Study on Effectiveness of Korean Medicine Therapy and the Relation between Effectiveness of that and Single Nucleotide Gene Polymorphism in Stroke Patients

중풍의 한방치료 효과와 단일염기유전자다형성과의 연관성 연구

  • Lee, Yoon-Kyoung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dae-gu Haany University) ;
  • Kim, Jae-Su (Kiumi Orietal Medical Clinic) ;
  • Lee, Pyeong-Jae (Department of Natural Medicine Resources, Semyung University)
  • 이윤경 (대구한의대학교 한의과대학 침구학교실) ;
  • 김재수 (키우미한의원) ;
  • 이평재 (세명대학교 한방자연약재과학과)
  • Published : 2007.10.25

Abstract

This study was designed to investigate effectiveness of korean medicine therapy and the relation between effectiveness of that and single nucleotide gene polymorphism in stroke patients. This study was carried out on 92 stroke patients who were admitted to the department of acupuncture & moxibustion, college of Oriental medicine, Daegu Haany University and 112 healthy Korean. All patients were received Korean medicine therapy including acupuncture and herbal medicine for stroke and assessed by National Institutes of Health Stroke Scale(NIHSS). Blood samples from all subjects were obtained for DNA extraction. The extracted DNA was amplified by polymerase chain reaction(PCR). PCR products were visualized by 1.5% agarose gel electrophoresis. Through Pyrosequencing of PCR product, the polymorphism of single nucleotide gene was genotyped automatically. There were significant difference between before and after Korean medicine therapy in NIHSS. Genotypes were AA, AG, GG, but there was no significant difference between control and stroke groups. And there was not any statistical significant allelic frequency difference between control and stroke groups. We concluded that Korean medicine therapy in stroke patient can improve NIHSS, but there is no definite relation between effectiveness of Korean medicine therapy and single nucleotide gene polymorphism in stroke patients. This study need to be confirmed in large patients and further studies about relation with gene polymorphism are required.

