Environmental Engineering Research

Korean Society of Environmental Engineers (대한환경공학회)
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EVALUATION OF MICROBIAL RISK IN SOIL AMENDED WITH ORGANIC FERTILIZERS FROM STABILIZED SWINE MANURE WASTE

  • Han, Il (Department of Civil Engineering and Environmental Sciences, Korea Military Academy) ;
  • Lee, Young-Shin (Department of Environmental Engineering, Hanseu University) ;
  • Park, Joon-Hong (Department of Civil and Environmental Engineering, Yonsei University)
  • Published : 2007.09.28
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Abstract

This study evaluated microbial risk that could develop within soil microbial communities after amended with organic fertilizers from stabilized swine manure waste. For this purpose, we assessed the occurrences and competitiveness of antibiotic resistance and pathogenicity in soil microbial communities that were amended with swine manure wastes stabilized by a traditional lagoon fermentation process and an autothermal thermophilic aerobic digestion process, respectively. According to laboratory cultivation detection analysis, soil applications of the stabilized organic fertilizers resulted in increases in absolute abundances of antibiotic resistant bacteria and of two tested pathogenic bacteria indicators. The increase in occurrences might be due to the overall growth of microbial communities by the supplement of nutrients from the fertilizers. Meanwhile, the soil applications were found to reduce competitiveness for various types of antibiotic resistant bacteria in the soil microbial communities, as indicated by the decrease in relative abundances (of total viable heterotrophic bacteria). However, competitiveness of pathogens in response to the fertilization was pathogens-specific, since the relative abundance of Staphylococcus was decreased by the soil applications, while the relative abundance of Salmonella was increased. Further testes revealed that no MAR (multiple antibiotic resistance) occurrence was detected among cultivated pathogen colonies. These findings suggest that microbial risk in the soil amended with the fertilizers may not be critical to public health. However, because of the increased occurrences of antibiotic resistance and pathogenicity resulted from the overall microbial growth by the nutrient supply from the fertilizers, potential microbial risk could not be completely ruled out in the organic-fertilized soil samples.

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References

  1. Walsh, C., Antibiotics Actions, Origins, Resistance, ASM Press, pp. 64-66, 87; 222-226 (2003)
  2. Lewis, R., 'The rise of antibiotic-resistant infections,' FDA Consumer Magazine, USFDA, MD, 29(7), (1995)
  3. Hall, B. G., 'Predicting the evolution of antibiotic resistance genes,' Nature Review Microbiology, 2, 430-435 (2004) https://doi.org/10.1038/nrmicro888
  4. Lipsitch, M., Singer, R. S., and Levin, B. R., 'Antibiotics in agriculture: When is it time to close the barn door?' PNAS, 99(9), 5752-5754 (2002) https://doi.org/10.1073/pnas.092142499
  5. Christian, T., Schneider, R. J., Farver, H. A., Skutlarek, D., Meyer, M. T., and Goldbach, H. E., 'Determination of antibiotic residues in manure, soil and surface waters,' Acta Hydrochem, 31 (1), 36-44 (2003) https://doi.org/10.1002/aheh.200390014
  6. Division for Sustainable Development, 'Changing consumption and product patterns: Organic agriculture - Background Paper 4,' Commission on Sustainable Development 8th Session, Department of Economic and Social Affair, UN, New York (2000)
  7. Chee-Sanford, J. G., Aminov, R. I., Krapac, I. J., Garrigues-Jeanjean, N., and Mackie, R. I., 'Occurrence and diversity of tetracycline resistance genes in lagoon and groundwater underlying two swine production facilities,' Appl. Environ. Microbiol., 67(4), 494-1502 (2001)
  8. Kolpin, D. W., Furlong, E. T., Meyer, M. T., Thurman, E. M., Zaugg, S. D., Barber, L. B., and Buxton, H. T., 'Pharmaceuticals, hormones, and other organic wastewater contaminants in U.S. streams, 1999-2000: a national reconnaissance,' Environ. Sci. Technol., 36, 1202-1211 (2002)
  9. Park, B. G., Park, W. G., Sho, K. H., K won, S. I., Nam, J. J., and Cho, N. S., 'Compost Production,' Compost production and usage for environmentally friendly agricultur, Jeon, S. C.(Eds.), Rural Development Administration, Sewon, pp. 21-53 (2002)
  10. Kim, C. G., Kim, T. Y., and Sea, S. C., Analysis of consumer preferences and purchasing behavior towards environmentally friendly agricultural products, Korea Rural Economic Institute, Seoul, pp. 3-7; 26-70 (2005)
  11. Shim, J. D., Okada, Y., Gejirna, Y., and Lee, C., 'Development of the contaminants removal system in aeration basin on livestock wastewater treatment-The performance of a multi-stage treatment reactor for continuous flow treatment-,' Environ. Eng. Res., 4( 1), 31-40 (1999)
  12. Han, I., Microbial evaluation of antibiotic resistance and pathogenicity in autothermal aerobic digestion - treated manure waste, Master's thesis, Yonsei University, (2005)
  13. Staton, K. L., Alleman, E. A., Pressley, R. L., and Eloff, J., 'The second generation autothermal thermophilic aerobic digestion: conceptual issues and process advancement,' in Proceedings of the 2001 WEF/AWWA/CWEA Joint Residuals and Biosolids Management Conference (2001)
  14. Dahab, M. F., and Surampalli, R. Y., 'Effects of aerobic and anaerobic digestion systems on pathogen and pathogen indicator reduction in municipal sludge,' Water Sci. Technol., 46, 181-187 (2002) https://doi.org/10.2166/wst.2002.0325
  15. Koch, A. L., 'Growth Measurement,' Methods for General and Molecular Bacteriology, Gerhardt, P., Murray, R. G. E., Wood, W. A., and Krieg, N. R. (Eds.), American Society for Microbiology, Washington D.C., pp. 254-257 (1994)
  16. Sambrook, J., and Russel, D. W., Molecular Cloning a Laboratory Manual, 3rd ed, vol. 3, CSHL Press, New York, pp A 1.5; A2.6-2.7 (2000)
  17. Friewer, F. I., and Leifson, E., 'Non-motile flagellated variants of Salmonella thyphimurium' J. Pathol. Bacteriol., 64, 223-224 (1952) https://doi.org/10.1002/path.1700640123
  18. Chapman, G. H., 'The value of concentrated human whole blood and agar cultures for testing the coagulating power of Staphylococci,' J. Bacteriol., 50, 234 (1945)

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