Change of the Amylase Secretion on the Rat Submandibular Gland in the Restraint Stress Condition

구속스트레스에 의한 백서 악하선의 Amylase 분비 변화

  • Koo, Han-Mi (Dept. of Oral Medicine, College of Dentistry, Kyung Hee University) ;
  • Au, Q-Schick (Dept. of Oral Medicine, College of Dentistry, Kyung Hee University) ;
  • Chun, Yang-Hyun (Dept. of Oral Medicine, College of Dentistry, Kyung Hee University) ;
  • Hong, Jung-Pyo (Dept. of Oral Medicine, College of Dentistry, Kyung Hee University)
  • 구한미 (경희대학교 치과대학 구강내과학교실) ;
  • 어규식 (경희대학교 치과대학 구강내과학교실) ;
  • 전양현 (경희대학교 치과대학 구강내과학교실) ;
  • 홍정표 (경희대학교 치과대학 구강내과학교실)
  • Published : 2007.03.30

Abstract

In currently, stress diseases are increased that present several sign and symptoms. Under stress condition, there are dry mouth, burning mouth syndrome, oral mucosa diseases and halitosis more frequently. Changing of salivary proportion is checked in almost patients with changing of function and structure in salivary gland. This study purpose are what effect stress does on salivary gland, and a-amylase on salivary gland. This study was resulted that 1. Under restraint stress, acinar cells are vacuolization and changing of intercellular spaces are separated, and peripheral tissues of duct are changed 2. Acinar cells were shrunk after 3 hours under restraint stress, intercellular space was separated after 6hours, peripheral tissues of duct started to change after 72 hours, and acinar cells and peripheral tissues of duct were all severely changed after 168hours. 3. In immunohistochemical study, amylase reaction was showed partially and irregularly after 3 hours, was getting little milder after 6 hours. And amylase reaction was gradually increased from the time of 12 hours after experiment up to the time of 48 hours after experiment. But after 168 hours, amylase appearance was diminished. According this result, emotional stress can change of salivary gland structure, and amylase secretion, the important digestive enzyme from salivary gland is changed and it is supposed to make digestive disorder and to make halitosis efficiency. So, we need to study about secretion of amylase.

현대인들에게는 다양한 증상과 징후를 나타내는 스트레스성 질환이 증가되고 있는 추세에 있다. 이러한 스트레스 시에 구강 내에는 구강건조이나 구강작열감증후군, 구강점막질환, 구취 등이 호발 된다는 것은 이미 널리 알려진 사실이다. 이때 대부분의 환자에서 공통적으로 나타나는 현상은 타액선의 기능적 및 구조적 변화에 따른 타액성분의 변화이다. 이에 저자는 스트레스가 구강 내 타액선에 어떠한 영향을 미치며, 타액선으로부터 분비되는 ${\alpha}$-amylase에 어떠한 영향을 미치는지를 알아보고자 본 실험을 시행한 결과 다음과 같은 유의성 있는 결과를 얻었다. 1. 구속 스트레스에 의하여 타액선 조직은 선포세포가 공포화되며 변성되었고, 선포간극이 이개되었으며, 도관주위조직도 변성되었다. 2. 구속 3시간 후에는 선포세포가 위축되기 시작하였고, 구속 6시간 후에는 선포 간 이개현상이 관찰되었으며, 구속 72시간 후에는 도관 주위 조직도 변성되기 시작하였고, 점차 심화되어 구속 168시간 후에는 선포와 도관 주위조직이 모두 심하게 변성되었다. 3. 면역조직화학적 검사에서 구속 3시간 후에는 amylase의 발현이 부분적으로 불규칙하게 관찰되었으며, 구속 6시간 후에는 다소 감소되었고, 구속 12시간 후에는 amylase가 부분적으로 응집되었으며, 점차 감소되고 응집되어, 구속 48시간 후에는 전 타액선에 amylase의 응집현상이 심화되었고, 구속 168시간 후에는 amylase의 발현이 매우 감소되었다. 이상의 결과를 바탕으로 정서적인 스트레스를 받으면 타액선이 변성될 수 있으며, 타액선으로부터 분비되는 중요한 소화 효소인 amylase가 변화되어 소화 장애를 일으키거나 구강건강과 관계없이 구취발생에도 영향을 미칠 수 있다고 예측할 수 있어, 향후 amylase의 분비변화에 따른 지속적인 연구가 필요할 것으로 생각된다.

Keywords

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