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Analysis of Single Nucleotide Polymorphisms of Leptin Gene in Hanwoo(Korean Cattle)

한우 Leptin 유전자의 단일 염기 다형성 분석

  • Lee, J.-Min (Department of Animal Biotechnology, Konkuk University) ;
  • Song, G.C. (Department of Animal Biotechnology, Konkuk University) ;
  • Lee, J.Y. (National Genome Research Institute, Korea National Institute of Health) ;
  • Kim, Young-Bong (Department of Animal Biotechnology, Konkuk University)
  • 이정민 (건국대학교 동물생명공학과) ;
  • 송기철 (건국대학교 동물생명공학과) ;
  • 이종영 (국립보건연구원 유전체센터) ;
  • 김영봉 (건국대학교 동물생명공학과)
  • Published : 2007.06.30

Abstract

Leptin, the product of the obese(ob) gene, is an adipocyte-derived hormone for the regulation of whole- body energy storage and energy usage. It has been reported that the homozygous mutations in the gene for leptin(LEP) induce obesity and reduce energy expenditure. In cattle, LEP has significant roles directly or indirectly related with phenotypes such as body weight and fat deposits, therefore SNPs of LEP have been considered important genetic marker to estimate carcass fat content in cattle. In this study, SNPs were screened in LEP(2,222 bp) between intron 1 to 3'-UTR from 24 independent Hanwoo(Korean cattle) by PCR and DNA sequencing. Total 25 SNPs were found and two nonsynonymous SNPs including T1163A(V19E) and G3256A(G132D) were newly detected only from Hanwoo. Among 20 SNPs previously reported in cattle, 16 SNPs were found in Hanwoo; however, the frequencies of some SNPs were significantly different between Hanwoo and western cattle breeds. The other 4 SNPs were not detected from Hanwoo. These Hanwoo specific SNP patterns in LEP will be used in development of molecular marker and application to genetic improvement of Hanwoo.

본 연구는 한우 24두의 혈액으로부터 geno- mic DNA를 추출하여 leptin 유전자의 intron 1부터 3’-UTR 부분까지의 염기 서열을 결정하여 총 25개의 SNPs을 발굴하였다. 발굴된 SNPs 중 16개는 기존의 서양 품종에서도 발견되는 것으로 그 빈도는 대부분의 경우 유사하였으나, 서로 유의성이 있도록 상이한 경우도 있었다. Intron 1의 T1064G, exon 2에서 발견된 nonsynonymous SNP(T1163A, Val to Glu)과 exon 3의 C3011G 및 G3256A(Gly to Asp) SNPs은 기존에 보고되지 않은 한우에서 새롭게 발견된 것으로 종 간의 차이를 나타내는 것으로 사료된다. 이러한 한우의 SNPs 정보는 한우의 유전형을 결정하고, 도체 및 육질 형질 등 중요 경제 형질과의 연관 분석을 통하여 중요한 유전자 표지 확보 및 한우 판별 등의 응용에 이용될 수 있을 것으로 사료된다.

Keywords

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