 피부 섬유아세포의 활성과 피부의 주성분인 콜라겐의 합성 및 이의 주요한 분해 효소인 MMP-1의 합성에 어떤 영향을 미치는지 알아보고자 하였다. 제니스테인은 54세의 섬유아세포 증식을 유의하게 증가시켰으며, 이러한 세포증식 효과는 비타민 A와 유사하게 자연노화가 많이 진행된 세포에서 뚜렷하였다. 아울러 77세의 섬유아세포에서 제니스테인 처리에 의해 세포노화의 지표인 SA-${\beta}$-Gal 염색이 감소된 것을 확인하였다. 그리고 제니스테인은 섬유아세포에서 type I pN 콜라겐의 합성을 촉진하였고, UVA에 의해 콜라겐 합성이 매우 억제된 상황에서도 합성 증가 효과를 보였는데 이러한 현상은 자연노화가 많이 진행된 세포에서 더욱 효과적이었다. 콜라겐 매트릭스를 이용한 콜라겐 생합성 결과에서 제니스테인은 광노출부나 비노출 부위에서 얻은 모두의 세포에서 콜라겐 합성을 증가시킴을 알 수 있었으며, 이를 통해 제니스테인이 광노화 및 자연노화에 모두 효과적인 것으로 추측할 수 있다. 제니스테인은 젊은 피부의 세포에서 MMP-1의 합성에는 큰 영향을 미치지 않았으나, 노화된 피부의 세포에서 매우 증가되어 있는 MMP-1의 합성을 억제하였다. 이상의 결과에서 제니스테인은 광노화 뿐 아니라, 자연 노화에 의한 피부 주름을 예방하는데 유용한 물질이 될 것으로 사료된다.

Keywords

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