Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Comparison of osteogenic efficacy depending on implant preparation in autograft

자가골 이식에서 이식골편의 처리방법에 따른 골형성능력 비교

  • Lee, Jong-Il (Graduate School of Agricultural and Life Sciences, The University of Tokyo) ;
  • Song, Ha-Na (Seosin Animal Hospital) ;
  • Kim, Nam-soo (College of Veterinary Medicine, Chonbuk National University) ;
  • Choi, In-hyuk (College of Veterinary Medicine, Chonbuk National University)
  • 이종일 (동경대학교 농학생명과학대학원) ;
  • 송하나 (전주 서신동물병원) ;
  • 김남수 (전북대학교 수의과대학) ;
  • 최인혁 (전북대학교 수의과대학)
  • Accepted : 2007.02.02
  • Published : 2007.03.30
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Abstract

Bone graft had been investigated previously to restore bone defects in orthopedics. The purpose of this study was to investigate the efficacy on new bone formation in bone autografts by treatment of implants. Cortical bone autografts were transplanted to midshaft of diaphyseal fibulae of 9 rabbits which were divided in 3 groups according to the treatment method of implants. Cortical bone implants for graft were treated with 3 different methods; freezing, freeze-drying, defat-freezing. Autografts were achieved by cross-transplantation method to bilateral fibulae of the presented rabbits after implant treatment procedures. The grafted regions of fibulae of all groups had been radiographed biweekly for 16 weeks to observe new bone formation and union between donor and recipient bone in the grafted region. Bone alkaline phosphatase (BALP) in all groups was evaluated biweekly till the end of the experiment to determine osteoblast activities. Unions of the experimental grafted regions were observed at 83% (5 of 6 cases) of freezing, 17% (1 of 6 cases) of freeze-drying and 67% (4 of 6 cases) of defat-freezing autografts, respectively. BALP was increased over 100% after 2 weeks of graft procedures in all union cases (all cases in freezing group and in defat-freezing group, and 1 of 3 in freeze-drying group, respectively), then gradually decreased from 4 th week of graft to 16 th week. In non-union cases, there is no significant variation in BALP value until the end of experiment. It is speculated that defat-freezing method of treatments of implants is more safe to preserve the osteogenic ability in autograft than freeze-drying method.

