Journal of Ginseng Research

The Korean Society of Ginseng (고려인삼학회)
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Changes of phenolic acid contents and radical scavenging activities of ginseng according to steaming times

수삼의 증숙 횟수에 따른 페놀산 함량 변화와 라디칼 소거활성

  • Published : 2007.12.31
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Abstract

This study was conducted to investigate the contents of the total phenolic compounds, and DPPH, ABTS radical scavenging activities of phenolic acid fractions of ginseng according to steaming times. Also the individual phenolic acid compositions and contents were analyzed by GC. The contents of the total phenolic compounds proportionally increased from 0.530 to 2.893% according to steaming times. Phenolic acid fractions were separated according to bound types, and the insoluble bound form fraction showed the highest contents followed by ester form fraction and free form fraction. The total contents of these three fractions (1.031-1.416%) were not significantly influenced by steaming times. Salicylic, cinamic, p-hydroxybenzoic, gentisic, vanillic, syringic, caffeic, ferolic acid were found in each fraction, and gentisic and ferolic acid were the major phenolic acid. Each phenolic acid fraction showed over 50% of DPPH and ABTS radical scavenging activities. There were no differences between the phenolic acid fractions according to binding types. Free radical scavenging activities were affected by a number of steaming times and augmented as steaming times increased.

본 연구는 증숙 횟수에 따른 인삼의 총페놀 화합물 함량과 페놀산 획분의 DPPH, ABTS 라디칼 소거활성을 시험하고, 개별 페놀산 조성과 함량을 GC로 분석하였다. 총페놀 함량은 0.53-2.89%로 증숙 횟수에 비례하여 증가하였다. 결합 형태에 따른 페놀산 획분의 함량은 결합형 >에스테르형>유리형 순으로 나타났으며, 이들 세 획분의 총량 (1.03-1.41%)은 증숙횟수에 영향을 받지 않았다. 각 획분에서 salicylic, cinnamic, p-hydroxybenzoic, gentisic, vanillic, syingic, caffeic, ferolic acid가 확인되었으며, 이 중 gentisic acid와 ferulic acid 가 주된 페놀산으로 50% 이상을 차지하였다. 각 페놀산 획분은 50% 이상의 DPPH와 ABTS 라디칼 소거활성을 보였으며, 결합형태에 따른 페놀산 획분 간에는 큰 차이가 없었다. 반면 각 획분별로 증숙 횟수에 따라 라디칼 소거활성이 차이를 보였으며, 증숙 횟수가 증가할수록 라디칼 소거활성도 증가하는 경향을 보였다.

