Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Activation and In Vitro Development of Porcine Oocytes Treated with Ethanol, $Ca^{2+}-Ionophore$ and Strontium

Ethanol, $Ca^{2+}-Ionophore$ 및 Strontium이 돼지 난자의 활성화와 체외 발달에 미치는 영향

  • Ahn, H.J. (Department of Animal Science, Insti. of Ag. Sci and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Lee, J.W. (Department of Animal Science, Insti. of Ag. Sci and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Kang, M.J. (Department of Animal Science, Insti. of Ag. Sci and Tech., College of Agriculture & Life Science, Chonnam National University) ;
  • Moon, S.J. (Department of Animal Science, Insti. of Ag. Sci and Tech., College of Agriculture & Life Science, Chonnam National University)
  • 안현정 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소) ;
  • 이지웅 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소) ;
  • 강만종 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소) ;
  • 문승주 (전남대학교 농업생명과학대학 동물자원학부, 농업과학기술연구소)
  • Published : 2007.03.31
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Abstract

The objective of this study was to examine the optimal concentration and the exposure time of ethanol, Ca-ionophore, and strontium to achieve massive recipient oocytes in porcine. The cleavage (51.4% vs. $21.3{\sim}44.3%$) and embryo development rates (45% vs. $13.3{\sim}29.9%$) were significantly higher (p.0.05) in oocytes treated with 10% ethanol for 10 min than other treatments. The oocytes treated with 25mM Ca-ionophore for a minimum of 2min and 20mM strontium for a minimum of 6h showed significantly higher cleavage and embryo development rates than those of other treatments (P<0.05). Cleavage rate with duplicated ethanol treatment was significantly lower than those with ethanol alone (P<0.05). The cleavage rate and embryo development rates were significantly lower in duplicated strontium treatment than those in both alone and combination (P<0.05). But the cleavage and embryo development rates in treatment with Ca-ionophore were significantly higher in combined treatment (Ca-ionophore and cycloheximide) than those in single or duplicated treatment (P<0.05). These results might induce establishment of the optimal concentration and the exposure time on activation media to build up activation condition of porcine oocytes.

본 연구는 효율적인 돼지 난자의 활성화를 통한 수핵란의 대량 확보를 위하여 ethanol, Ca-ionophore 및 strontium의 최적 농도 및 노출 시간을 규명하기 위하여 실시되었다. Ethanol은 10%에서 10분간 노출시켰을 때 난할율과 배발달 성적이 각각 51.4%와 45%로 다른 처리구에 비하여 유의적으로 높았으며 (P<0.05), Ca-ionophore는 $25{\mu}M$에서 2분간 노출시켰을 때 난할율과 배발달 성적이 유의적으로 높았다. 또한 strontium은 20mM에서 6시간 노출시켰을 때 난할율과 배발달 성적 모두 유의적으로 높았다(P<0.05). Strontium중복 처리는 단독 및 병용처리 할 때보다 난할율과 배발달율 모두 유의 적으로 낮았다(P<0.05). 이러한 결과는 돼지 난자의 활성화 조건을 확립하기 위한 활성화제의 최적 농도와 노출 시간 확립에 기여할 수 있으리라 생각된다.

Keywords

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