Asian-Australasian Journal of Animal Sciences

  • 제20권11호
  • /
  • Pages.1651-1654
  • /
  • 2007
  • /
  • 1011-2367(pISSN)
  • /
  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
권호 표지
DOI QR코드

DOI QR Code

Associations of Polymorphisms in the Mx1 Gene with Immunity Traits in Large WhitexMeishan F2 Offspring

  • Li, X.L. (College of Animal Science and Technology, HeNan University of Science and Technology) ;
  • He, W.L. (College of Animal Science and Technology, HeNan University of Science and Technology) ;
  • Deng, C.Y. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University) ;
  • Xiong, Y.Z. (Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agriculture University)
  • 투고 : 2007.03.05
  • 심사 : 2007.06.10
  • 발행 : 2007.11.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The mouse myxovirus resistance protein 1 (Mx1) is known to be sufficient to confer resistance to influenza viruses, and the gene encoding Mx1 is, therefore, an interesting candidate gene for disease resistance in farm animals. The porcine Mx1 gene has already been identified and characterized based on its homology with mouse Mx1; the full-length coding region of the pig Mx1 gene spans 2,545 bp (M65087) and is organized into 17 exons compared with the human ortholog mRNA. In this study, the exons 9, 10 and 11 and introns 6 and 9 of the porcine Mx1 gene were cloned and sequenced. Two SNPs were identified in exons 9, 10 and 11 but none of the SNPs led to an amino acid exchange, and the other eleven variants were detected in introns 6 and 9, respectively. Differences in allele frequency between Meishan and other pig breeds were observed within intron 6, of which an $A{\rightarrow}G$ substitution at position 371 was detected as an SnaBI PCR-RFLP. The association analysis using the Large White${\times}$Meishan $F_2$ offspring suggested that the Mx1 genotype was associated with variation in several immunity traits that are of interest in pig breeding. However, further investigations in more populations are needed to confirm the above result.

