Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
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Hepatoprotective Effect of Catechin Isolated from the Root of Rosa rugosa Thunb

해당화 뿌리에서 분리한 Catechin의 간보호효과

  • Hur, Jong-Moon (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University) ;
  • Kim, In-Ho (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University) ;
  • Park, Jong-Cheol (Department of Oriental Medicine Resources and Research Institute of Korean Oriental Medicine, Sunchon National University)
  • 허종문 (순천대학교 한약자원학과 및 한의약연구소) ;
  • 김인호 (순천대학교 한약자원학과 및 한의약연구소) ;
  • 박종철 (순천대학교 한약자원학과 및 한의약연구소)
  • Published : 2007.02.28
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Abstract

The root of Rosa rugosa has been used in folkloric medicine as a treatment agent for diabetes. In the present study, we investigated whether (+)-catechin isolated from this plant can change the activities of hepatic drug metabolizing enzymes in rats treated with bromobenzene. Pretreatment with (+)-catechin gave no effects on the activities of aminopyrine N-demethylase and aniline hydroxylase, enzymes forming toxic bromobenzene epoxide intermediates and glutathione Stransferase, an enzyme removing toxic epoxides. However, the activity of epoxide hydrolase, an enzyme detoxifying the bromobenzene toxic intermediates was mildly recovered by (+)-catechin treatment.

해당화 뿌리는 우리나라 민간에서 당뇨병 치료제로 사용되는 약용식물이다. Bromobenzene으로 간독성을 유발한 흰쥐에 뿌리에서 분리한 화합물인 (+)-catechin을 경구투여하여 bromobenzene대사계에 미치는 효소활성을 간독성 물질인 bromobenzene 3,4-oxide 생성에 관여하는 효소인 aminopyrine N-demetylase와 aniline hydroxylase와 독성 epoxide 대사중간체를 무독화 시키는 epoxide hydrolase와 glutathione S-transferase에 활성을 관찰하였다. (+)-Catechin의 투여가 aminopyrine N-demetylase, aniline hydroxylase 및 glutathione S-transferase에 활성에는 영향을 주지 못하였으나, epoxide hydrolase는 positive control로 사용한 ascorbic acid에 미치지 못하지만, bromobenzene 처리군 보다 39% 효소활성을 회복 시켰다. 따라서, (+)-catechin은 간독성 물질을 무독화시키는 epoxide hydrolase의 활성을 회복시켜 간보호 활성을 나타냄을 알 수 있었으며, 해당화에서 분리한 사포닌 성분인 rosamultin도 이효소의 활성을 증가시킴으로 인해 보호활성을 나타내는 것으로 보고된바 있다.

Keywords

References

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