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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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A Novel Role of Classical Swine Fever Virus Erns Glycoprotein in Counteracting the Newcastle Disease Virus (NDV)-mediated IFN-β Induction

  • Xia, Yan-Hua (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Chen, Liu (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Pan, Zi-Shu (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Zhang, Chu-Yu (State Key Laboratory of Virology, College of Life Sciences, Wuhan University)
  • Published : 2007.09.30
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Abstract

$E^{rns}$ is an envelope glycoprotein of classical swine fever virus (CSFV) and has an unusual feature of RNase activity. In the present study, we demonstrate that $E^{rns}$ counteracts Newcastle disease virus (NDV)-mediated induction of IFN-$\beta$. For this purpose, $E^{rns}$ fused to the enhanced green fluorescent protein (EGFP) was transiently expressed in porcine kidney 15 (PK15) cells. In luciferase activity assay, $E^{rns}$-EGFP was found to prevent IFN-$\beta$ promoter-driven luciferase expression and block the induction of IFN-$\beta$ promoter mediated by NDV in a dose-dependent manner. Through IFN-specific semi-quantitative RT-PCR detection, obvious decrease of IFN-$\beta$ mRNA in NDV-infected PK15 cells was observed in the presence of $E^{rns}$-EGFP. In contrast, EGFP alone showed none of this block capacity. In addition, $E^{rns}$-EGFP mutations with RNase inactivation were also found to block NDV-mediated induction of IFN-$\beta$. These evidences establish a novel function for CSFV $E^{rns}$ glycoprotein in counteraction of the IFN-$\beta$ induction pathway.

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References

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