한국자원식물학회지 (Korean Journal of Plant Resources)

  • 제20권1호
  • /
  • Pages.73-78
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  • 2007
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  • 1226-3591(pISSN)
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  • 2287-8203(eISSN)
한국자원식물학회 (The Plant Resources Society of Korea)
권호 표지

장미화분 임성 확인을 위한 효율적인 염색법

Effective Identification of Rose Pollen Fertility using Staining Methods

  • 김기준 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 김희라 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 이자현 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 기광연 (전남농업기술원) ;
  • 이정현 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 한태호 (전남대학교 농업생명과학대학 식물생명공학부) ;
  • 최정근 (전남대학교 농업생명과학대학 식물생명공학부)
  • Kim, Gi-Jun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Kim, Hee-Ra (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Lee, Ja-Hyun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Gi, Gwang-Yeon (Department of Horticulture, Jeonnam Agricultural Research and Extention Services) ;
  • Lee, Jeong-Hyun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Han, Tae-Ho (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University) ;
  • Choi, Jeong-Keun (Division of Plant Biotechnology, College of Agriculture and Life Science, Chonnam National University)
  • 발행 : 2007.02.28
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초록

교배효율의 증대를 위해서는 정상화분의 확보가 선행되어야하며 화분의 활성을 파악할 필요가 있다. 본 연구에서는 화분의 활성을 확인하기 위해 여러 가지 염색액을 사용하여 광학현미경하에서 관찰하였다. Aceto-carmine과 Alexander's 염색액을 사용한 실험에서 비슷한 정상화분율을 얻을 수 있었고 FCR염색으로 관찰한 결과 살아있는 화분만 정확히 선별해 주기에 가장 낮은 정상화분율을 얻을 수 있었다. TB는 화분의 상태에 따라 달랐으나 Aceto-carmine과 Alexander's 염색액을 사용한 실험에서의 결과와 비슷한 양상을 보였다. 화분 관찰의 경험이 별로 없는 실험자도 쉽게 접근할 수 있는 염색액은 Alexander's 이었다. 정확한 화분의 활성을 조사하는 데에는 FCR이 필수이다. 형광현미경 장치 없이 정상과 비정상화분의 구별이 용이한 Alexander's 염색액으로 관찰한 자료를 바탕으로 살아있는 정상화분율의 예견이 가능하나 정확한 임성을 확인하려면 추가로 FCR test를 해야 한다.

Efficient pollination needs abundant fertile pollen in rose breeding. This study was performed to find out efficient staining methods for the detection of fertile pollen. Aceto-carmine and Alexander's stain gave similar results in terms of percentage of normal pollen. Fluorochromatic reaction (FCR) showed the lowest normal pollen percentage because FCR stained only fertile pollen while others stained cytoplasm. Toluidine blue O (TB) showed similar percentage of normal pollen to Aceto-carmine and Alexander's, but could not clearly distinguish the clustered abnormal pollens. Alexander's stain was easy and simple, but difficult to distinguish fertile and infertile pollen. FCR showed only fertile pollen. Alexander's stain showed approximate fertility and FCR showed exact pollen fertility.

키워드

참고문헌

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