Study on Effectiveness of Korean Medicine Therapy and the Relation between Effectiveness of that and Single Nucleotide Gene Polymorphism in Stroke Patients

중풍의 한방치료 효과와 단일염기유전자다형성과의 연관성 연구

  • Lee, Yoon-Kyoung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dae-gu Haany University) ;
  • Kim, Jae-Su (Kiumi Orietal Medical Clinic) ;
  • Lee, Pyeong-Jae (Department of Natural Medicine Resources, Semyung University)
  • 이윤경 (대구한의대학교 한의과대학 침구학교실) ;
  • 김재수 (키우미한의원) ;
  • 이평재 (세명대학교 한방자연약재과학과)
  • Published : 2007.10.25


This study was designed to investigate effectiveness of korean medicine therapy and the relation between effectiveness of that and single nucleotide gene polymorphism in stroke patients. This study was carried out on 92 stroke patients who were admitted to the department of acupuncture & moxibustion, college of Oriental medicine, Daegu Haany University and 112 healthy Korean. All patients were received Korean medicine therapy including acupuncture and herbal medicine for stroke and assessed by National Institutes of Health Stroke Scale(NIHSS). Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerase chain reaction(PCR). PCR products were visualized by 1.5% agarose gel electrophoresis. Through Pyrosequencing of PCR product, the polymorphism of single nucleotide gene was genotyped automatically. There were significant difference between before and after Korean medicine therapy in NIHSS. Genotypes were AA, AG, GG, but there was no significant difference between control and stroke groups. And there was not any statistical significant allelic frequency difference between control and stroke groups. We concluded that Korean medicine therapy in stroke patient can improve NIHSS, but there is no definite relation between effectiveness of Korean medicine therapy and single nucleotide gene polymorphism in stroke patients. This study need to be confirmed in large patients and further studies about relation with gene polymorphism are required.


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