References

  1. 대한신경외과학회. 신경외과학. 서울, 중앙기획사, 263, 2002
  2. 통계청. 2005년 사망원인 통계결과. 2006 Sep. (Available from :URL:http://meta.nso.go.kr/metaSearch/metasearch1.jsp.)
  3. Evans, R.W., Baskin, D.S., Yatsu, F.M. Prognosis os neurological disorders. Oxford, Oxford university press. 73, 2000
  4. 김영석. 임상중풍학. 서울, 서원당, pp 62-64, 303-304, 308, 349-350, 1997
  5. Szolnoki, Z., Somogyvari, F., Szabo, M., Fodor, L. A clustering of unfavorable common genetic mutations in stroke cases. Acta Neurol Scand. 102: 124-128, 2000 https://doi.org/10.1034/j.1600-0404.2000.102002124.x
  6. 서정철, 김종인, 김윤미, 남동현, 백용형, 최봉근, 김연정, 김창주, 정주호, 인창식, 김이화, 한상원, 고형균, 김창환. 한국인의 중풍 환자의 Serotonin Transporter 유전자다형성-환자 대조군 연구 277례. 대한침구학회지 19(1):111-117, 2002
  7. 이진우, 이경진, 노삼웅, 김재종, 배형섭, 홍무창, 신민규, 김영석, 배현수. 한국인의 ACE 유전자 다형성과 뇌혈관 질환과의 관계에 대한 연구. 동의생리병리학회지 16(4):724- 728, 2002
  8. Muir, K.W., Weir, C.J., Murray, G.D., Povey, C., Lees, K.R. Comparison of neurological scales and scoring systems for acute stroke prognosis. Stroke. 27: 1817-1820, 1996 https://doi.org/10.1161/01.STR.27.10.1817
  9. Lesch, K.P., Bengel, D., Heils, A. Association of anxiety-related traits with a polymorphism in the serotonin transporter gene regulatory region. Science. 274: 1527-1530, 1996 https://doi.org/10.1126/science.274.5292.1527
  10. http://www.pyrosequencing.com
  11. Gruber, J.D., Colligan, P.B., Wolford, J.K. Estimation of single nucleotide polymorphism allele frequency in DNA pools by using Pyrosequencing. Hum Genet. 110: 395- 401, 2002 https://doi.org/10.1007/s00439-002-0722-6
  12. Pettersson, M., Bylund, M., Alderborn, A. Molecular haplotype determination using allele-specific PCR and pyrosequencing technology. Genomics. 82: 390-396, 2003 https://doi.org/10.1016/S0888-7543(03)00177-0
  13. Pacey-Miller, T., Henry, R. Single-nucleotide polymorphism detection in plants using a single-stranded pyrosequencing protocol with a universal biotinylated primer. Anal Biochem. 317: 166-170, 2003 https://doi.org/10.1016/S0003-2697(03)00089-7
  14. 이민철, 지제근. 신경병리학. 전남, 전남대학교출판부, pp 191-192, 1999
  15. 전중선, 전세일, 박승현, 백소영, 김동아. 뇌졸중의 최근 역학적 동향. 대한재활의학회지 22: 1159-1165, 1998
  16. Duncan, P.W., Goldstein, L.B., Horner, R.D., Landsman, P.B., Samsa, G.P., Matchar, D.B. Similar motor recovery of upper and lower extremities after stroke. Stroke. 25(6):1181-1188, 1994 https://doi.org/10.1161/01.STR.25.6.1181
  17. Jongbloed, L. Prediction of function after stroke : a critical review. Stroke. 17(4):765-766, 1986 https://doi.org/10.1161/01.STR.17.4.765
  18. 박정미, 박창일, 조경자, 신정순. 뇌졸중의 재활치료에 대한 고찰. 대한재활의학회지 11(2):161-172, 1987
  19. Alexander MP. Stroke rehabilitation outcome. Stroke. 25(1):128-134, 1994 https://doi.org/10.1161/01.STR.25.1.128
  20. Nicolas, V., Victor, O., Marian, R., Rafael, O., Angel, C. ${\alpha}1$-antichymotrypsin gene polymorphism in patients with stroke. Stroke. 31: 2103-2105, 2000 https://doi.org/10.1161/01.STR.31.9.2103
  21. Daisuke, I., Mitsuru, M., Norio, T., Hideki, S., Akira, S., Ikuo, S., Kiyoaki, W., Yasuo, F. Polymorphism in promotor of lipopoly -saccharide receptor CD14 and ischemic cerebrovascular disease. Stroke. 31: 2661-2664, 2000 https://doi.org/10.1161/01.STR.31.11.2661
  22. Bugawan, T.L., Mirel, D.B., Valdes, A.M., Panelo, A., Pozzilli, P., Ealich, Ha. Association and interaction of the IL4R, IL4, and IL13 loci with type 1 diabetes among Filipinos. Am J Hum Genet. 72(6):1505-1514, 2003 https://doi.org/10.1086/375655
  23. Mirel, D.B., Valdes, A.M., Lazzeroni, L.C., Reynolds, R.L., Erlich, H.A., Noble, J.A. Association of IL4R haplotypes with type 1 diabetes. Diabetes. 51(11):3336-3341, 2002 https://doi.org/10.2337/diabetes.51.11.3336
  24. Bidwell, J., Keen, L., Gallagher, G., Kimberly, R., Huizinga, T., McDermott, M.F., Oksenberg, J., McNicholl, J., Pociot, F., Hardt, C., D'Alfonso, S. Cytokine gene polymorphism in human disease: on-line databases. Genes Immun. 1: 3-19, 1999 https://doi.org/10.1038/sj.gene.6363645
  25. Olavesen, M.G., Hampe, J., Mirza, M.M., Saiz, R., Lewis, C.M., Bridger, S., Teare, D., Easton, D.F., Herrmann, T., Scott, G., Hirst, J., Sanderson, J., Hodgson, S.V., Lee, J., macPherson, A. Analysis of single nucleotide polymorphisms in the interleukin-4 receptor gene for association with inflammatory bowel disease. Immunogenetics. 51(1):1-7, 2000 https://doi.org/10.1007/s002510050001
  26. Buchs, N., Silvestri, T., di Giovine, F.S., Chabaud, M., Vannier, E., Duff, G.W., Missec, P. IL-4 VNTR gene polymorphism in chronic polyarthritis. The Rare allele is associated with protection against destruction. Rheumatology(Oxford). 39(10):1126-1131, 2000 https://doi.org/10.1093/rheumatology/39.10.1126
  27. Missec, P., Chomarat, P., Dechanet, J., Moreau, J.F., Roux, J.P., Delmas, P., Banchereau, J. Interleukin 4 inhibits bone resorption through an effect on osteoclasts and proinflammatory cytokines in an ex vivo model of bone resorption in rheumatoid arthritis. Arthritis Rheum. 37: 1715-1722, 1994 https://doi.org/10.1002/art.1780371202
  28. Joosten, L.A., Lubberts, E., Durez, P., Helsen, M.M., Jacobs, M.J., Goldman, M. etal. Role of interleukin-4 and interleukin-10 in murine collagen-induced arthritis. Protective effect of interleukin-4 and interleukin-10 treatment on cartilage destruction. Arthritis Rheum. 40: 249-260, 1997 https://doi.org/10.1002/art.1780400209
  29. Hershey, G.K., Friedrich, M.F., Esswein, L.A., Thomas, M.L., Chatila, T.A. The association of atopy with a gain of function mutation in the alpha subunit of the interleukin-4 receptor. New Engl J Med. 337: 1720-1725, 1997 https://doi.org/10.1056/NEJM199712113372403
  30. 한상원, 임성철, 정태영, 신동훈, 김민정, 서정철. pyrosequencing을 이용한 한국인 중풍 환자의 Interleukin 4 Receptor(IL-4R) 유전자 다형성. 대한침구학회지 21(2):21- 29, 2004