Archives of Plastic Surgery

Korean Society of Plastic and Reconstructive Surgeons (대한성형외과학회)
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Effect of Heparin on Expression of mRNA of MMP 1,2,9 in Adult Rat Wound

헤파린이 쥐의 창상치유에서 MMP-1,2,9 mRNA의 발현에 미치는 영향

  • Kim, Han Koo (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University) ;
  • Hong, Jung Soo (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University) ;
  • Kim, Sae Hwan (DF Plastic Surgery Clinic) ;
  • Lee, Mi Kyoung (Department of Pathology, College of Medicine, Chung-Ang University) ;
  • Kim, Seung Hong (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University) ;
  • Kim, Woo Seob (Department of Plastic and Reconstructive Surgery, College of Medicine, Chung-Ang University)
  • 김한구 (중앙대학교 의과대학 성형외과학교실) ;
  • 홍정수 (중앙대학교 의과대학 성형외과학교실) ;
  • 김세환 (DF성형외과) ;
  • 이미경 (중앙대학교 의과대학 진단검사의학과) ;
  • 김승홍 (중앙대학교 의과대학 성형외과학교실) ;
  • 김우섭 (중앙대학교 의과대학 성형외과학교실)
  • Received : 2006.08.14
  • Published : 2007.03.10
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Abstract

Purpose: This study was to investigate how the heparin, which has been known to induce neovascularization by MMP in the infarcted tissue of the myocardium, had influence on the expression of mRNA of MMP 1,2,9 of the skin wound of rat. Methods: Full depth skin wounds were created on the dorsum of Sprague-Dawley 60 rats. The experimental rats were divided into two groups according to the concentration of heparin($100{\mu}g/ml$ in 20, $300{\mu}g/ml$ in 20). Heparin soaked gelatin sponges in different concentration were inserted into the pocket of experimental rats and the wounds were closed. Normal saline soaked gelatin sponges were used in control rats. Wounds were harvested at 48 and 72 hours after closure. We performed histologic study in H-E stain. RNA was isolated from the harvested tissue and then real time polymerase chain reaction was performed to determine the gene expression of MMP-1,2,9. Results: We observed that inflammatory cell decreased in heparin soaked group and heparin increased the expression of MMP-1,9 mRNA of dorsal wound of rat at 72 hours (p < 0.05). Conclusion: This result suggest that heparin may be used inducing another factor inducing scarless wound healing by increasing MMP.

Keywords

Acknowledgement

Supported by : 중앙대학교

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