키워드

참고문헌

  1. Arnheiter, H., S. Skuntz, M. Noteborn, S. Chang and E. Meier. 1990. Transgenic mice with intracellular immunity to influenza virus. Cell 62:51-61. https://doi.org/10.1016/0092-8674(90)90239-B
  2. Chieux, V., W. Chehadeh, J. Harvey, O. Haller, P. Wattre and D. Hober. 2001. Inhibition of coxsackievirus B4 replication in stably transfected cells expressing human MxA protein. Virol. 283:84-92. https://doi.org/10.1006/viro.2001.0877
  3. Gordien, E., O. Rosmorduc, C. Peltekian, F. Garreau, C. Brechot and D. Kremsdorf. 2001. Inhibition of hepatitis B virus replication by the interferon-inducible MxA protein. J. Virol. 75:2684-2691. https://doi.org/10.1128/JVI.75.6.2684-2691.2001
  4. Haller, O., M. Frese and G. Kochs. 1998. Mx proteins: mediators of innate resistance to RNA viruses. Rev. Sci. Technical. 17:220-230. https://doi.org/10.20506/rst.17.1.1084
  5. Horisberger, M. A., G. K. McMaster, H. Zeller, M. G. Wathelet, J. Dellis and J. Content. 1990. Cloning and sequence analyses of cDNAs for interferon- and virus-induced human Mx proteins reveal that they contain putative guanine nucleotide-binding sites: functional study of the corresponding gene promoter. J. Virol. 64:1171-1181.
  6. Horisberger, M. A., P. Staeheli and O. Haller. 1983. Interferon induces a unique protein in mouse cells bearing a gene for resistance to influenza virus. Proc. Nati. Acad. Sci. USA. 80:1910-1914. https://doi.org/10.1073/pnas.80.7.1910
  7. Landis, H., A. Simon-Jodicke, A. Klati, C. Di Paolo, J. Schnorr, S. Schneider-Schaulies, H. P. Hefti and J. Pavlovic. 1998. Human MxA protein confers resistance to Semliki forest virus and inhibits the amplification of a Semliki forest virus-based replicon in the absence of viral structural proteins. J. Virol. 72:1516-1522.
  8. Liu, B. H. 1998. Statistical Genomics: Linkage, Mapping and QTL Analysis. CRC Press, LLC. 404-409.
  9. Morozumi, T., C. Sumantri, E. Nakajima, E. Kobayashi, A. Asano, T. Oishi, T. Mitsuhashi, T. Watanabe and N. Hamasima. 2001. Three types of polymorphisms in exon 14 in porcine Mx1 gene. Biochem. Genet. 39:251-260. https://doi.org/10.1023/A:1010230715605
  10. Nakayama, M., K. Nagata, A. Kato and A. Ishihama. 1991. Interferon-inducible mouse Mx1 protein that confers resistance to influenza virus is GTPase. J. Biol. Chem. 266:21404-21408.
  11. Obar, R. A., C. A. Collins, J. A. Hammarback, H. S. Shpetner and R. B. Vallee. 1990. Molecular cloning of the microtubuleassociated mechanochemical enzyme dynamin reveals homology with a new family of GTP-binding proteins. Nature 347:256-261. https://doi.org/10.1038/347256a0
  12. Rothman, J. H., C. K. Raymond, T. Gilbert, P. J. O'Hara and T. H. Stevens. 1990. A putative GTP binding protein homologous to interferon-inducible Mx proteins performs an essential function in yeast protein sorting. Cell 61:1063-1074. https://doi.org/10.1016/0092-8674(90)90070-U
  13. Shimizu, M., I. C. Pan and W. R. Hess. 1977. Celluar immunity demonstrated in pigs infected with African fever virus. Am. J. Veter. Res. 38:27-33.
  14. Staeheli, P., O. Haller, W. Boll, J. Lindenmann and C. Weissmann. 1986. Mx protein: constitutive expression in 3T3 cells transformed with cloned Mx cDNA confers selective resistance to influenza virus. Cell 44:147-158. https://doi.org/10.1016/0092-8674(86)90493-9
  15. Staeheli, P., F. Pitossi and J. Pavlovic. 1999. Mx proteins: GTPases with antiviral activity. Trends Cell Biol 3:268-272.
  16. Wang, T., C. Liu, Y. ZH. Xiong, C. Y. Deng, B. Zuo, H. T. Xie and D. Q. Xu. 2007. Isolation and cloning of porcine SLC27A2 gene and detection of its polymorphism asscciated with growth and carcass traits. Asian-Aust. J. Anim. Sci. 20(8):1169-1173. https://doi.org/10.5713/ajas.2007.1169
  17. Wang, W. J., K. H. Ouyang, X. F. Su, M. S. Xu and X. C. Shangguan. 2006. Polymorphism of insulin-like growth factor 1 receptor gene in 12 pig breeds and its relationship with pig performance traits. Asian-Aust. J. Anim. Sci. 19(11):1541-1545. https://doi.org/10.5713/ajas.2006.1541
  18. Xiong, Y. Z. and C. Y. Deng. 1999. Principle and Method of Swine Testing. Chinese Agriculture Press, Beijing.
  19. Zhang, F. W., H. C. Cheng, C. Y. Deng, Y. Z. Xiong, F. E. Li and M. G. Lei. 2006. cDNA cloning, tissue expression and association of porcine pleiomorphic adenoma gene-like 1(PLAG1) gene with carcass traits. Asian-Aust. J. Anim. Sci. 19(9):1257-1261. https://doi.org/10.5713/ajas.2006.1257
  20. Zhang, Z. B., M. G. Lei, C. Y. Deng, Y. ZH. Xiong, B. Zuo and F. E. Li. 2005. Lipoprotein lipase gene and productive traits in pig resource family. Asian-Aust. J. Anim. Sci. 18:458-462. https://doi.org/10.5713/ajas.2005.458