Optimization of Lipase Pretreatment Prior to Lipase Immobilization to Prevent Loss of Activity

  • Lee, Dong-Hwan (Department of Chemical and Biological Engineering, Korea University) ;
  • Kim, Jung-Mo (Department of Chemical and Biological Engineering, Korea University) ;
  • Shin, Hyun-Yong (Department of Chemical and Biological Engineering, Korea University) ;
  • Kim, Seung-Woo (Department of Chemical and Biological Engineering, Korea University)
  • Published : 2007.04.30

Abstract

In our previous work, a method of pretreating lipase was developed to prevent loss of its activity during covalent immobilization. In this study, Rhizopus oryzae lipase was pretreated before immobilization and then immobilized on a silica gel surface. The effects of the various materials and conditions used in the pretreatment stage on the activity of immobilized lipase were investigated. Immobilized lipase pretreated with 0.1% of soybean oil had better activity than those pretreated with other materials. The optimal temperature, agitation speed, and pretreating time for lipase pretreatment were determined to be $40^{\circ}C$, 200rpm, and 45min, respectively. The activity of immobilized soybean oil pretreated lipase was 630U/g matrix, which is 20 times higher than that of immobilized non-pretreated lipase. In addition, immobilized lipase activity was maintained at levels exceeding 90% of its original activity after 10 reuses.

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