Asian-Australasian Journal of Animal Sciences

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Improved Expression of Muscle-derived Growth Hormone Releasing Hormone from ${\alpha}$-Skeletal-muscle Actin Enhancer/Cytomegalovirus Hybrid Enhancer/Promoter

  • Gong, Xia (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Meng, Qingyong (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Jin, Weiwu (State Key Laboratory for Agrobiotechnology, China Agricultural University) ;
  • Li, Ning (State Key Laboratory for Agrobiotechnology, China Agricultural University)
  • Received : 2006.04.05
  • Accepted : 2006.06.26
  • Published : 2007.05.01
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Abstract

Growth hormone-releasing hormone (GHRH), a hypothalamic neuropeptide can stimulate the growth hormone secretion from the anterior pituitary. In this study, a porcine GHRH expression plasmid pHC-GHRH was used to enhance growth performance through ectopic expressions in muscle tissues of rats. Rats injected with the plasmid of pHC-GHRH and pCMV-GHRH exhibited cumulative weight gains 6.4% and 1% greater than controls. During a 5-day period, significant weight gain differences were observed as follows compared with that of control: during 5-10 days post-injection (DPI) period, the group pHC-GHRH on average 14.5% heavier than controls, $40.73{\pm}0.88$ g vs. $35.57{\pm}1.23$ g (p = 0.0023); during 10-15 DPI period, the group pHC-GHRH on average 13.6% heavier than controls, $37.49{\pm}2.85$ g vs. $33.00{\pm}1.56$ g (p = 0.0146); during 15-20 DPI period, the group pHC-GHRH on average 17.8% heavier than controls, $25.64{\pm}1.39$ g vs. $21.77{\pm}1.27$ g (p<0.05). In addition, plasmids-treated rats maintained higher serum IGF-I than controls. Significant differences of IGF-I were observed on 13 DPI and on 40 DPI in pHC-GHRH group compared with that of controls. This was accomplished through the use of an improved expression cassette that included the cytomegalovirus (CMV) immediate early enhancer/promoter in combination with a 1.5-kilobase portion of porcine ${\alpha}$-skeletal muscle actin promoter.

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References

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