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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Isolation and Characterization of Two Korean Mistletoe Lectins

  • Kang, Tae-Bong (School of Food and Biosciences, Handong Global University) ;
  • Song, Seong-Kyu (School of Food and Biosciences, Handong Global University) ;
  • Yoon, Taek-Joon (Bio-Food and Drug Research Center, Konkuk University) ;
  • Yoo, Yung-Choon (Department of Microbiology, College of Medicine, Konyang University) ;
  • Lee, Kwan-Hee (School of Food and Biosciences, Handong Global University) ;
  • Her, Erk (Department of Immunology, College of Medicine, Konkuk University) ;
  • Kim, Jong-Bae (School of Food and Biosciences, Handong Global University)
  • Published : 2007.11.30
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Abstract

Two isolectins (KML-IIU and the KML-IIL) were individually isolated from the previously reported Korean mistletoe lectin, KML-C, by using an immunoaffinity column. Molecular weights of the KML-IIU and the KML-IIL were 64 kDa and 60 kDa respectively. Both of the lectins were composed of heterogeneous A and B subunits linked with a disulfide bond, and showed the same carbohydrate-binding specificities for Gal and GalNAc. However, they are different not only in biophysical properties (glycosylation and amino acid compositions) but also bioactivities (cell killing and cytokine induction). The KML-IIL showed 17-145 times stronger in cytotoxicities to various human and mouse cancer cell lines than the KML-IIU. The KML-IIL also induced TNF-$\alpha$ secretion from mouse peritoneal macrophages 4.5 times better than the KML-IIU. The results demonstrated isolectins in Korean mistletoe were varied in bioactivities and the KML-IIL may be developed as an anti-cancer agent.

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References

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