Journal of Marine Bioscience and Biotechnology (한국해양바이오학회지)

The Korean Society for Marine Biotechnology (한국해양바이오학회)
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Isolation and characterization of the outer membrane vesicle (OMV) protein from Vibrio anguillarum O1

Vibrio anguillarum O1이 생산하는 Outer Membrane Vesicle (OMV)의 분리 및 OMV 내의 단백질 특성

  • Hong, Gyeong-Eun (Department of Biotechnology, Pukyong National University) ;
  • Kim, Dong-Gyun (Department of Biotechnology, Pukyong National University) ;
  • Min, Mun-Kyeong (Department of Biotechnology, Pukyong National University) ;
  • Kong, In-Soo (Department of Biotechnology, Pukyong National University)
  • 홍경은 (부경대학교 수산과학대학 생물공학과) ;
  • 김동균 (부경대학교 수산과학대학 생물공학과) ;
  • 민문경 (부경대학교 수산과학대학 생물공학과) ;
  • 공인수 (부경대학교 수산과학대학 생물공학과)
  • Published : 2007.06.30
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Abstract

Vibrio anguillarum is a gram-negative bacterium that causes vibriosis in approximately 80 different fish species. V. anguillarum produces several exotoxins are correlated with the pathogenesis of vibriosis. This study is focused on the composition of the outer membrane vesicle. Most of gram-negative bacteria produce outer membrane vesicle (OMV) during cell growth. OMV was formed from the outer membrane surface of cell and than released to extracellular environment. OMV consists of outer membrane lipids, outer membrane protein (OMP), LPS, and soluble periplasmic components. Also, they contain toxins, adhesions, and immunomodulatory. Many gram-negative bacteria were studied out forming OMV. In Vibrio sp., formation of OMV by electron microscopy has been reported from V. cholerae and V. parahaemolyticus. In present study, we isolated OMV from V. anguillarum and OMV protein was separated by SDS-PAGE. Magor band was sliced and analyzed by MALDI-TOF. The major protein band of 38kDa was identified as OmpU by MALDI-TOF MS analysis.

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