Journal of the Korean Society of Food Science and Nutrition (한국식품영양과학회지)

The Korean Society of Food Science and Nutrition (한국식품영양과학회)
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The Effects of Plebeiae Herba (Salvia plebeia R. Br.) on the Anticancer (in vitro) and Activation of Immune Cells

여지초의 in vitro항암효과 및 면역세포 활성화에 미치는 영향

  • Bae, Man-Jong (Dept. of Herbal Foodceutical Science, Daegu Haany University) ;
  • Ye, Eung-Ju (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technolopark) ;
  • Kim, Soo-Jung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technolopark) ;
  • Kim, Jae-Myoung (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technolopark) ;
  • Yee, Sung-Tae (Dept. of Biology, Sunchon National University) ;
  • Park, Eun-Mi (Efficacy and Safety Research Center for Traditional Oriental Medicine, Daegu Hanny University, Kyongbuk Technolopark)
  • 배만종 (대구한의대학교 한방식품약리학과) ;
  • 예은주 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김수정 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 김재명 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원) ;
  • 이성태 (순천대학교 생물학과) ;
  • 박은미 ((재)경북테크노파크 대구한의대학교 한방생명자원특화센터 효능검증원)
  • Published : 2007.04.30
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Abstract

This study was designed to investigate the effect of Plebeiae Herba (Salvia plebeia R. Br.) on the proliferation of AGS cell lines and the activation of splenocytes. In an anti-cancer test using AGS cells, water and ethanol extracts of Plebeiae Herba inhibited the growth of AGS cell lines and morphological changes were also observed in a dose-dependent manner. Water extract of Plebeiae Herba showed growth-inhibitory effect of 43.3% at $1,000{\mu}g/mL$ and 69.7% at $3,000{\mu}g/mL$. Ethanol extracts of Plebeiae Herba showed growth-inhibitory effect of approximately 37.3% for $1,000{\mu}g/mL$ and 75.8% for $3,000{\mu}g/mL$. The Plebeiae Herba induced the proliferation of spleen cells and increased interleukin (IL)-2, interleukin (IL)-6 and tumor necrosis factor $(TNF)-{\alpha}$. In conclusion, these results suggest that the Plebeiae Herba seems to have antiproliferationg effect against the AGS cell and acts as a potent immunomodulator.

본 연구에서는 여지초의 cell line상에서의 항암효과(in vitro) 및 면역세포 활성화에 미치는 영향을 검토하여 다음과 같은 결과를 얻었다. 인체유래 위암세포주인 AGS의 형태변화는 여지초 물과 60% 에탄을 추출물 처리 시 농도에 비례하여 세포의 밀도가 감소하였고 세포의 원형화나 손실이 증가하였다. 암세포 주의 증식에 미치는 영향은 AGS의 경우 농도에 비례하여 암세포 증식 억제율이 나타났으며, 물 추출물 $1,000{\mu}g/mL$에서 43.3%, $3,000{\mu}g/mL$에서 69.7%, 60% 에탄을 추출물 $1,000{\mu}g/mL$에서 37.3%, $3,000{\mu}g/mL$에서 75.8%의 억제율을 나타내었다. 여지초 추출물이 비장세포 증식에 미치는 영향은 여지초 물 추출물은 $30{\mu}g/mL$, 60% 에탄을 추출물은 $10{\mu}g/mL$에서 각각 25.0%와 43.2%의 증식반응 증가가 나타났으며, $300{\mu}g/mL$에서는 비장세포의 증식이 무처리 대조군에 비해 낮은 경향을 나타내었다. 여지초 추출물이 비장세포의 사이토카인 생산에 미치는 영향은 IL-2의 경우 여지초 물 추출물 $1{\mu}g/mL$처리군에서 $20.75{\pm}3.54pg/mL$, 60% 에탄을 추출물은 $100{\mu}g/mL$ 처리군에서 $20.75{\pm}7.07pg/mL$로서 $15.75{\pm}7.07 pg/mL$인 무처리 대조군에 비해 모두 31.7%의 증가를 나타내었다 IL-6 분비량 또한 물 추출물 $1{\mu}g/mL$에서 $31.75{\pm}15.91 pg/mL$, 60% 에탄을 추출물 $10{\mu}g/mL$에서 $14.25{\pm}1.77pg/mL$로서 무처리 대조군에 비해 유의한 증가를 보였다. 그리고 물과 60% 에탄을 추출물 모두 $10{\mu}g/mL$에서 무처리 대조군에 비해 각각 69.6%, 25.3%의 $TNF-{\alpha}$ 생산 증가를 유도하였다.

Keywords

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