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References

  1. Mock, C. K., Song, K. T., Lee, S. K., Na, Y. J., Park, J. H., Kwon, Y. A. and Lee, S. J. : Optimization of roasting process as pretreatment for extraction of omija(Schizandra chinensis Baillon). Korean J. Food Sci. Technol. 33, 333-337 (2001)
  2. Hong, M. J., Lee, G. D., Kim, H. K. and Kwon J. H. : Changes in functional and sensory properites of chicory roots. Korean J. Food Sci. Technol. 30, 413-418 (1998)
  3. Kim, J. K., Ha, W. D., Ha, J. H., Moon, K. D. and Chung, S. K. : Changes of volatiles flavor components on roasting conditions in Cassia tora seeds. Korean J. Food Sci. Technol. 27, 736-741 (1995)
  4. Hwang, I. G., Woo, K. S., Kim, T. M., Kim, D. J., Yang, M. H. and Jeong, H. S. : Change of physicochemical characteristics of Korean pear(Pyrus pyrifolia Nakai) juice with heat treatment conditions. Korean J. Food Sci. Technol. 38, 342-347 (2006)
  5. Okuda, H. : Medicinal Korean ginsneng 95. Kyoritsu Pub. Co., Tokyo, Japan, P. 233 (1995)
  6. Shin, J. G., Park, J. W., Pyo, J. K., Kim, M. S. and Chung, M. H. : Protective effects of a ginseng component, maltol(2- methyl-3-hydroxy-4-pyron) against tissue damages induced by oxygen radicals. Korean J. Ginseng Sci. 14, 187-190 (1990)
  7. Kwak, Y. S., Shin, H. J., Song, Y. B., Kyung, J. S., Wee, J. J. and Park, J. D. : Effect of oral administration of red ginseng acidic polysaccharide(RGAP) on the tumor growth inhibition. J. Ginseng Res. 29, 176-181 (2005) https://doi.org/10.5142/JGR.2005.29.4.176
  8. Zwart, L. L., Meerman, J. H. N., Commandeur, N. M. and Verneulen, N. P. E. : Biomarkers of free radical damage applications in experimental amimals and in humans. Free Rad. Biol. & Med. 26, 202-226 (1999) https://doi.org/10.1016/S0891-5849(98)00196-8
  9. Lodovici, M., Guglielmi, F., Meoni, M. and Dolara, P. : Effect of natural phenolic acids on DNA oxidation in vitro. Food and Chem. Toxicol. 39, 1205-1210 (2001) https://doi.org/10.1016/S0278-6915(01)00067-9
  10. Choi, C. S., Kim, K. I., Hong, H. D., Choi, S. Y., Lee, Y. C., Kim, K. T., Rho, J., Kim, S. S. and Kim, Y. C. : Phenolic acid composition and antioxidative activity of white ginseng( Panax ginseng C.A. Meyer) J. Ginseng Res. 30, 22-30 (2006) https://doi.org/10.5142/JGR.2006.30.1.022
  11. Jung, M. Y., Jeon, B. S. and Bock, J. Y. : Free, esterified, and insoluble-bound phenolic acids in white and red Korean ginseng( Panax ginseng C.A. Meyer). Food Chem. 79, 105-111 (2000) https://doi.org/10.1016/S0308-8146(02)00185-1
  12. Han, B. H., Park, M. H., Han, Y. N. and Shin, S. C. : Studies on the antioxidant components of Korean ginseng(), Antifatigue active components. Yakhak Hoeji 28, 231-235 (1984)
  13. Kim Y, K., Guo, Q. and Packer, L. : Free radical scavenging activity of red ginseng aqueous extracts. Toxicol. 172, 149- 156 (2002) https://doi.org/10.1016/S0300-483X(01)00585-6
  14. Kim, D. J. and Chang, C. C. : The effects of red ginseng extracts on antioxidant enzyme activities and lipid peroxidation of the kidney in $\gamma$-postirradiated mice. Korean J. Ginseng Sci. 18, 25-31 (1994)
  15. Kim, S. H. and Park, K. S. : Effects of panax ginseng extract on lipid metboilism in humans. Pharmacol. Res. 48, 511-513 (2003) https://doi.org/10.1016/S1043-6618(03)00189-0
  16. AOAC. : Offical methods of analysis. 15th ed. Association of official analytical chemists. Washington DC. Cd 8-35 (1990)
  17. Krygier, K., Sosulski, F. and Lawrence, H. : Free, esterified and insoluble-bound phenolic acids. 1. Extract and purification procedure. J. Agric. Food Chem. 30, 330-334 (1982) https://doi.org/10.1021/jf00110a028
  18. Choi, C. S., Kim, K. I., Hong, H. D., Choi, S. Y., Lee, Y. C., Kim, K. T., Rho, J., Kim, S. S. and Kim, Y. C. : Phenolic acid composition and antioxidative activity of white ginseng (Panax ginseng, C.A. Meyer). J. Ginseng Res. 30, 22-30 (2006) https://doi.org/10.5142/JGR.2006.30.1.022
  19. Blois, M. S. : Antioxidant determination by the use of a stable free radical. Nature. 181, 1199-1200 (1958) https://doi.org/10.1038/1811199a0
  20. Van den Berg, R., Haenen, G.R., Van den Berg, H. and Bast, A. : Applicability of an improved Trolox equivalent antioxidant capacity(TEAC) assay for evaluation of antioxidant capacity measurements of mixtures. Food Chem. 66, 511- 517 (1999) https://doi.org/10.1016/S0308-8146(99)00089-8
  21. Lee, H. O., Lee, J. W., Lee, S. K., Do, J. H. and Sung, H. S. : Antioxidant effect of Korean red ginseng extract on aqueous linoleic acid and LDL. Agric. Chem. Biotechnol. 40, 283-288 (1997)
  22. Yang, S. J., Woo, K. S., Yoo, J. S., Kang, T. S., Noh, Y. H., Lee, J. S. and Jeong, H. S. : Change of Korean ginseng components with high temperature and pressure treatment. Korean J. Food Sci. Technol. 38, 521-525 (2006)
  23. Kang, K. S., Kim, H. Y., Yamabe, N. and Yokozawa, T. : Stereospecificity in hydroxyl radical scavenging activities of four ginsenosides produced by heat processing. Bioorg. Med. Chem. Lett. 16, 5028-5031 (2006) https://doi.org/10.1016/j.bmcl.2006.07.071
  24. Kang, K. S., Yamabe, N., Kim, H. Y., Okamoto, T., Sei, Y. and Yokozwa, T. : Increase in the free radical scavenging activities of American ginseng by heat processing and its safety evaluation. J. Ethnopharmacol. 113, 225-232 (2007) https://doi.org/10.1016/j.jep.2007.05.027

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