Keywords

References

  1. 곽호윤. 요추부 후외측 유합술시 자가골 이식과 Tutoplast 가공 처리된 동종골 이식의 비교. 대한정형외과학회지 1998, 33, 1157-1163
  2. 김재도, 구자욱, 조명래, 장재호. 공격성 또는 악성 골종양에서의 동종골이식술. 대한정형외과학회지 2000, 35, 185-191
  3. 김준성, 윤근철. 토끼에서 냉동건조 동종연골 이식에 대한 실험적 연구. 대한성형외과학회지 1995, 22, 963-970
  4. 문명상, 우영균, 선두훈. 토끼의 장관골 결손부에 삽입된 탈회한 동종골 및 이종골의 골생성능. 대한정형 외과학회지 1991, 26, 1281-1288
  5. 박상원, 강오용, 정대철, 위대곤. 이종골 이식이 가토 장관골 결손부의 치유과정에 미치는 영향에 관한 실험적 연구. 대한정형외과학회지 1997, 32, 449-456
  6. 이동근. 동종골의 처리방법에 따른 골 치유 능력에 관한 실험적 연구. 대한구강악안면외과학회지 1997, 23, 43-62
  7. 이재은, 이동근, 엄인웅, 김영조, 김장연. 자가 탈회골의 조직반응에 관한 실험적 연구. 대한악안면성형재건외과학회지 1993, 15, 199-21
  8. 장익열. 동결건조골에 대한 소고. 대한정형외과학회지 1988, 23, 929-935
  9. 조덕연, 서재곤, 김응하, 장광. 장관골 골 결손에 시행한 동종골 이식 (증례 7례). 대한정형외과학회지 1993, 28, 1842-1850
  10. 최인혁, 김현경, 김남수, 佐佐木 伸雄. 개의 비유합 골절 Model에 있어서 동결건조골이식의 효과. 대한수의학회지 1996, 36, 495-511
  11. 최인혁, 이종일. 동결 건조한 산양 뼈의 개이식 효과. 한국임상수의학회지 1998, 15, 442-449
  12. Bauer TW, Muschler GF. Bone graft materials. An overview of the basic science. Clin Orthop Relat Res 2000, 371, 10-27 https://doi.org/10.1097/00003086-200002000-00003
  13. Behr W, Barnert J. Quantification of bone alkaline phosphatase in serum by precipitation with wheat-germ lectin: a simplified method and its clinical plausibility. Clin Chem 1986, 32, 1960-1966
  14. Brown MD, Malinin TI, Davis PB. A roentgenographic evaluation of frozen allografts versus autografts in anterior cervical spine fusions. Clin Orthop Relat Res 1976, 119, 231-236
  15. Burchardt H, Jones H, Glowczewskie F, Rudner C, Enneking WF. Freeze-dried allogeneic segmental cortical-bone grafts in dogs. J Bone Joint Surg Am 1978, 60, 1082-1090 https://doi.org/10.2106/00004623-197860080-00011
  16. Cheng JCY, Guo X, Law LP, Lee KM, Chow DHK, Rosier R. How does recombinant human bone morphogenetic protein-4 enhance posterior spinal fusion? Spine 2002, 27, 467-474 https://doi.org/10.1097/00007632-200203010-00006
  17. Cheung C. The future of bone healing. Clin Podiatr Med Surg 2005, 22, 631-641 https://doi.org/10.1016/j.cpm.2005.07.002
  18. Fishman WH. Perspectives on alkaline phosphatase isoenzymes. Am J Med 1974, 56, 617-650 https://doi.org/10.1016/0002-9343(74)90631-7
  19. Giannoudis PV, Dinopoulos H, Tsiridis E. Bone substitutes: an update. Injury 2005, 36 Suppl 3, S20-27
  20. Greenwald AS, Boden SD, Goldberg VM, Khan Y, Laurencin CT, Rosier RN. Bone-graft substitutes: facts, fictions, and applications. J Bone Joint Surg Am 2001, 83-A Suppl 2, 98-103
  21. Gruber R, Mayer C, Schulz W, Graninger W, Peterlik M, Watzek G, Luyten FP, Erlacher L. Stimulatory effects of cartilage-derived morphogenetic proteins 1 and 2 on osteogenic differentiation of bone marrow stromal cells. Cytokine 2000, 12, 1630-1638 https://doi.org/10.1006/cyto.2000.0760
  22. Habal MB. Different forms of bone grafts. In: Habal MB, Reddi AH (eds.). Bone Grafts & Bone Substitutes. pp. 6-8, Saunders, Philadelphia, 1992
  23. Heliotis M, Tsiridis EE. Fresh frozen bone in femoral impaction grafting: can developments in bone regeneration improve on this? Med Hypotheses 2001, 57, 675-678 https://doi.org/10.1054/mehy.2001.1376
  24. Henry WB, Wadsworth PL. Diaphyseal allografts in the repair of long bone fractures. J Am Anim Hosp Assoc 1989, 195, 1734-1744
  25. Hierholzer C, Sama D, Toro JB, Peterson M, Helfet DL. Plate fixation of ununited humeral shaft fractures: effect of type of bone graft on healing. J Bone Joint Surg Am 2006, 88, 1442-1447 https://doi.org/10.2106/JBJS.E.00332
  26. Hubble MJW. Bone transplantation. Curr Orthop 2001, 15, 199-205 https://doi.org/10.1054/cuor.2001.0179
  27. Iwata H, Sakano S, Itoh T, Bauer TW. Demineralized bone matrix and native bone morphogenetic protein in orthopaedic surgery. Clin Orthop Relat Res 2002, 395, 99-109 https://doi.org/10.1097/00003086-200202000-00010
  28. Johnson AL. Principles of bone grafting. Semin Vet Med Surg (Small Anim) 1991, 6, 90-99
  29. Jones KC, Andrish J, Kuivila T, Gurd A. Radiographic outcomes using freeze-dried cancellous allograft bone for posterior spinal fusion in pediatric idiopathic scoliosis. J Pediatr Orthop 2002, 22, 285-289 https://doi.org/10.1097/00004694-200205000-00003
  30. Miki T, Masaka K, Imai Y, Enomoto S. Experience with freeze-dried PGLA/HA/rhBMP-2 as a bone graft substitute. J Craniomaxillofac Surg 2000, 28, 294-299 https://doi.org/10.1054/jcms.2000.0161
  31. Pelker RR, Friedlaender GE. Biomechanical aspects of bone autografts and allografts. Orthop Clin North Am 1987, 18, 235-239
  32. Reddi AH. Bone morphogenetic proteins: from basic science to clinical applications. J Bone Joint Surg Am 2001, 83-A Suppl 1, S1-6
  33. Shand JM, Heggie AAC, Holmes AD, Holmes W. Allogeneic bone grafting of calvarial defects: an experimental study in the rabbit. Int J Oral Maxillofac Surg 2002, 31, 525-531 https://doi.org/10.1054/ijom.2002.0281
  34. Sinibaldi KR. Evaluation of full cortical allografts in 25 dogs. J Am Vet Med Assoc 1989, 194, 1570-1577
  35. Straalen JP, Sanders E, Prummel MF, Sanders GTB. Bone-alkaline phosphatase as indicator of bone formation. Clini Chim Acta 1991, 201, 27-34 https://doi.org/10.1016/0009-8981(91)90021-4
  36. Thoren K, Aspenberg P, Thorngren KG. Lipid extracted bank bone. Bone conduction and mechanical properties. Clin Orthop Relat Res 1995, 311, 232-246
  37. Trentz OA, Hoerstrup SP, Sun LK, Bestmann L, Platz A, Trentz OL. Osteoblasts response to allogenic and xenogenic solvent dehydrated cancellous bone in vitro. Biomaterials 2003, 24, 3417-3426 https://doi.org/10.1016/S0142-9612(03)00205-9
  38. Urist MR. Bone: formation by autoinduction. 1965. Clin Orthop Relat Res 2002, 395, 4-10 https://doi.org/10.1097/00003086-200202000-00002
  39. Wang JM, Oh JK, Kim DJ. Effect of the pedicle screw fixation on the anterior lumbar interbody fusion using the freeze-dried structural allograft. J Korean Orthop Assoc 1998, 33, 1569-1576