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Function and Oligomerization Study of the Leucine Zipper-like Domain in P13 from Leucania separata Multiple Nuclear Polyhedrosis Virus

  • Du, Enqi (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Yao, Lunguang (Nanyang-Rothamsted Joint Laboratory for Insect Biology, Nanyang Normal University) ;
  • Xu, Hua (Nanyang-Rothamsted Joint Laboratory for Insect Biology, Nanyang Normal University) ;
  • Lu, Songya (State Key Laboratory of Virology, College of Life Sciences, Wuhan University) ;
  • Qi, Yipeng (Nanyang-Rothamsted Joint Laboratory for Insect Biology, Nanyang Normal University)
  • Published : 2007.03.31
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Abstract

The p13 gene is uniquely present in Group II nucleopolyhedroviruses (NPVs) and some granuloviruses, but not in Group I NPVs. p13 gene was first described by our laboratory in Leucania separatamultiple nuclear polyhedrosis virus (Ls-p13) in 1995. However, the functions of Ls-P13 and of its homologues are unknown. When Ls-p13 was inserted into Autographa californica nucleopolyhedrovirus, a Group I NPV, polyhedra yield was inhibited. However, this inhibition was prevented when the leucine zipper-like domain of Ls-p13 was mutated. To determine the cause of this marked difference between Ls-P13 and leucine zipper mutated Ls-P13 (Ls-P13mL), oligomerization and secondary structure analyses were performed. High performance liquid chromatography and yeast two-hybrid assays indicated that neither Ls-P13 nor Ls-P13mL could form oligomers. Informatics and circular dichroism spectropolarimetry results further indicated marked secondary structural differences between Ls-P13 and Ls-P13mL. The LZLD of Ls-P13 has two extended heptad repeat units which form a hydrophobic surface, but it is short of a third hydrophobic heptad repeat unit for oligomerization. However, the mutated LZLD of Ls-P13mL lacks the above hydrophobic surface, and its secondary structure is markedly different. This difference in its secondary structure may explain why Ls-P13mL is unable to inhibit polyhedra yield.

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References

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