Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Evaluation of Protective Effects of Houttuynia cordata on H2O2-Induced Oxidative DNA Damage Using an Alkaline Comet Assay in Human HepG2 Cells

  • Hah, Dae-Sik (Gyengnam Livestock Promotion Institute Middle-branch) ;
  • Kim, Chung-Hui (Department of Animal Science and Biotechnology, Jinju National University) ;
  • Ryu, Jae-Doo (National Veterinary Research and Quarantine Service Busan Regional Office) ;
  • Kim, Eui-Kyung (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kim, Jong-Shu (Department of Pharmacology & Toxicology, College of Veterinary Medicine, Gyeongsang National University)
  • Published : 2007.03.31
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Abstract

To evaluate the protective effect of Houttuynia cordata on hydrogen peroxide-induced oxidative DNA damage in HepG2 cell line, we used an alkaline single-cell gel electrophoresis (SCGE; comet assay). The DNA damage was analyzed by tail moment (TM) and tail length (TL), which used markers of DNA strand breaks in SCGE. The $100{\mu}g/ml$ of methanolic extract of Houttuynia cordata root showed significant protective effects (p < 0.01) against hydrogen peroxide-induced DNA damage in HepG2 cells and increased cell viability against hydrogen peroxide. The results of this study indicate that Houttuynia cordata root methanol extract acts as a potential antioxidant, and exhibits potential anticancer properties, which may provide a clue to find applications in new pharmaceuticals for oxidative stability.

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References

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