Treatment of Partial Thickness Skin Defect with Cultured Allogenic Keratinocytes (Kaloderm®)

동종유래각질세포(Cultured Allogenic Keratinocytes, Kaloderm®)를 이용한 부분층 피부 결손의 치료

  • Seo, Sang Won (Department of Plastic and Reconstructive Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine,) ;
  • Chang, Choong Hyun (Department of Plastic and Reconstructive Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine,) ;
  • Cho, Min Su (Department of Plastic and Reconstructive Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine,) ;
  • Hong, Yoon Gi (Department of Plastic and Reconstructive Surgery, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine,) ;
  • Jeon, Sae Wha (Tego Science Inc.)
  • 서상원 (성균관대학교 의과대학 강북삼성병원 성형외과학교실) ;
  • 장충현 (성균관대학교 의과대학 강북삼성병원 성형외과학교실) ;
  • 조민수 (성균관대학교 의과대학 강북삼성병원 성형외과학교실) ;
  • 홍윤기 (성균관대학교 의과대학 강북삼성병원 성형외과학교실) ;
  • 전세화 (테고 사이언스(주))
  • Received : 2006.11.17
  • Accepted : 2007.03.21
  • Published : 2007.06.30

Abstract

Purpose: Grafting with autograft skin remains the most effective method for treating skin defects. When insufficient donor sites are present or patients are afraid of the operation, a skin graft is impossible. Cultured allogenic keratinocytes speed wound healing by providing cover and by producing growth factors and extracellular matrix protein. We report an application of cultured allogenic keratinocytes ($Kaloderm^{(R)}$, Tegoscience, Seoul, Korea) in the treatment of an acute partial thickness skin defect. Methods: From March 2005 to January 2006, 20 patients with a partial thickness skin defect were treated with cultured allogenic keratinocytes. The wound was covered with a sheet of cultured allogenic keratinocytes and ointment with $Bactigras^{(R)}$ gauze. The wound was inspected every two or three days. We regarded completion of epithelialization as wound healing. Results: The mean period between time of injury and time of $Kaloderm^{(R)}$ application was 7.5 days. The time taken from application of $Kaloderm^{(R)}$ to complete closure of the wounds was 7.2 days. Conclusion: In view of the favorable outcome, cultured allogenic keratinocytes are safe and effective biologic dressing materials for use in the treatment of open wounds.

Keywords

References

  1. Kim SH, Lee JH, Lee DE: Dressing materials in the STSG donor site management: a comparative study. J Korean Soc Plast Reconstr Surg 2004;31:71-5
  2. Jeon JW, Kim DH, Hur J, Chun W, Jang WY, Kim JH: Clinical practice of glycerol preserved xenograft. J Korean Soc Burn 2004;7:73-8
  3. Tark KC, Rah DK, Lee YH: Clinical study on the effect of $Biobrane^{\circle}R$ in burn wounds. J Korean Soc Plast Reconstr Surg 1987;14:201-10
  4. Scott CH, John PH, Hiromasa Y, Athina G, Suzan D, Jeffrey AF, Anthony AM: Immunogenicity of cultured keratinocyte allografts deficient in major histocompatibility complex antigens. J Trauma 1998;45:25-34 https://doi.org/10.1097/00005373-199807000-00005
  5. Nigel C, Harshad AN, Colin JG, Irene ML: Acute rejection of cultured keratinocyte allografts in nonimmunosuppressed pigs. Transplantation 1991;52:918-21 https://doi.org/10.1097/00007890-199111000-00034
  6. Richard F, Frances P, Ihsan H, Cindy L, Irwin AS: Keratinocyte allografts accelerate healing of split-thickness donor sites: applications for improved treatment of burns. J Burn Care Rehabil 1993;14:148-54 https://doi.org/10.1097/00004630-199303000-00004
  7. Hiroshi U, Hiroshi T, Tetsuya K, Masutaka F: Cytokines and chemokines in the epidermis. J Dermatol Sci 2000;24:S29-S38 https://doi.org/10.1016/S0923-1811(00)00138-9
  8. Koji H: Regulation of keratinocyte function by growth factors. J Dermatol Sci 2000;24:S46-S50 https://doi.org/10.1016/S0923-1811(00)00141-9
  9. Whang KK, Kim MJ, Song WK, Cho S: Comparative treatment of giant congenital melanocytic nevi with curettage or Er: YAG laser ablation alone versus with cultured epithelial autografts. Dermatol Surg 2005;31:1660-7 https://doi.org/10.2310/6350.2005.31305