Beagle dogs parentage testing by using 22 ISAG microsatellite markers

  • Ji, Hye-jung (Department of Veterinary Medicine, Cheju National University) ;
  • Kim, Eun-hee (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Kyoung-kap (Department of Veterinary Medicine, Cheju National University) ;
  • Kang, Tae-young (Department of Veterinary Medicine, Cheju National University) ;
  • Lee, Joo-myoung (Department of Veterinary Medicine, Cheju National University) ;
  • Shin, Hyoung-doo (Department of Genetic Epidmiology, SNP Genetics) ;
  • Kim, Lyoung-hyo (Department of Genetic Epidmiology, SNP Genetics) ;
  • Yun, Young-min (Department of Veterinary Medicine, Cheju National University)
  • Accepted : 2007.11.26
  • Published : 2007.12.30

Abstract

The objective of the study was to establish routine parentage testing system in Beagle dogs using 22 ISAG (International Society for Animal Genetics) canine microsatellite markers (2005). Blood collections were obtained from a mother dog, 4 candidate father dogs and 3 offspring (n = 8). Genomic DNA samples were extracted from 8 Beagle dogs blood for PCR analysis. PCR products for the allele were analyzed by ABI 3130 DNA Sequencer and GeneScan (Ver 3.0) analysis and Genotyper (Ver. 2.1) software. The genetic relationship of mother and 3 offspring as well as one father dog among 4 candidate father dogs was confirmed by microsatellite allele analysis. The results of locus for amelogenin, which was designed for sexing, were matching with real gender among 8 Beagle dogs (female; 217/217 homozygosity, male; 179/217 heterozygosity). Twenty two ISAG microsatellite markers are useful the parentage test of Beagle dogs. In addition, amelogenin is an applicable marker to detecting real sex in dogs.

Keywords

References

  1. DeNise S, Johnston E, Halverson J, Marshall K, Rosenfeld D, McKenna S, Sharp T, Edwards J. Power of exclusion for parentage verification and probability of match for identity in American Kennel Club breeds using 17 canine microsatellite markers. Anim Genet 2004,35, 14-17 https://doi.org/10.1046/j.1365-2052.2003.01074.x
  2. Halverson J, Basten C. A PCR multiplex and database for forensic DNA identification of dogs. J Forensic Sci 2005, 50, 352-363
  3. Ichikawa Y, Takagi K, Tsumagari S, Ishihama K, Morita M, Kanemaki M, Takeishi M, Takahashi H. Canine parentage testing based on micro satellite polymorphisms. J Vet Med Sci 2001, 63, 1209-1213 https://doi.org/10.1292/jvms.63.1209
  4. Jouquand S, Priat C, Hitte C, Lachaume P, Andre C, Galibert F. Identification and characterization of a set of 100 tri- and dinucleotide microsatellites in the canine genome. Anim Genet 2000, 31, 266-272 https://doi.org/10.1046/j.1365-2052.2000.00642.x
  5. Lupke L, Distl O. Microsatellite marker analysis of the genetic variability in Hanoverian Hounds. J Anim Breed Genet 2005, 122, 131-139 https://doi.org/10.1111/j.1439-0388.2005.00501.x
  6. Mason IL. Man made animals; dogs. In: Clutton BJ (ed.). A Natural History of Domesticated Mammals. pp. 34-35, Cambridge University Press, Cambridge, 1993
  7. Muller S, Flekna G, Muller M, Brem G, Use of canine microsatellite polymorphisms in forensic examinations. J Hered 1999, 90, 55-56 https://doi.org/10.1093/jhered/90.1.55
  8. Oishi N, Maeda M, Makimura K, Sawagucbi T, Hayashiya M, Kubo T, Kano R, Hasegawa A, Kasahara M. Microsatellite polymorphism in Japanese mongrel dogs. J Vet Med Sci 2005, 67, 1055-1057 https://doi.org/10.1292/jvms.67.1055
  9. Ostrander EA, Mapa FA, Vee M, Rine J. One hundred and one new simple sequence repeat based markers for the canine genome. Mamm Genome 1995, 6, 192-195 https://doi.org/10.1007/BF00293011
  10. Schelling C, Gaillard C, Dolf G Genetic variability of seven dog breeds based on microsatellite markers, J Anim Breed Genet 2005, 122 (Suppl 1), 71-77 https://doi.org/10.1111/j.1439-0388.2005.00515.x
  11. Sundqvist AK, Bjornerfeldt S, Leonard JA, Hailer F, Hedhammar A, Ellegren H, Vila C. Unequal contribution of sexes in the origin of dog breeds. Genetics 2006, 172, 1121-1128 https://doi.org/10.1534/genetics.105.042358
  12. Zajc I, Mellersh C, Kelly EP, Sampson J. A new method of paternity testing for dogs, based on microsatellite sequences. Vet Rec 1994, 135, 545-547