Molecular & Cellular Toxicology

The Korean Society of Toxicogenomics and Toxicoproteomics (대한독성유전 단백체학회)
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Growth of Budding Yeasts under Optical Trap

  • Im, Kang-Bin (Department of Physics, Hanyang University) ;
  • Kim, Hyun-Ik (Department of Physics, Hanyang University) ;
  • Kim, Soo-Ki (Department of Microbiology, Institute of Basic Medical Sciences, Yonsei University, Wonju College of Medicine) ;
  • Kim, Chul-Geun (Department of Life Science, Hanyang University) ;
  • Oh, Cha-Hwan (Department of Physics, Hanyang University) ;
  • Song, Seok-Ho (Department of Physics, Hanyang University) ;
  • Kim, Pill-Soo (Department of Physics, Hanyang University)
  • Published : 2007.03.31
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Abstract

Optic tweezer is powerful tool to investigate biologic cells. Of eukaryotic cells, it was poorly documented regarding to optic trapping to manipulate yeasts. In preliminary experiment to explore yeast biology, interferometric optical tweezers was exploited to trap and manipulate budding yeasts. Successfully, several budding yeasts are trapped simultaneously. We found that the budding direction of the daughter cell was almost outward and the daughter cell surrounded by other yeasts grows slowly or fail to grow. Thus it was assumed that neighboring cells around budding yeast may be critical in budding and the growth of daughter cells. This is first report pertaining to the pattern of yeast budding under the optical trap when multiple yeasts were trapped.

Keywords

References

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