Journal of Animal Science and Technology

Korean Society of Animal Sciences and Technology (한국축산학회)
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Analysis of SNPs in Bovine CSRP3, APOBEC2 and Caveolin Gene Family

소의 CSRP3, APOBEC2, Caveolin 유전자들의 단일염기다형 분석

  • Bhuiyan, M.S.A. (Division of Animal Science & Resources , Research Center for Transgenic Cloned Pigs, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Yu, S.L. (Division of Animal Science & Resources , Research Center for Transgenic Cloned Pigs, College of Agriculture and Life Sciences, Chungnam National University) ;
  • Kim, K.S. (Department of Animal Science, Chungbuk National University) ;
  • Yoon, D. (Division of Animal Genomics and Bioinformations, National Institute of Animal Science) ;
  • Park, E.W. (Division of Animal Genomics and Bioinformations, National Institute of Animal Science) ;
  • Jeon, J.T. (Division of Animal Science and Technologies, Gyeongsang National University) ;
  • Lee, J.H. (Division of Animal Science & Resources , Research Center for Transgenic Cloned Pigs, College of Agriculture and Life Sciences, Chungnam National University)
  • 삼술부이얀 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 유성란 (충남대학교 농업생명과학대학 동물자원과학부) ;
  • 김관석 (충북대학교 농업생명환경대학 축산학과) ;
  • 윤두학 (축산과학원 동물유전체과) ;
  • 박응우 (축산과학원 동물유전체과) ;
  • 전진태 (경상대학교 대학원 응용생명과학부) ;
  • 이준헌 (충남대학교 농업생명과학대학 동물자원과학부)
  • Published : 2007.12.31
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Abstract

The cysteine and glycine rich protein 3 (CSRP3), apolipoprotein B mRNA editing enzyme catalytic polypeptide‐like 2(APOBEC2) and caveolin (CAV) gene family(CAV1, CAV2, CAV3) have been reported to play important roles for carcass and meat quality traits in pig, mouse, human and cattle. As an initial step, we investigated SNPs in these 5 genes among eight different cattle breeds. Eighteen primer pairs were designed from bovine sequence data of NCBI database to amplify the partial gene fragments. Sequencing results revealed 9 SNPs in the coding regions of three caveolin genes, 1 SNP in CSRP3 and 3 SNPs in APOBEC2 gene. All the identified SNPs were confirmed by PCR-RFLP. Also, 9 more intronic SNPs were detected in these genes. However, all identified mutations in the coding region do not change amino acid sequence. Allelic distributions were significantly different for 5 SNPs in CAV2, CAV3, CSRP3 and APOBEC2 genes among the eight different breeds. These results gave some clues about the polymorphisms of these genes among the cattle breeds and will be useful for further searches for identifying association between these SNPs and meat quality traits in cattle.

CSRP3, APOBEC2, CAV1, CAV2 및 CAV3 유전자들은 포유동물에서 도체와 육질 형질에 중요한 역할을 하는 것으로 보고되고 있다. 따라서, 이 유전자들의 단일염기다형(Single nucleotide poly- morphism; SNP)을 8개의 다른 소의 품종에서 확인한 결과 coding region에서 caveolin family 유전자에서 9개의 SNP, CSRP3유전자에서 1개의 SNP 및 APOBEC2 유전자에서 3개의 SNP가 존재함을 확인하였다. 이 coding region의 SNP들은 PCR-RFLP 방법에 의해 재확인하였으며 이들 유전자의 intronic region에서도 9개의 SNP가 존재함을 확인할 수 있었다. 8개의 다른 품종 소에 각 유전자들의 SNP들을 이용하여 유전자 빈도를 확인한 결과 CAV2, CAV3, CSRP3 및 APOBEC2 유전자의 SNP 중에서 5개가 품종간에서 유의적으로 차이가 있음을 확인할 수 있었다. 이 SNP들은 차후 검증작업을 통하여 육질관련 형질 마커로 이용될 수 있을 것으로 사료된다.

Keywords

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