Korean Journal of Veterinary Research (대한수의학회지)

The Korean Society of Veterinary Science (대한수의학회)
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Immunogenicity of the recombinant Pasteurella multocida toxin for development of subunit vaccine against swine atrophic rhinitis

돼지 위축성 비염 단위 백신 개발을 위한 재조합 파스튜렐라 독소 단백질의 면역원성 검정

  • Lee, Jeongmin (Institute of Life Science and Technology, Sungkyunkwan University)
  • 이정민 (성균관대학교 생명공학연구소)
  • Accepted : 2006.10.10
  • Published : 2007.03.30
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Abstract

Pasteurella multocida Pasteurella multocida toxin (PMT) is a causal pathogenin atrophic rhinitis in pigs. To investigate the protective immunity and vaccination effect of recombinantPMT, the gene for PMT was isolated from the infective P. multocida D:4. The 2.3 kb XhoI/PstI fragment(PMT2.3) of PMT gene was expressed in E. coli BL21 (DE3) using the induced expression vector system.The recombinant protein of PMT2.3 having molecular weight of 84 kDa was purified by Ni-afinitycolumn chromatography. The PMT2.3 raised slightly less anti-PMT antibody titer than formalin-killedwhole cel, however, it showed more protective imunity against P. multocida D:4 infection in vaccinationand chalenge.

Keywords

References

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