Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Effects of Korean red ginseng and herb extracts mixture (KTNG0345) on procollagen biosynthesis and matrix metalloproteinase-1 activity in human dermal fibroblast

홍삼 생약 복합물(KTNG0345)이 섬유아세포의 Procollagen 생합성과 Matrix metalloproteinase-1(MMP-1) 활성에 미치는 영향

  • So, Seung-Ho (Health Food Research Group, KT&G Central Research Institute) ;
  • Lee, Seong-Kye (Health Food Research Group, KT&G Central Research Institute) ;
  • Hwang, Eui-Il (Health Food Research Group, KT&G Central Research Institute) ;
  • Koo, Bon-Suk (Health Food Research Group, KT&G Central Research Institute) ;
  • Han, Gyeong-Ho (Health Food Research Group, KT&G Central Research Institute) ;
  • Kim, Na-Mi (Health Food Research Group, KT&G Central Research Institute)
  • 소승호 (KT&G 중앙연구소 건강식품연구소) ;
  • 이성계 (KT&G 중앙연구소 건강식품연구소) ;
  • 황의일 (KT&G 중앙연구소 건강식품연구소) ;
  • 구본석 (KT&G 중앙연구소 건강식품연구소) ;
  • 한경호 (KT&G 중앙연구소 건강식품연구소) ;
  • 김나미 (KT&G 중앙연구소 건강식품연구소)
  • Published : 2007.12.31
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Abstract

Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

본 실험은 홍삼, 사상자, 산수유를 이용하여 주름 예방 효과가 있는 건강기능 식품을 개발하기 위한 기초자료로 활용하기 위하여 개별추출물과 이들의 혼합물 WM1, WM3 (KTNG0345)에 대하여 사람의 섬유아세포를 이용한 세포증식, 콜라겐 생합성 정도, MMP-1 효소활성, 항산화 활성을 조사하였다. 사용된 시료는 $1{\sim}10\;{\mu}g/ml$의 농도로 첨가하여 세포를 배양했을 때 $83{\sim}100%$의 세포증식율을 나타내어 세포 독성을 나타내지 않았으며, 콜라겐 생합성 정도는 대조군이 474.8 ng/ml이었고 retinoic acid는 568.3 ng/ml이었으며, 5 ug/ml의 농도를 기준으로 할 때 홍삼은 533.9ng/ml, 사상자는 539.3 ng/ml, 산수유는 514.1 ng/ml이었다. 홍삼과 생약을 68:232의 비율로 혼합한 WM1은 474.6 ng/ml이었고 홍삼과 생약을 136:164의 비율로 혼합한 WM3는 561.4 ng/ml로 증가하였다. MMP-1활성은 10 ug/ml의 농도에서 홍삼(ER)은 31.9 ng/ml, 사상자(WT)는 32.85 ng/ml, 산수유(WC)는 32.0 ng/ml, WM1은 31.3 ng/ml이었고 WM3(KTNG0345)는 28.85 ng/ml으로 대조군의 88% 수준으로 감소하였다. SOD활성에서는 1000 ug/ml의 농도로 처리하였을 때 65-97% 항산화활성을 나타내었다. 이들의 결과를 종합해 보면 홍삼과 사상자, 산수유 및 이들 혼합물은 human dermal fibroblas에 대하여 세포 독성을 나타내지 않으면서 콜라겐 생합성을 촉진하고 MMP-1의 활성을 억제하였으며 홍삼과 생약 엑기스를 136:164의 비율로 혼합했을 때 그 효과가 증진되었다. WM3 (KTNG0345)는 지표성분으로서 ginsenoside-$Rb_1$, torilin, loganin을 각각 10.85 mg/g, 0.128 mg/g, 3.92 mg/g 함유하였다.

Keywords

References

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