Effect of Ozonated Water and Chlorine Water Wash on the Quality and Microbial De-contamination of Fresh-cut Carrot Shreds

오존수 및 염소수 세척이 신선편이 당근의 품질 및 미생물억제에 미치는 영향

  • Kim, Ji-Gang (Postharvest Technology Division National Hortcultural Research Institute) ;
  • Luo, Yaguang (Produce Quality and Safety Laboratory, United States Department of Agriculture) ;
  • Lim, Chai-Il (Postharvest Technology Division National Hortcultural Research Institute)
  • 김지강 (농촌진흥청 원예연구소 저장이용과) ;
  • ;
  • 임채일 (농촌진흥청 원예연구소 저장이용과)
  • Published : 2007.02.28

Abstract

Little information exists on how wash operations affect water quality, or the efficacy of sanitizers on vegetable quality and microbial reduction in fresh-cut carrot shreds. This study evaluated the efficacy of chlorine and ozone in reducing microbial loads and maintaining vegetable quality of carrot shreds. Fresh-cut carrot shreds were teated with various chlorine and ozone concentrations for differing times. The samples were then centrifuged to remove excess water, packaged in film, and stored at $5^{\circ}C$. The result indicated that varying the ozonated water wash time affected microbial growth the development of unpleasant odors, color, and the overall quality of carrot shreds. Ozonated water washing for 20 min maintained vegetable quality by inhibiting unpleasant odors, the development of whiteness, and by reducing microbial populations. A single chlorine water wash was effective and resulted in better vegetable quality when compared with two washes. Samples washed for 20 min in ozonated water, however, had better vegetable quality and smaller microbial counts compared to samples washed once in chlorine water A 20 min ozonated water wash is an attractive method for the maintenance of vegetable quality and shelf-life in fresh-cut carrot shreds.

신선편이 당근채 세척시 오존수 사용에 의한 품질 및 안전성 향상 효과를 구명하기 위해 연구를 수행하였다. 세척 당근을 절단한 뒤 100ppm의 염소수에 1, 2회 세척 및 오존발생장치에서 공급되는 초기 오존수 농도 2ppm에서 1,5, 20분 세척한 뒤 $O_{2}$ 투과율 $29.2pmol{\cdot}s^{-1}{\cdot}m^{-2}{\cdot}Pa^{-1}$ 필름으로 포장하여 $5^{\circ}C$에서 3주간 저장되었다. 당근채의 오존수 세척처리는 저장 중 포장내부의 기체 조성, 당근채의 품질 및 미생물 수에 영향을 미쳤다. 오존수 20분 처리는 당근채 저장 중 $CO_{2}$ 농도가 낮고, 경도가 높았으며, 백화지수(WI), 이취발생 및 미생물 수가 적어 가장 높은 관능점수를 나타내며 저장 3주까지 상품성을 유지하였다. 그러나 염소수 1회 세척은 비록 미생물 수는 오존수 20분 처리와 함께 낮게 나타났으나 품질에 있어서 상대적으로 WI와 이취발생이 높아 오존수 20분 보다 품질변화가 심하였다. 따라서 오존수 20분 처리는 신선편이 당근채의 염소 세척을 대체할 수 있는 방법으로 판단되었다.

Keywords

References

  1. Jacxsens, L., Devlieghere, F., Ragaert, P., Vanneste, E. and Debevere, J. (2003) Relation between microbiological quality, metabolite production and sensory quality of equilibrium modified atmosphere packaged fresh-cut produce. Int. J. Food Microbiology, 83, 263-280 https://doi.org/10.1016/S0168-1605(02)00376-8
  2. Gonzalez R.J., Luo, Y., Ruiz-Cruz, S.and McEvoy, J.L. (2004) Efficacy of sanitizers to inactivate Escherichia coli O157:H7 on fresh-cut carrot shreds under simulated process water conditions. J.Food Protect, 67, 2375-2380 https://doi.org/10.4315/0362-028X-67.11.2375
  3. Khadre, M.A., Yousef, A.E. and Kim, J.G. (2001) Microbiological aspects of ozone applications in food: a review. J. Food Sci. 66, 1242-1252 https://doi.org/10.1111/j.1365-2621.2001.tb15196.x
  4. Xu, L.(1999) Use of ozone to improve the safety of fresh fruits and vegetables. Food Technol., 53, 58-62
  5. Klaiber, R.G., Baur, S., Wolf, G., Hammes, W.P. and Carle, R. (2005) Quality of minimally processed carrots as affected by warm water washing and chlorination. Innovative Food Science and Emerging Technologies, 6, 351-362 https://doi.org/10.1016/j.ifset.2005.03.002
  6. Kim, J.G. (2004) Improvement of washing method of fresh-cut carrot. Postharvest Technology Division, National Horticultural Research Institute Research Report
  7. HACH (2002) Chemical oxygen demand, Method 8000. In: Water analysis handbook. HACH Chemical Company.Loveland, CO, U.S.A. p.1-8
  8. Bolin, H., and Huxsoll C. (1991) Control of minimally processed carrot (Docc ddot) surface discoloration caused by abrasion peeling. J. Food Sci., 56, 416-418 https://doi.org/10.1111/j.1365-2621.1991.tb05293.x
  9. Wang, H., Feng, H. and Luo, Y. (2004) Microbial reduction and storage quality of fresh-cut cilantro washed with acidic electrolyzed water and aqueous ozone. Food Research International, 37, 949-956 https://doi.org/10.1016/j.foodres.2004.06.004
  10. Lopez-Galvez, G., Peiser, G., Nie, X. and Cantwell, M. (1997) Quality changes in packaged salad products during storage. Zeithschrift Lebensmittel-Untersuchung Forschung. 205, 64-72 https://doi.org/10.1007/s002170050125
  11. Meilgaard, M., Civille, G.V. and Carr, B.T. (1991) Sensory evaluation techniques. 2nd ed. CRC Press Inc., Boca Raton, Fla. U.S.A. p.211-217
  12. Bijan, L. and Mohseni, M. (2005) Integrated ozone and biotreatment of pulp mill effluent and changes in biodegradability and molecular weight distribution of organic compounds.Water Research, 39, 3763-3772 https://doi.org/10.1016/j.watres.2005.07.018
  13. Kim, J.G. and Yousef, A.E. (2000) Inactivation Kinetics of foodborne spoilage and pathogenic bacteria by ozone. J. Food Sci., 65, 521-528 https://doi.org/10.1111/j.1365-2621.2000.tb16040.x
  14. Kim, J.G., Luo, Y., Saftner, R.A. and Gross, K.C. (2005) Delayed modified atmosphere packaging of fresh-cut Romaine lettuce: Effects on quality maintenance and shelf-life. J. Amer. Soc. Hort. Sci., 130, 116-120
  15. Ballantyne A., Stark R. and Selman J.D. (1988) Modified atmosphere packaging of shredded lettuce. J. Food Sci. Technol., 23, 267-274
  16. Hong, J.H., Douglas, J.M. Mary, J.C. and Gross. K.C. (2000) Tomato cultivation systems affect subsequent quality of fresh-cut fruit slices. J. Amer. Soc. Hort. Sci., 125, 729-735
  17. Jiang, Y., Shina, T., Nakamura, N. and Nakahara, A. (2001) Electrical conductivity evaluation of postharvest strawberry damage. J. Food Sci., 66, 1392-1395 https://doi.org/10.1111/j.1365-2621.2001.tb15220.x
  18. Kim, J.G., Choi, S.T. and Lim.C.I. (2005) Effect of delayed modified atmosphere packaging on quality of fresh-cut iceberg lettuce. Korean J. Hort. Sci. Technol., 23, 140-145
  19. Kim, J.G., Luo, Y., Yang, T., Saftner, R.A. and Gross, K.C. (2005) Effect of initial oxygen concentration and film oxygen transmission rate on the quality of fresh-cut romaine lettuce. J. Sci. Food and Agri., 85, 1622-1630 https://doi.org/10.1002/jsfa.2158
  20. Luo, Y., McEvoy, J.L., Wachtel, M.R., Kim, J.G. and Huang, Y. (2004) Package atmosphere affects postharvest biology and quality of fresh-cut cilantro leaves. HortScience, 39, 567-570
  21. Li, P. and Barth. M.M. (1998) Impact of edible coatings on nutritional and physiological changes in lightly processed carrots. Postharvest Biol. Technol., 14, 51-60 https://doi.org/10.1016/S0925-5214(98)00020-9
  22. Cameron, A.C., Talasila, P.C. and Joles, D.W. (1995) Predicting film permeability needs fa modified atmosphere packaging of lightly processed fruits and vegetables. HortScience, 30, 25-34
  23. Smyth, A.B., Song, J. and Cameron, A.C. (1998) Modified atmosphere packaged cut iceberg lettuce: Effect of temperature and $O_2$ partial pressure on respiration and quality. J. Agric. Food Chem., 46, 4556-4562 https://doi.org/10.1021/jf980208s